The Histochemical Journal

, Volume 9, Issue 3, pp 285–291 | Cite as

The Haematoxylin-basic Fuchsin-picric acid staining reaction as a test of myocardial viability in resuscitated and preserved hearts

  • D. K. C. Cooper
Article

Synopsis

In the field of the transplantation of organs, there is a great need for anin vitro test of viability which would confirm that the organ was capable of performing its normalin vivo functions. Such a test should ideally be simple, rapid and reproducible. Preliminary studies using the Haematoxylin-Basic Fuchsin-picric acid (HBFP) staining reaction to assess myocardial ischaemia in resuscitated and preserved hearts would suggest that this test meets many of the requirements of a viability assay.

The test has been employed in hearts which have been in a state of anoxic arrest for 30 min and then resuscitated and preserved as an autoperfusing heart-lung preparation. The ‘positive’ response after 30 min anoxic arrest reverts to a ‘negative’ response after 2 h myocardial perfusion. In hearts which have been preserved as an autoperfusing heart-lung preparation with no interim period of anoxic arrest the HBFP stain response remains ‘negative’ throughout, confirming satisfactory myocardial perfusion.

Keywords

Myocardial Perfusion Myocardial Ischaemia Myocardial Viability Anin Interim Period 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bbott, W. M. (1969). Viability assays as applied to the cryopreservation of hearts and kidneys.Cryobiology 5, 454–62.Google Scholar
  2. Buja, L. M., Levitsky, S., Ferrans, V. J., Souther, S. G., Roberts, W. C. &Morrow, A. G. (1971). Acute and chronic effects of normothermic anoxia on canine hearts: light and electron microscopic evaluation.Circulation Suppl. 1 to Vols. 43 & 44, 44–50.Google Scholar
  3. Cohen, B. E. &Folkman, M. J. (1968). Cell death and the measurement of oxygen consumption of the isolated perfused organ. In:Organ Perfusion and Preservation, (ed. J. C. Norman), pp. 471–86. New York: Appleton-Century-Crofts Inc.Google Scholar
  4. Cooper, D. K. C. (1974). Studies on resuscitation and short-term preservation of the canine heart. Ph.D. Thesis, University of London.Google Scholar
  5. Cooper, D. K. C. (1975). A simple method of resuscitation and short-term preservation of the donor heart for transplantation.J. thorac. cardiovasc. Surg. 70, 896–908.Google Scholar
  6. Copeland, J., Kosek, J. C. &Hurley, E. J. (1968). Early functional and ultrastructural recovery of canine cadaver hearts.Circulation Suppl. 2 to Vols 37 & 38, 188–200.Google Scholar
  7. Couch, N. P. &Middleton, M. K. (1968). Effect of storage temperature on the electrometric surface ion activity of ischemic liver and heart.Surgery 64, 1099–105.Google Scholar
  8. Delage, C., Mullick, F. G. &Irey, N. S. (1973). Myocardial lesions in anaphylaxis: a histochemical study.Archs. Path. 95, 185–9.Google Scholar
  9. Demikhov, V. P. (1962).Experimental transplantation of vital organs (trans. B. Haigh). New York: Consultants Bureau.Google Scholar
  10. Gamble, W. J., Stark, J., Lafarge, C. G. &Monroe, R. G. (1972). Ventricular performance in the isolated supported dog heart observed over a period of 24 hours.J. thorac. cardiovasc. Surg. 63, 906–16.Google Scholar
  11. Holdefer, W. F. &Edwards, W. S. (1968). Ventricular performance characteristics in a metabolically supported isolated heart preparation: a model for evaluating cardiac storage methods.Surg. Forum 14, 225–7.Google Scholar
  12. Levitsky, S., Williams, W. H., Detmer, D. E., Mcintosh, C. L. &Morrow, A. G. (1970). A functional evaluation of the preserved heart.J. thorac. cardiovasc. Surg. 60, 625–35.Google Scholar
  13. Lie, J. T., Holley, K. E., Kampa, W. R. &Titus, J. L. (1971). New histochemical method for morphological diagnosis of early stages of myocardial ischemia.Mayo Clinic Proc. 46, 319–27.Google Scholar
  14. Longmore, D. B., Cooper, D. K. C., Hall, R. W., Sekabunga, J., &Welch, W. (1969). Transplantation of the heart and both lungs. II: Experimental cardiopulmonary transplantation.Thorax 24, 391–8.Google Scholar
  15. Maginn, R. R. (1968). A rapid test for tissue viability prior to transplantation.Br. J. Surg. 55, 15–17.Google Scholar
  16. Maginn, R. R. &Hadjimichalis, E. (1968). Pretransplant cardiac viability assays. In:Organ Perfusion and Preservation. (ed. J. C. Norman), pp. 505–12. New York: Appleton-Century-Crofts, Inc.Google Scholar
  17. Mundth, E. D., Sokol, D. M., Levine, F. H. &Austen, W. G. (1969). Preservation of myocardial function during extended periods of coronary ischemia.Surg. Forum 20, 176–8.Google Scholar
  18. Pitzele, S., Sze, S. &Dobell, A. R. C. (1971). Functional evaluation of the heart after storage under hypothermic coronary perfusion.Surgery 70, 569–77.Google Scholar
  19. Proctor, E. &Parker, R. (1968). Preservation of the isoalted heart for 72 hours.Br. med. J. 4, 296–8.Google Scholar
  20. Reis, R. L., Staroscik, R. N., Rodgers, B. M., Enright, L. P. &Morrow, A. G. (1969). Left ventricular function after ischemic cardioplegia.Archs Surg. 99, 815–20.Google Scholar
  21. Robicsek, F., Lesage, A., Sanger, P. W., Daugherty, H. K. Gallucci, V. &Bagby, E. (1967). Transplantation of ‘live’ hearts.Am. J. Cardiol. 20, 803–11.Google Scholar
  22. Scherer, A. T. &Masi, A. T. (1975). Technical aspects of the Haematoxylin-Basic Fuchsin-picric acid (HBFP) stain applied to skeletal muscle.Histochem. J. 7, 335–41.Google Scholar
  23. Terasaki, P. I., Martin, D. C. &Smith, R. B. (1967). A rapid metabolism test to screen cadaver kidneys for transplantation.Transplantation 5, 76–8.Google Scholar
  24. Webb, W. R. &Howard, H. S. (1957). Cardiopulmonary transplantation.Surg. Forum. 8, 313–17.Google Scholar

Copyright information

© Chapman and Hall Ltd 1977

Authors and Affiliations

  • D. K. C. Cooper
    • 1
  1. 1.Department of Surgery, Cardiothoracic InstituteUniversity of LondonUK

Personalised recommendations