The Histochemical Journal

, Volume 2, Issue 1, pp 29–34 | Cite as

Autoradiographic localization of [3H]5-hydroxytryptophan uptake by rat neuronesin vivo and in tissue culture

  • C. J. Louis


Tracer doses of isotopically labelled 5-hydroxytryptophan have been injected into rats and added to the culture medium of rat brain explants in the presence of monoamine oxidase inhibitor. Selective cellular uptake and retention of labelled precursor was demonstrated over neurones bothin vivo andin vitro. Cells which bound isotope also fluoresced in ultra-violet light after exposure to paraformaldehyde. These findings, which are discussed, indicate that the material present in labelled cells is serotonin.


Serotonin Tissue Culture Paraformaldehyde Monoamine Cellular Uptake 
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Copyright information

© Chapman and Hall Ltd 1970

Authors and Affiliations

  • C. J. Louis
    • 1
  1. 1.Department of PathologyUniversity of MelbourneAustralia

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