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The Histochemical Journal

, Volume 15, Issue 11, pp 1123–1130 | Cite as

Localization of lanthanum tracer in oral epithelium using transmission electron microscopy and the electron microprobe

  • Christopher Squier
  • John Edie
Papers

Summary

Although water soluble tracers have been used to localize intercellulr permeability barriers with the transmission electron microscope, there is the possibility of translocation or loss of the tracer during processing. This study compares the localization of lanthanum tracer in keratinized oral epithelium after routine processing with lanthanum seen after using freeze drying to avoid aqueous fixation and dehydration. The electron probe was used to identify the lanthanum tracer in the tissue and to distinguish it from other electron-dense material. After incubating small biopsies of rat palate mucosa in 1% lanthanum nitrate, specimens were either routinely processed for electron microscopy or quick frozen, dehydrated, fixed in osmium vapour and infiltrated with epoxy resin. Examination in the transmission electron microscope indicated that preservation of the freeze dried tissue did not compare favourably with that of normally processed tissue, but the distribution of the electron-dense tracer in the intercellular spaces and the extent of the penetration through the epithelia was similar in the two types of preparations. Confirmation of the tracer as lanthanum was obtained by wavelength dispersive X-ray analysis with the electron probe. The results indicate that no appreciable shift in the localization of the tracer is introduced by routine aqueous fixation and dehydration for electron microscopic examination.

Keywords

Lanthanum Electron Probe Permeability Barrier Electron Microscopic Examination Oral Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barnett, M. L. &Szabo, G. (1973) Gap junctions in human gingival keratinized epithelium.J. Periodontol. Res. 8, 117–26.Google Scholar
  2. Edelmann, L. (1978) A simple freeze-drying technique for preparing biological tissue without chemical fixation for electron microscopy.J. Microscopy 112, 243–8.Google Scholar
  3. Elias, P. M. (1981) Lipids and the epidermal permeability barrier.Arch. Derm. Res. 270, 95–117.Google Scholar
  4. Elias, P. M. &Friend, D. S. (1975) The permeability barrier in mammalian epidermis.J. Cell Biol. 65, 180–91.Google Scholar
  5. Elias, P. M. &Nemanic, M. K. (1980)In situ precipitation: a novel cytochemical technique for visualization of permeability pathways in mammalian stratum corneum.J. Histochem. Cytochem. 28, 513–78.Google Scholar
  6. Gupta, B. L. &Hall, T. A. (1979) Quantitative electron probe X-ray microanalysis of electrolyte elements within epithelial tissue compartments.Fedn. Proc. Fedn. Am. Socs exp. 38, 144–53.Google Scholar
  7. Hashimoto, K. (1971) Intercellular spaces of the human epidermis as demonstrated with lanthanum.J. invest Derm. 57, 17–31.Google Scholar
  8. Henrikson, R. C. &Stacy, B. D. (1971) The barrier to diffusion across ruminal epithelium: a study by electron microscopy using horseradish peroxidase, lanthanum, and ferritin.J. Ultrastruct. Res. 34, 72–82.Google Scholar
  9. Ingram, F. D. &Ingram, M. J. (1975) Quantitative analysis with the freeze-dried, plastic embedded tissue specimen.J. Microscopie Biol. Cell. 22, 193–204.Google Scholar
  10. Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy.J. Cell Biol. 35, 213.Google Scholar
  11. King, C. S., Moore, N., Marks, R. &Nicholls, S. (1978) Preliminary studies into percorneal penetration and elemental content of the stratum corneum using X-ray microanalysis.Arch. Derm. Forsch. 263, 257–65.Google Scholar
  12. Komura, J., Shimada, Y. &Ofuji, S. (1969) Lanthanum staining of the epidermal intercellular spaces: An electron microscopic study.Arch. klin. exp. Derm. 235, 221–7.Google Scholar
  13. Machen, T. E., Erlij, D. &Wooding, F. B. (1972) Permeable junctional complexes. The movement of lanthanum across rabbit gall bladder and intestine.J. Cell Biol. 54, 302–12.Google Scholar
  14. Mahrle, G. (1977) Intramembraneous particles and transmembraneous ionic channels in the epidermal cell membrane.Arch. Derm. Res. 260, 143–51.Google Scholar
  15. Martinez-Palomo, A. (1970) Ultrastructural modifications of intercellular junctions in some epithelial tumors.Lab. Invest. 22, 605–14.Google Scholar
  16. Martinez-Palomo, A., Erlij, D. &Bracho, H. (1971) Localization of permeability barriers in the frog skin epithelium.J. Cell Biol. 50, 277–87.Google Scholar
  17. Orwin, D. F. G., Thomson, R. W. &Flower, N. E. (1973) Plasma membrane differentiations of keratinizing cells of the wool follicle. I-IV.J. Ultrastruct. Res. 45, 1–49.Google Scholar
  18. Revel, J. P. &Karnovsky, M. J. (1967) Hexagonal array of subunits in intercellular junctions of the mouse heart and liver.J. Cell Biol. 33, 7–12.Google Scholar
  19. Schatzki, P. F. &Newsome, A. (1975) Neutralized lanthanum solution: a largely noncolloidal ultrastructural tracer.Stain Technol. 50, 171–8.Google Scholar
  20. Shea, S. M. (1971) Lanthanum staining of the surface coat of cells.J. Cell Biol. 51, 611–20.Google Scholar
  21. Squier, C. A. (1973) The permeability of keratinized and non-keratinized oral epithelium to horseradish peroxidase.J. Ultrastruct. Res. 43, 160–77.Google Scholar
  22. Squier, C. A. &Hopps, R. M. (1977) The chemical nature of the epithelial permeability barrier in oral mucosa.J. dent. Res. 56, 112.Google Scholar
  23. Squier, C. A. &Rooney, L. (1976) The permeability of keratinized and nonkeratinized oral epithelium to lanthanumin vivo.J. Ultrastruct. Res. 54, 286–95.Google Scholar
  24. Wolff, K. &Schreiner, E. (1968) An electron microscopic study on the extraneous coat of keratinocytes and the intercellular space of the epidermis.J. invest. Derm. 51, 418–30.Google Scholar

Copyright information

© Chapman and Hall Ltd 1983

Authors and Affiliations

  • Christopher Squier
    • 1
  • John Edie
    • 1
  1. 1.Dows Institute for Dental Research, College of DentistryThe University of IowaIowa CityUSA

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