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The Histochemical Journal

, Volume 5, Issue 4, pp 291–301 | Cite as

Histochemical investigations of the accumulation of lipids in heart papillary muscle

  • M. Alavaikko
  • J. Hirvonen
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  • 24 Downloads

Synopsis

The lipid composition and fatty change of the papillary muscle, arterioles and chordae tendineae of the left cardiac ventricle of sixty-two patients who had died of various diseases in hospital were analysed qualitatively by histochemical techniques and thin-layer chromatography.

The histochemical techniques revealed considerable differences in the fatty change of the myocardium, arterioles and chordae tendineae. The myocardial change seems to be due solely to accumulation of triglycerides. The fatty change in the chordae tendineae appears to be the result mainly of accumulation of cholesterol esters and, to some extent, of free fatty acids. The arteriolar fatty change arises mainly from accumulation of cholesterol esters and some triglycerides. The myocardial and arteriolar fatty change is greatest at the tip of the papillary muscle.

The results of thin-layer chromatography showed that the lipid composition of the papillary muscle was qualitatively highly constant. The triglycerides and free fatty acid content varied.

The osmium tetroxide-α-naphthylamine technique, which indicates phospholipids, stained the cardiac and arteriolar muscle fibres uniformly. No focal staining, suggestive of fatty change, was noted.

All three tissue sites studied reacted with Baker's acid Haematein test. Staining was focal and clearly associated with fatty change. The results observed suggested that Baker's acid Haematein test is not specific for phospholipids, but it may stain neutral fats and cholesterol esters whenever large amounts of these are present in a tissue.

Keywords

Triglyceride Free Fatty Acid Osmium Lipid Composition Fatty Acid Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adams, C.W.M. (1967). Atheroma lipids.J. Atheroscler. Res. 7, 117–19.Google Scholar
  2. Adams, C.W.M. (1965).Neurohistochemistry, pp. 6–66. Amsterdam: Elsevier.Google Scholar
  3. Adams, C.W.M. (1967).Vascular Histochemistry. London: Lloyd-Luke.Google Scholar
  4. Adams, C. W. M., Abdulla, Y. H., Bayliss, O. B. &Weller, O. R. (1966). Histochemical detection of triglyceride esters with specific lipases and a calcium-lead sulphide technique.J. Histochem. Cytochem. 14, 385–95.Google Scholar
  5. Alavaikko, M., Hirvonen, J. &Räsänen, O. (1970). Fatty change in papillary heart muscle and in its arterioles. Analysis of a material of 262 autopsies.Acta path. microbiol. scand. Section A.78, 458–72.Google Scholar
  6. Elleder, M. &Lojda, Z. (1970). Studies in lipid histochemistry II. The nature of the material stained with acid haematein test and with OTAN reaction in red blood cells.Histochemie 24, 21–8.Google Scholar
  7. Folch, J., Lees, M. &Stanley, G. H. S. (1957). A simple method for the isolation and purification of total lipides from animal tissue.J. biol. Chem. 226, 497–509.Google Scholar
  8. Ho, K.-J. &Taylor, B. (1968). Comparative studies on tissues cholesterol.Arch. Path. 86, 585–96.Google Scholar
  9. Holczinger, L. &Balint, Z. (1962). Experimental data to the theory of fat staining.Histochemie 2, 389.Google Scholar
  10. Kanwar, C. (1960). On the unspecificity of acid haematein technique for the histochemical detection of phospholipids.Microscope 12, 316–19.Google Scholar
  11. Lindlar, F. &Zahi, I. A. (1966). Lipidchemische Untersuchungen zur Frage der degeneraniven Myokardverfettung.Virchows Arch. path. Anat. 341, 142–7.Google Scholar
  12. Mallory, F. B. (1942).Pathological Technique, p. 118. Phidelphia: Saunders.Google Scholar
  13. Pearse, A. G. F. (1968).Histochemistry. Theoretical and Applied. 3rd Edn. Vol. I. London: J. & A. Churchill.Google Scholar
  14. Schmid, H. H. O. (1968). The Alk-i-enyl ether and alkyl ether lipids of bovine heart muscle.Biochim. biophys. Acta 161, 141–7.Google Scholar
  15. Wheeldon, L. W., Schumert, Z. &Turner, D. A. (1965). Lipid composition of heart muscle homogenates.J. Lipid Res. 6, 481–9.Google Scholar
  16. Zugibe, F. T. (1970).Diagnostic Histochemistry, pp. 55–6. St Louis: Mosby.Google Scholar

Copyright information

© Chapman and Hall Ltd 1973

Authors and Affiliations

  • M. Alavaikko
    • 1
  • J. Hirvonen
    • 1
  1. 1.Department of Pathology and Forensic MedicineUniversity of OuluOuluFinland

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