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An ultrastructural study of complex carbohydrates in the posterior chamber and vitreous base of the mouse

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Summary

The fibrillar and mucoid extracellular matrix of the posterior chamber and vitreous base was studied in the mouse by electron microscopy using fixation and staining methods that demonstrated complex carbohydrates. These methods, including block-staining with Alcian Blue, allowed globular and filamentous hyaluronic acid, finely filamentous oligosaccharides, laminated glycolipids or lipophilic glycoproteins and stellate proteoglycan monomers to be identified tentatively. There was much less globular hyaluronic acid along the basement membrane of the peripheral retina and ciliary body than has been observed in the posterior fundus. A finely filamentous network on the basement membrane interconnected with a similar network covering individual collagen fibrils, zonules and meridional fibrillar laminae as well as with a branching fibrillar network that was seen in the posterior chamber and vitreous base. This interconnected system of fibrillar proteins and complex carbohydrates was also connected to the anterior hyaloid membrane. The infoldings of the ciliary epithelium contained stellage densities with characteristics of proteoglycan monomers similar to those reported in the matrix of cartilage. The complex carbohydrates of the posterior chamber and vitreous base are of several types known to affect protein function, provide water binding and assist in mechanical stability.

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  1. Department of Pathology, University of Southern California, 2011 Zonal Avenue, 90033, Los Angeles, California, USA

    Roy H. Rhodes

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Rhodes, R.H. An ultrastructural study of complex carbohydrates in the posterior chamber and vitreous base of the mouse. Histochem J 17, 291–312 (1985). https://doi.org/10.1007/BF01004592

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