Summary
The metaplastic polyp is a non-neoplastic epithelial lesion found within the human colorectum. Although not regarded as precancerous, recent studies have demonstrated the expression of multiple cancerassociated phenotypes. This might indicate a possible indirect relationship between metaplastic polyps and colorectal cancer. Epithelial secretory component and IgA were demonstrated by the immunoperoxidase technique and staining intensities were assessed semiquantitatively. The findings were related to cellular differentiation in normal colorectal epithelium as compared to the metaplastic polyp. The crypt base cells and also the surface epithelial cells stained with similar intensity in both types of epithelium. However, the expected increase in staining characterizing normal lower and upper crypt columnar cells and reduction in staining associated with the switch from crypt to surface columnar cell was not observed in the metaplastic polyp. Metaplastic crypt columnar cells showed significantly reduced staining for both IgA and secretory component as compared to their normal counterparts. There was also a significant reduction in the number of IgA-secreting plasma cells in the lamina propria of the metaplastic polyp. These findings are consistent with the concept of a premature switch to mature surface cell characteristics within the metaplastic polyp. They are discussed in the light of other changes in phenotype associated with this lesion.
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References
Ahnen, D. J., Nakane, P. K. &Brown, W. R. (1982) Ultrastructural localization of carcinoembryonic antigen in normal intestine and colon cancer.Cancer 49, 2077–90.
Boland, C. R., Montgomery, C. K. &Kim, Y. S. (1982) A cancer-associated mucin alteration in benign colonic polyps.Gastroenterology 82, 664–72.
Brandtzaeg, P. (1981) Transport models for secretory IgA and secretory IgM.Clin. exp. Immun. 44, 221–33.
Brown, D. T., Anderton, B. H. &Wylie, C. C. (1983) The organisation of intermediate filaments in normal human colonic epithelium and colonic carcinoma cells.Int. J. Cancer 32, 163–9.
Brown, W. R., Isobe, Y. &Nakane, P. (1976) Studies on translocation of immunoglobulins across intestinal epithelium.Gastroenterology 71, 985–95.
Czernobilsky, B. &Tsou, K. C. (1968) Adenocarcinoma, adenomas and polyps of the colon.Cancer 21, 165–77.
Dexter, D. L., Lev, R., McKendall, G. R., Mitchell, P. &Calabres, P. (1984) Sodium butyrate-induced alteration of growth properties and glycogen levels in cultured human colon carcinoma cells.Histochem. J. 16, 137–49.
Hager, J. C., Gold, D. V., Barbosa, J. A., Fligiel, Z., Miller, F. &Dexter, D. L. (1980) N-Dimethylformamide-induced modulation of organ and tumour-associated markers in cultured human colon carcinoma cells.J. natn. Cancer Insti. 64, 439–46.
Hayashi, T., Yatani, R., Apostaol, J. &Stemmenmann, G. N. (1974) Pathogenesis of hyperplastic polyps of the colon: A hypothesis based on ultrastructural and in vitro cell kinetics.Gastroenterology 66, 347–56.
Isaacson, P. (1982a) Immunoperoxidase study of the secretory immunoglobulin system in colonic neoplasia.J. clin. Path. 34, 14–25.
Isaacson, P. (1982b) Immunoperoxidase study of the secretory immunoglobulin system and lysozome in normal and diseased gastric mucosa.Gut 23, 578–88.
Jass, J. R. (1983) Relation between metaplastic polyp and carcinoma of the colorectum.Lancet i, 28–30.
Jass, J. R., Filipe, M. I., Abbas, S., Falcon, C. A. J., Wilson, Y. &Lovell, D. (1984a) A morphological and histochemical study of metaplastic polyps of the colorectum.Cancer 53, 510–5.
Jass, J. R., Strudley, I. & Faludy, J. (1984b) Histochemistry of epithelial metaplasia and dysplasia in human stomach and colorectum.Scand. J. Gastroenterol. (in press).
Kaye, G. I., Fenoglio, C. M., Pascal, R. R. &Lane, N. (1973) Comparative electron microscopic features of normal, hyperplastic and adenomatous human colonic epithelium.Gastroenterology 64, 926–45.
Kim, Y. S., Tsao, D., Siddiqui, B., Whitehead, J. S., Arnstein, P., Bennett, J. &Hicks, J. (1980) Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells.Cancer 45, 1185–92.
Mepham, B. L., Frater, W. &Mitchell, B. S. (1979) The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique.Histochem. J. 11, 345–57.
Parrott, D. M. V. (1976) The gut as a lymphoid organ.Clin. Gastroenterol. 5, 211–28.
Rognum, T. O., Fausa, O. &Brandtzaeg, P. (1982) Immunohistochemical evaluation of carcinoembryonic antigen, secretory component and epithelial IgA in tubular and villous large-bowel adenomas with different grades of dysplasia.Scand. J. Gastroenterol. 17, 341–8.
Spandidos, D. A. &Kerr, I. B. (1984) Elevated expression of the human ras oncogene family in premalignant and malignant tumours of the colorectum.Br. J. Cancer 49, 681–8.
Sternberger, L. A., Hardy, P. H. J., Cuculis, J. J. &Meyer, H. G. (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti horseradish peroxidase) and its use in the identification of spirochaetes.J. Histochem. Cytochem. 18, 325–33.
Von Bassewitz, D. B. &Grundmann, E. (1983) Intermediate-sized filaments in cells of normal human colon mucosa, adenomas and carcinomas.J. exp. clin. Cancer Res. Suppl. 2, 28.
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Jass, J.R., Faludy, J. Immunohistochemical demonstration of IgA and secretory component in relation to epithelial cell differentiation in normal colorectal mucosa and metaplastic polyp: a semiquantitative study. Histochem J 17, 373–380 (1985). https://doi.org/10.1007/BF01004598
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DOI: https://doi.org/10.1007/BF01004598