The Histochemical Journal

, Volume 9, Issue 3, pp 285–291 | Cite as

The Haematoxylin-basic Fuchsin-picric acid staining reaction as a test of myocardial viability in resuscitated and preserved hearts

  • D. K. C. Cooper


In the field of the transplantation of organs, there is a great need for anin vitro test of viability which would confirm that the organ was capable of performing its normalin vivo functions. Such a test should ideally be simple, rapid and reproducible. Preliminary studies using the Haematoxylin-Basic Fuchsin-picric acid (HBFP) staining reaction to assess myocardial ischaemia in resuscitated and preserved hearts would suggest that this test meets many of the requirements of a viability assay.

The test has been employed in hearts which have been in a state of anoxic arrest for 30 min and then resuscitated and preserved as an autoperfusing heart-lung preparation. The ‘positive’ response after 30 min anoxic arrest reverts to a ‘negative’ response after 2 h myocardial perfusion. In hearts which have been preserved as an autoperfusing heart-lung preparation with no interim period of anoxic arrest the HBFP stain response remains ‘negative’ throughout, confirming satisfactory myocardial perfusion.


Myocardial Perfusion Myocardial Ischaemia Myocardial Viability Anin Interim Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Chapman and Hall Ltd 1977

Authors and Affiliations

  • D. K. C. Cooper
    • 1
  1. 1.Department of Surgery, Cardiothoracic InstituteUniversity of LondonUK

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