Skip to main content
SpringerLink
Log in

Cytochemical studies on the localization of alkaline phosphatase and HCO −3 -activated adenosine triphosphatase in the brush border membrane of rat duodenal enterocytes

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

Cytochemical techniques were used to demonstrate, with appropriate controls, alkaline phosphatase and HCO −3 -activated adenosine triphosphatase (ATPase) in rat duodenal brush border microvillus membranes. Intense activity of ecto-alkaline phosphatase activity was demonstrated with 2-glycerophosphate as substrate. Although biochemical assays suggested thatl-phenylalanine inhibited both alkaline phosphatase and HCO −3 -activated ATPase, cytochemical studies indicated that there was marked inhibition of alkaline phosphatase revealing a specific HCO −3 -activated ATPase on the inner aspect of the microvillus membrane. While it is tempting to suggest that this HCO −3 -activated ATPase is implicated in active bicarbonate secretion by the duodenum, decisive identification is not yet possible.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boyd, C. A. R. &Chipperfield, A. R. (1980) Are alkaline phosphatase and bicarbonate dependent ATPase the same enzyme?J. Physiol., Lond. 303, 63.

    Google Scholar 

  • Busse, D., Pohl, B., Bartel, H. &Buschmann, F. (1980) The Mg2+ dependent adenosine triphosphatase activity in the brush border of rabbit kidney cortex.Archs. Biochem. Biophys. 201, 147–59.

    Google Scholar 

  • Fishman, W. H. &Sie, H.-G. (1971) Organ-specific inhibition of human alkaline phosphatase isoenzymes of liver, bone, intestine and placenta;l-phenylalanine,l-tryptophan andl-homoarginine.Enzymologia 41, 141–67.

    Google Scholar 

  • Flemstrom, G. &Garner, A. (1982) Gastroduodenal HCO −3 transport: characteristics and proposed role in acidity, regulation and mucosal protection.Am. J. Physiol. 242, 183–93.

    Google Scholar 

  • Fosset, M., Chappelet-Tordo, D. &Lazdunski, M. (1974) Intestinal alkaline phosphatase. Physical properties and quarternary structure.Biochemistry 13, 1783–8.

    Google Scholar 

  • Humphreys, M. H. &Chou, L. Y. N. (1979) Anion-stimulated ATPase activity of brush border from rat small intestine.Am. J. Physiol. 236, 70–6.

    Google Scholar 

  • Humphreys, M. H., Kaysen, G. A., Chou, L. Y. &Watson, J. B. (1980) Anion-stimulated phosphohydrolase activity of intestinal alkaline phosphatase.Am. J. Physiol. 238, 3–9.

    Google Scholar 

  • Kaysen, O. A., Chou, L. Y. &Humphreys, M. H. (1979) Requirement of Zn to demonstrate HCO −3 -stimulated ATPase activity of rat small intestinal brush border.J. Cell Biol. 82, 780–2.

    Google Scholar 

  • Kessler, M., Aguto, O., Storelli, C., Murer, H., Muller, M., Muller, H. &Semenza, G. (1975) A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes.Biochim. biophys. Acta 506, 136–54.

    Google Scholar 

  • Koenig, C. &Vial, J. D. (1970) A histochemical study of adenosine triphosphatase in the toad (Bufo Epinulosus) gastric mucosa.J. Histochem. Cytochem. 18, 340–53.

    Google Scholar 

  • Peters, T. J. (1976) Analytical subcellular fractionation of jejunal biopsy specimens: methodology and characterisation of the organelles in normal tissue.Clin. Sci. Mol. Med. 51, 557–74.

    Google Scholar 

  • Peters, T. J. &Shio, H. (1976) Subcellular fractionation studies on isolated rat jejunal enterocytes with special reference to the separation of lysosomes, peroxisomes and mitochondria.Clin. Sci. Mol. Med. 50, 355–66.

    Google Scholar 

  • Sachs, G., Spenney, J. G. &Lewin, M. (1978) H+ transport: regulation and mechanism in gastric mucosa and membrane vesicles.Physiol. Rev. 58, 106–73.

    Google Scholar 

  • Simson, J. N. L., Merhav, A. &Silen, W. (1981) Alkaline secretion by amphibian duodenum. I. General characteristics.Am. J. Physiol. 240, 401–8.

    Google Scholar 

  • Stekhoven, F. S. &Bonting, S. L. (1981) Transport adenosine triphosphatases: properties and functions.Physiol. Rev. 61, 1–76.

    Google Scholar 

  • Stiel, D., Murray, D. J. &Peters, T. J. (1983) Mucosal enzyme activities, with special reference to enzymes implicated in bicarbonate secretion, in the duodenum of rats with cysteamine-induced ulcers.Clin. Sci. 64, 341–7.

    Google Scholar 

  • Van Amelsvoort, J. M. M., De Pont, J. J. H. H. M., Stols, A. L. H. &Bonting, S. L. (1977) Is there a plasma membrane-located anion-sensitive ATPase? II. Further studies on rabbit kidney.Biochim. biophys. Acta 471, 78–91.

    Google Scholar 

  • Yurkiv, V. A., Shubin, V. S. &Duvakin, I. A. (1982) Adenosine triphosphatase activity in rabbit small intestine as influenced by mono- and bivalent cations and anions.Biochem. Internat. 4, 67–74.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK

    Gaynor Sharp, D. Stiel & T. J. Peters

Authors
  1. Gaynor Sharp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Stiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. J. Peters
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharp, G., Stiel, D. & Peters, T.J. Cytochemical studies on the localization of alkaline phosphatase and HCO −3 -activated adenosine triphosphatase in the brush border membrane of rat duodenal enterocytes. Histochem J 15, 1131–1139 (1983). https://doi.org/10.1007/BF01003976

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01003976

Keywords

Search

Navigation