Synopsis
Enzyme cytochemical studies have been carried out on eosinophils in the fowl and the duck. Peroxidase was found in all regions of the Golgi apparatus, the rough endoplasmic reticulum and the perinuclear cisternae of the early cells. In fowl eosinophil granules irregular deposits of peroxidase and arylsulphatase final reaction product were found, but the acid phosphatase deposits were even. In the duck in contrast, peroxidase was demonstrated in the external part of the granule only. Acid phosphatase and arylsulphatase were found in both the interna and the externa of the duck eosinophil granules. An ammoniacal silver nitrate reaction for the presence of the histone arginine was also studied. Silver deposits were found occupying all regions of the granules of eosinophils from both species of bird.
The presence of the hydrolytic enzymes acid phosphatase and arylsulphatase in avian eosinophil granules supports the theory that these structures are lysosomal in nature and that they correspond with mammalian eosinophils in this respect.
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References
Ackerman, G. A. &Clark, M. A. (1971). Ultrastructural localisation of peroxidase activity in normal human bone marrow cells.Z. Zellforsch. mikrosk. Anat. 117, 463–75.
Archer, G. T. &Hirsch, J. G. (1963). Isolation of granules from eosinophil leucocytes and study of their enzyme content.J. exp. Med 118, 277–93.
Archer, G. T., Air, G., Jackas, M. &Morell, D. B. (1965). Studies on rat eosinophil peroxidase.Biochim. Biophys. Acta 99, 96–101.
Atwal, O. S. &McFarland, L. Z. (1966). A morphologic and cytochemical study of erythrocytes and leucocytes ofCoturnix coturnix japonica.Am. J. vet Res. 27, 1059–65.
Bainton, D. F. &Farquhar, M. G. (1970). Segregation and packaging of granule enzymes in eosinophilic leucocytes.J. Cell Biol. 45, 54–73.
Barka, T. &Anderson, P. J. (1962). Histochemical methods for acid phosphatase using hexazonium pararosaniline as coupler.J. Histochem. Cytochem. 10, 741–53.
Black, M. M. &Ansley, H. R. (1966). Histone specificity revealed by ammoniacal silver staining.J. Histochem. Cytochem. 14, 177–81.
Bozhkov, S. &Kirev, T. (1972). Histochemical studies on bone marrow cells in different species of healthy birds.Bulg. Acad. Sci., Inst. Gen. Comp. Path. 14, 213–24.
Caro, L. G. &Palade, G. E. (1964). Protein synthesis, storage and discharge in the pancreatic exocrine cell: An autoradiographic study.J. Cell Biol. 20, 473–95.
Cotran, R. S. &Litt, M. (1969). The entry of granule-associated peroxidase into the phagocytic vacuoles of eosinophils.J. exp. Med. 129, 1291–9.
Enomoto, T. &Kitani, T. (1966). Electron microscopic studies and acid phosphatase reaction in human leucocytes. (In normal and leukaemic cells and on the phagocytosis).Acta Haem. Jap. 29, 554–70.
Ericsson, J. L. E. &Nair, M. K. (1973). Electron microscopic demonstration of acid phosphatase activity in the developing and mature heterophils of the chicken.Histochemie 37, 97–105.
Fedorko, M. E. (1968) Formation of cytoplasmic granules in human eosinophilic myelocytes. An electron microscope autoradiographic study.Blood 31, 188–94.
Geyer, G., Schaaf, P., Linss, W., Stibens, H. J., Halbhuber, K. J., Quade, R. &Christner, A. (1970). Enzyme distribution patterns in the granules of eosinophil leucocytes of the mouse.Acta histochem. 38, 189–91.
Goldfischer, S. (1965). The cytochemical demonstration of lysosomal arylsulphatase activity by light and electron microscopy.J. Histochem. Cytochem. 13, 520–2.
Graham, G. S. (1918). Benzidine as a peroxidase reagent for blood smears and tissues.J. med. Res. 39, 15–24.
Vanov, X., Bozhkov, S. &Kirev, T. (1967). Histochemical studies of the cells from the peripheral blood of certain bird species.Acad Bulg. des Sci. 12, 145–51.
Kélényi, G. &Németh, A. (1969). Comparative histochemistry and electron microscopy of the eosinophil leucocytes of vertebrates: I. A study of avian, reptile, amphibian and fish leucocytes.Acta Biol. Acad. Sci. Hung. 20, 405–22.
Klebanoff, S. J. (1967). Iodination of bacteria: a bactericidal mechanism.J. exp. Med. 126, 1063–78.
Komiyama, A. &Spicer, S. S. (1975). Microendocytosis in eosinophil leucocytes.J. Cell Biol 64, 622–35.
Macrae, E. K. (1974). Localisation of ammoniacal silver reaction product with leucocyte granules.Anat Rec. 178, 409 A.
Macrae, E. K. &Meetz, G. D. (1970). Electron microscopy of the ammoniacal silver reaction for histones in the erythropoietic cells of the chick.J. Cell Biol. 45, 235–45.
Maxwell, M. H. &Trejo, F. (1970). The Ultrastructure of white blood cells and thrombocytes of the domestic fowl.Brit. vet. J. 126, 583–92.
Maxwell, M. H. (1978). The development of eosinophils in the bone marrow of the fowl and the duck.J. Anat. (London) 125, (in press).
Merkal, R. S. &Mora, F. C. (1962). Cytochemistry of erythrocytes and leucocytes of the white leghorn chicken.Exp. Mol. Path. 1, 497–508.
Miller, F. &Herzog, V. (1969). Die Lokalisation von Peroxidase und saurer Phosphatase in eosinophilen Leukocyten Während der Reifung.Z. Zellforsch. mikrosk. Anat. 97, 84–110.
Miller, F. & Herzog, V. (1970). Localisation of acid phosphatase and peroxidase in eosinophil and neutrophil leucocytes of the cat.7th Congress Int. de microscopie électronique, Grenoble, pp. 573–4.
Parmley, R. T. &Spicer, S. S. (1974). Cytochemical and ultrastructural identification of a small type granule in human late eosinophils.Lab. Invest. 30, 557–67.
Pitt, D. (1975).Lysosomes and Cell Function. London: Longman.
Rabinovitch, M. &Andreucci, D. (1949). A histochemical study of ‘acid’ and ‘alkaline’ phosphatase distribution in normal human bone marrow smears.Blood 4, 580–94.
Rogovine, V. V., Muravieff, R. A., Frolova, W. M., Geranine, N. G. &Piruzyan, L. A. (1975). Ultrastructural cytochemistry of peroxidase and acid phosphatase in mice maturing eosinophils.Experientia 31, 1031–2.
Seeman, P. M. &Palade, G. E. (1967). Acid phosphatase localisation in rabbit eosinophils.J. Cell Biol. 34, 745–56.
Undritz, E. (1952).Sandoz Atlas of Haematology. Basle, Switzerland: Frobenius Ltd.
Vercauteren, R. (1950). A cytochemical approach to the problem of the significance of blood and tissue eosinophilia.Enzymologia 14, 340–6.
Vercauteren, R. (1953). The properties of the isolated granules from blood eosinophils.Enzymologia 16, 1–13.
Yamada, E. &Yamauchi, R. (1966). Some observations on the cytochemistry and morphogenesis of the granulocytes in the rat bone marrow as revealed by electron microscopy.Jap. J. Haemat 29, 530–41.
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Maxwell, M.H. Electron cytochemistry of developing and mature eosinophils in the bone marrow of the fowl and the duck. Histochem J 10, 63–77 (1978). https://doi.org/10.1007/BF01003415
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DOI: https://doi.org/10.1007/BF01003415