Synopsis
The distribution and activities of several oxidative enzymes in various regions of the sebaceous glands of the domestic cat have been studied. The results obtained emphasize the outstanding importance of NADP-linked dehydrogenases for lipogenesis during sebum production. In particular, the reactions for glucose-6-phosphate dehydrogenase were very strong. Among the NAD-linked dehydrogenases investigated, lactate dehydrogenase showed strong activity in the peripheral cells of the sebaceous gland. The reactions for cytochrome oxidase and succinate dehydrogenase were weaker.
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References
Ball, E. G. (1966). Regulation of fatty acid synthesis in adipose tissue.Adv. Enzyme Reg. 4, 3–18.
Burstone, M. S. (1960). Histochemical demonstration of cytochrome oxidase with new amine reagents.J. Histochem. Cytochem. 8, 63–70.
Dunn, J. D. &Lin, F. J. (1974). Daily fluctuations in adrenal catecholamine concentration.Experientia 30, 348–49.
Ebling, F. J. G. (1970). Sebaceous glands. In:An Introduction to the Biology of the Skin (eds.) R. H. Champion, T. Gillman, A. J. Rook & R. T. Sims, pp. 184–196. Oxford and Edinburgh, Blackwells.
Flatt, J. P. &Ball, E. G. (1966). Studies on the metabolism of adipose tissue. XIX. An evaluation of the major pathways of glucose catabolism as influenced by acetate in the presence of insulin.J. biol. Chem. 241, 2862–69.
Geyer, G. (1973).Ultrahistochemie. Histochemische Arbeitsvorschriften für die Elektronenmikroskopie. 2nd Edn. Stuttgart: Fischer.
Glenner, G. G., Burtner, H. J. &Brown, G. W. (1957). The histochemical demonstration of monoamine oxidase activity by tetrazolium salts.J. Histochem. Cytochem. 5, 591–600.
Falprin, K. M. &Ohkawara, A. (1966). Lactate production and lactate dehydrogenase in human epidermis.J. invest. Derm. 47, 222–6.
Hellmann, K. (1955). Cholinesterase and amine oxidase in the skin: a histochemical investigation.J. Physiol. Lond. 129, 454–63.
Im, M. J. C. (1965). Distribution of dehydrogenases in the skin of the Rhesus monkey (Macaca mulatta).J. Histochem. Cytochem. 13, 668–76.
Im, M. J. C. &Hoopes, J. E. (1974). Enzymes of carbohydrate metabolism in normal human sebaceous glands.J. invest. Derm. 62, 153–60.
Jenkinson, D. M. &Black Burn, P. S. (1968). The distribution of nerves, monoamine oxidase and cholinesterase in the skin of the cat and dog.Res. vet. Sci. 9, 521–8.
Katz, J., Landau, B. R. &Bartsch, G. E. (1966). The pentose cycle, triose phosphate isomerization, and lipogenesis, in rat adipose tissue.J. biol. Chem. 241, 727–40.
Kristensen, S. (1975). A study of skin diseases in dogs and cats. I. Histology of the hairy skin of dogs and cats.Nord. VetMed. 27, 593–603.
Lowenstein, J. M. (1961a). The pathway of hydrogen in biosyntheses. I. Experiments with glucose-1-H3 and lactate-2-H3.J. biol. Chem. 236, 1212–16.
Lowenstein, J. M. (1961b). The pathway of hydrogen in biosyntheses. II. Extramitochondrial isocitrate dehydrogenase.J. biol. Chem. 236, 1217–19.
Meyer, W. &Neurand, K. (1976a). The distribution of enzymes in the skin of the domestic pig.Lab. Animals 10, 237–47.
Meyer, W. &Neurand, K. (1976b). Hydrolytic enzymes in the sebaceous glands of the domestic cat.Histochem. J. 8, 639–46.
Montagna, W. (1963). Comparative aspects of sebaceous glands. In:Advances in Biology of Skin, Vol. 4: The Sebaceous Glands (eds. W. Montagna, R. A. Ellis & A. F. Silver), pp. 32–45. Oxford: Pergamon Press.
Montagna, W. &Ellis, R. A. (1962). Histochemistry of enzymes in the skin. In:Handbuch der Histochemie, Vol. 7, part 2 (eds W. Graumann & K. Neumann), pp. 39–72. Stuttgart: Fischer.
Nachlas, M. M., Tsou, K. C., Cheg, C. S. &Seligman, A. M. (1957). Cytological demonstration of succinic dehydrogenase by use of a newp-nitrophenol substituted ditetrazole.J. Histochem. Cytochem. 5, 420–36.
Neurand, K. &Meyer, W. (1976). Enzymhistochemische Untersuchungen an den Talgdrüsen des Hausschweines.Anat. Anz. 140, 286–93.
Pearse, A. G. E. (1972).Histochemistry. Theoretical and Applied. 3rd Edn., Vol. 2. Edinburgh and London: Churchill-Livingstone.
Reiss, J. (1967). Der cytochemische Nachweis von Cytochromoxydase und Peroxydasen bei Pilzen.Histochemie 9, 281–99.
Rogdakis, E. (1972). Untersuchungen über die Aktivität von NADP-abhängigen Dehydrogenasen im Fettgewebe des Schweines als Index für den Fettansatz.Diss. Hohenheim.
Scarpelli, D. G., Hess, R. &Pearse, A. G. E. (1958). The cytochemical localization of oxidative enzymes. I. Diphosphopyridine nucleotide diaphorase.J. biophys. biochem. Cytol. 4, 747–51.
Scheving, L. E., Harrison, W. H. &Pauly, J. E. (1968). Daily fluctuation (circadian) in levels of epinephrine in the rat suprarenal gland.Am. J. Physiol. 215, 709–802.
Strauss, J. S. &Pochi, P. E. (1968). Histology, histochemistry., and electron microscopy of sebaceous glands in man. In:Handbuch der Haut-und Geschlechtskrankheiten, Ergänzungswerk Vol. 1, Part 1 (ed. J. Jadassohn) pp. 184–223. Berlin, Heidelberg, New York: Springer.
Wheatley, V. R. (1974). Cutaneous lipogenesis. Major pathways of carbon flow and possible interrelationships between the epidermis and sebaceous glands.J. invest. Derm. 62, 245–56.
Ziboh, V. A., Dreize, M. A. &Hsia, S. L. (1970). Inhibition of lipid synthesis and glucose-6-phosphate dehydrogenase in rat skin by dehydroepiandrosterone.J. Lipid Res. 11, 346–54.
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Meyer, W., Neurand, K. Oxidative enzymes in the sebaceous glands of the domestic cat. Histochem J 9, 309–316 (1977). https://doi.org/10.1007/BF01004767
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DOI: https://doi.org/10.1007/BF01004767