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The Histochemical Journal

, Volume 2, Issue 1, pp 29–34 | Cite as

Autoradiographic localization of [3H]5-hydroxytryptophan uptake by rat neuronesin vivo and in tissue culture

  • C. J. Louis
Papers

Synopsis

Tracer doses of isotopically labelled 5-hydroxytryptophan have been injected into rats and added to the culture medium of rat brain explants in the presence of monoamine oxidase inhibitor. Selective cellular uptake and retention of labelled precursor was demonstrated over neurones bothin vivo andin vitro. Cells which bound isotope also fluoresced in ultra-violet light after exposure to paraformaldehyde. These findings, which are discussed, indicate that the material present in labelled cells is serotonin.

Keywords

Serotonin Tissue Culture Paraformaldehyde Monoamine Cellular Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd 1970

Authors and Affiliations

  • C. J. Louis
    • 1
  1. 1.Department of PathologyUniversity of MelbourneAustralia

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