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Lactate dehydrogenase isoenzyme patterns in isolated cells of pig gastric mucosa

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A technique is described for obtaining a relatively pure suspension of parietal cells from pig gastric mucosa. All cell types were released from the tissue by treatment with pronase. Four cellular fractions were separated by centrifugation on a discontinuous Ficoll gradient. One fraction contained 68% (SD 13.3) parietal cells and revealed a high lactate dehydrogenase-1 level compared to the other fractions.

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Author notes

  1. B. G. Timms

    Present address: Tenovus Institute for Cancer Research, The Heath, Cardiff, UK

  2. B. Prochazka-Perthen

    Present address: Gesellschaft Fürstrahlen und Umweltforschung, Ingolstadter Landstrasse I, 8042, Neuherberg bei München

Authors and Affiliations

  1. Department of Biological Sciences, University of Aston in Birmingham, Gosta Green, Birmingham, UK

    B. G. Timms & B. Prochazka-Perthen

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  1. B. G. Timms
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  2. B. Prochazka-Perthen
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Timms, B.G., Prochazka-Perthen, B. Lactate dehydrogenase isoenzyme patterns in isolated cells of pig gastric mucosa. Histochem J 7, 401–409 (1975). https://doi.org/10.1007/BF01003877

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  • DOI: https://doi.org/10.1007/BF01003877

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