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Histochemistry of hydrolytic enzymes in resting submandibular glands of rabbits

The Histochemical Journal volume 17, pages 683–698 (1985)Cite this article

Summary

The distribution of several hydrolytic enzymes was investigated in rabbit submandibular glands at both the light and electron microscopical levels. Glands were fixed by either immersion or perfusion fixation with a variety of fixatives containing 1–2% glutaraldehyde and 2–4% formaldehyde in 0.1m cacodylate buffer at pH 7.2. Light microscopically, the acinar cells showed some staining for ATPase, acid phosphatase and nonspecific esterases but showed weak staining for thiamine pyrophosphatase. Acid phosphatase staining occurred most strongly in granular tubule cells. Staining for esteroproteases was confined to the periluminal rims of intercalary and striated ducts. Alkaline phosphatase was very sensitive to glutaraldehyde and was confined to myoepithelial cells.

Electron microscopical observations revealed the presence of acid phosphatase reaction product in lysosomes, immature granules and in GERL-like structures, the last being much more conspicuous in the granular tubule cells. ATPase reaction product was localized to the basal and luminal plasma membranes and lumina of both acinar and granular tubule cells. The Golgi complex of these two types of cells exhibited only moderate amounts of reaction product for thiamine pyrophosphatase. Alkaline phosphatase activity, on the other hand, was exclusively localized to myoepithelial cells in their plasma membranes and sometimes in the nuclear envelope.

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  1. Department of Oral Pathology, King's College School of Medicine and Dentistry, The Rayne Institute, SE5 9NU, London, UK

    K. Kyriacou & J. R. Garrett

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  1. K. Kyriacou
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  2. J. R. Garrett
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Kyriacou, K., Garrett, J.R. Histochemistry of hydrolytic enzymes in resting submandibular glands of rabbits. Histochem J 17, 683–698 (1985). https://doi.org/10.1007/BF01003520

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  • DOI: https://doi.org/10.1007/BF01003520

Keywords

  • Hydrolytic Enzyme
  • Acid Phosphatase
  • Acinar Cell
  • Alkaline Phosphatase Activity
  • Submandibular Gland