The Histochemical Journal

, Volume 15, Issue 11, pp 1141–1150 | Cite as

Cytochemical evidence for functional zonation of parietal cells within the gastric glands of the mouse

  • Gary R. Coulton
  • J. Anthony Firth
Papers

Summary

Parietal cells in the luminal segments of mouse gastric glands show high activity of acid-secreting potassium-dependent adenosine triphosphatase (H+, K+-ATPase) and of nicotinamide adenine dinucleotide-linked isocitrate dehydrogenase (NAD-ICDHase) and malate dehydrogenase (MDHase) but low activity of succinate dehydrogenase (SDHase). This pattern of activity is reversed in the basal segments of the same glands. These results and previous morphological findings support the conclusion that luminal segment parietal cells are much more active in hydrochloric acid secretion than those of the basal segment. The origin of this zonation may be either cellular deterioration with age or some more specific form of regulation of parietal cell metabolism.

Keywords

Adenine Succinate Nicotinamide Acid Secretion Isocitrate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Coulton, G. R. &Firth, J. A. (1982a) Modification by feeding regime of the intensity and distribution of cytochemically localized proton trassport adenosine triphosphatase activity in mouse stomach.J. Physiol. 326, 26–7P.Google Scholar
  2. Coulton, G. R. &Firth, J. A. (1982b) Differences in histochemical properties of parietal glands in the upper and lower segments of mouse gastric glands.J. Anat. 135, 823–4.Google Scholar
  3. Dibona, D. R., Schafer, J. A., Berglindh, T. A. &Sachs, G. (1981) The use of combined differential interference-contrast and fluorescence optics for analysis of epithelial transport. InEpithelial Ion and Water Transport (edited byMacknight, A. D. C. andLeader, J. P.), pp. 1–17. New York: Raven Press.Google Scholar
  4. Firth, J. A. &Stranks, G. J. (1981a) Gastric proton pump localization. Application of triphosphatase and monophosphatase techniques.J. Histochem. Cytochem. 29, 344–50.Google Scholar
  5. Firth, J. A. &Stranks, G. J. (1981b) Localization of recently characterized membrane transport adenosine triphosphatases.Histochem. J. 13, 517–24.Google Scholar
  6. Forte, J. G. &Lee, H. C. (1977) Gastric adenosine triphosphatases: a review of their possible role in HCl secretion.Gastroenterology 73, 921–6.Google Scholar
  7. Forte, T. M., Machen, T. E. &Forte, J. G. (1977) Ultrastructural changes in oxyntic cells associated with secretory function: a membrane-recycling hypothesis.Gastroenterology 73, 941–55.Google Scholar
  8. Helander, H. F. (1976) Stereological changes in rat parietal cells after vagotomy and antrectomy.Gastroenterology 71, 1010–8.Google Scholar
  9. Helander, H. F. &Hirschowitz, B. I. (1972) Quantitative ultrastructural studies on gastric parietal cells.Gastroenterology 63, 951–61.Google Scholar
  10. Ito, S. &Schofield, G. C. (1974) Studies on the depletion and accumulation of microvilli and changes in the tubulovesicular department of mouse parietal cells in relation to gastric acid secretion.J. Cell Biol. 63, 364–82.Google Scholar
  11. Jacobs, D. M. &Sturtevant, R. P. (1982) Circadian ultrastructural changes in rat gastric parietal cells under altered feeding regimens: a morphometric study.Anat. Rec. 203, 101–13.Google Scholar
  12. Lawn, A. M. (1960) Observations on the fine structure of the gastric parietal cell of the rat.J. biophys. biochem. Cytol. 7, 161–77.Google Scholar
  13. Lehninger, A. L. (1975)Biochemistry, 2nd edn. pp. 459–60. New York: Worth.Google Scholar
  14. Pearse, A. G. E. (1972)Histochemistry: Theoretical and applied, Vol. 2, 3rd edn, pp. 1342–4. Edinburgh, London, New York: Churchill-Livingstone.Google Scholar
  15. Ragins, H., Wincze, F., Liu, S. M. &Dittenbrenner, M. (1968) The origin and survival of gastric parietal cells in the mouse.Anat. Rec. 162, 99–110.Google Scholar
  16. Saccomani, G., Helander, H. F., Crago, S., Chang, H. H., Dailey, D. W. &Sachs, G. (1979) Characterization of gastric mucosal membranes X. Immunological studies of gastric (H++K+)-ATPase.J. Cell Biol. 83, 271–83.Google Scholar
  17. Soll, A. H. (1980) Secretogogue stimulation of14C-aminopyrine accumulation by isolated mammalian parietal cells.Am. J. Physiol. 238, G366–75.Google Scholar

Copyright information

© Chapman and Hall Ltd 1983

Authors and Affiliations

  • Gary R. Coulton
    • 1
  • J. Anthony Firth
    • 1
  1. 1.Department of AnatomySt. George's Hospital Medical SchoolLondonUK

Personalised recommendations