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A quantitative microspectrophotometric study of the lead precipitation reaction for the histochemical demonstration of acid phosphatase

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Synopsis

The possibility of quantitating microspectrophotometrically the lead precipitation reaction for the histochemical demonstration of acid phosphatase was studied using the following models: sections of rat kidney and human prostate gland, and polyacrylamide films with incorporated prostate gland homogenate. The activity of the polyacrylamide models was previously evaluated by a biochemical method and the specificity of the reaction was controlled with specific inhibitors. Linear relations between cytophotometric readings and enzymatic activity, and between optical densities and different incubation periods, were demonstrated. The results obtained indicate the validity of the microspectrophotometric quantitation for this reaction with direct readings over a wide range of the visible light spectrum.

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References

  • Abrahamson, D. E., Rigatuso, J. L. &Lazorow, A. (1969). Cytophotometric quantitation of G1-6-phosphatase activity in rat liver.J. Histochem. Cytochem. 17, 107–9.

    Google Scholar 

  • Andersch, M. &Szczpinski, A. (1947). Use ofp-nitrophynylphosphate as the substrate in determination of serum acid phosphatase.Am. J. clin. Path. 17, 571–6.

    Google Scholar 

  • Barka, T. &Anderson, P. J. (1963).Histochemistry. Theory, Practice and Bibliography, pp. 211–47. New York, Evanston, London: Hoeber Medical Division, Harpe & Row.

    Google Scholar 

  • Cabrini, R. L., Itoiz, M. E., Mayo, J., Smolko, E. E. &Klein-Szanto, A. J. P. (1970). Microspectrophotometric study of histoenzymic reactions in rat epidermis subjected to 0.5 Mrad of deuteron irradiation.Int. J. radiat. Biol. 18, 415–21

    Google Scholar 

  • Cabrini, R. L., Viuñales, E. J. &Itoiz, M. E. (1969). A microspectrophotometric method for histochemical quantitation of succinic dehydrogenase.Acta Histochem. 34, 287–91.

    Google Scholar 

  • Duijn, P. Van, Pascoe, E. &Van Der Ploeg, M. (1967). Theoretical and experimental aspects of enzyme determination in a cytochemical model system of polyacrylamide films containing alkaline phosphatase.J. Histochem. Cytochem. 15, 631–45.

    Google Scholar 

  • Duijn, P. Van &Van Der Ploeg, M. (1972). The use of cellulose and polyacrylamide films as vehicles of compounds for model studies in quantitative cytophotometry and for calibration of results in biochemical units.Acta Histochem. Suppl. XII, 125–30.

    Google Scholar 

  • Gomori, G. (1950). An improved histochemical technique for acid phosphatase.Stain Technol. 25, 81–7.

    Google Scholar 

  • Itoiz, M. E., Carranza, F. A., Gimenez, I. B. &Cabrini, R. L. (1972). Microspectrophotometric analysis of succinic dehydrogenase and glucose-6-phosphate dehydrogenase in human oral and gingival epithelium.J. Periodot. Res. 7, 14–20.

    Google Scholar 

  • Jarret, A. &Please, N. W. (1970). A quantitative histochemical technique for the estimation of azo dye coupling reactions.Histochem. J. 2, 297–313.

    Google Scholar 

  • Klein-Szanto, A. J. P. &Cabrini, R. L. (1972). Acute response of oxidative enzyme systems in epidermis subjected to beta radiation.Arch. Derm. Forsch. 243, 226–31.

    Google Scholar 

  • Krestinskaia, T. B. &Manusova, N. B. (1969). Cytophotometric study of Na+ K+ activated Mg2+ dependent ATPase in rat kidney.Inf. Acad. Sci. URSS 187, 217–32.

    Google Scholar 

  • Lojda, Z., Van Der Ploeg, M. &Van Duijn, P. (1967). Phosphates of the naphthol AS acid series in the quantitative determination of alkaline and acid phosphatase activities “in situ” studied in polyacrylamide membrane model systems and by cytospectrophotometry.Histochemie 11, 13–32.

    Google Scholar 

  • Rearse, A. G. E. (1960).Histochemistry Theoretical and Applied, pp. 438–9. London: Churchill.

    Google Scholar 

  • Fis, H. &Mirsky, A. (1949). Quantitative cytochemical determination of DNA with the Feulgen nuclear reaction.J. gen. Physiol. 33, 125–46.

    Google Scholar 

  • Swift, M. (1950). The DNA content of animal nucleic acids.Physiol. Zoöl. 23, 169–98.

    Google Scholar 

  • Taljedal, I. B. (1969). Kinetics of glucose-6-phosphatase in pancreatic islets as revealed by staining histochemistry.Histochemie 19, 355–62.

    Google Scholar 

  • Taljedal, I. B. (1972). Quantitative histochemical aspects of glucose-6-phosphate hydrolysis and oxidation in the pancreatic islet β-cells.Acta Histochem. Suppl. XII, 101–10.

    Google Scholar 

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Authors and Affiliations

  1. Department of Radiobiology, National Atomic Energy Commission, Argentina

    R. L. Cabrini, A. C. C. Frasch & M. E. Itoiz

  2. Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, Argentina

    R. L. Cabrini, A. C. C. Frasch & M. E. Itoiz

Authors
  1. R. L. Cabrini
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  2. A. C. C. Frasch
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  3. M. E. Itoiz
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Cabrini, R.L., Frasch, A.C.C. & Itoiz, M.E. A quantitative microspectrophotometric study of the lead precipitation reaction for the histochemical demonstration of acid phosphatase. Histochem J 7, 419–426 (1975). https://doi.org/10.1007/BF01003879

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  • DOI: https://doi.org/10.1007/BF01003879

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