The Histochemical Journal

, Volume 16, Issue 6, pp 587–599 | Cite as

The histochemistry of complex carbohydrates in certain organs of homozygous brachymorphic (bm/bm) mice

  • K. Yamada
  • S. Shimizu
  • K. S. Brown
  • K. Kimata
Papers

Summary

Homozygous brachymorphic (bm/bm) mice are characterized by disproportionately short stature and by undersulphated proteochondroitin sulphate in cartilage with a defect in the synthesis of the sulphate donor (3′-phosphoadenosine-5′-phosphosulphate: PAPS). In adult mice of this mutant strain, several organs have been examined by light microscopical methods of complex carbohydrate histochemistry, in comparison with those from control (C57BL/6J) mice of comparable ages. The organs included the trachea, aorta, skin, stomach colon and cornea. In the nutant mice, connective tissue elements and components of epithelial tissues exhibited apparently weaker positive reactions for ester sulphate groupings of complex carbohydrates, as compared with those in the control mice. From these results, it is concluded that the undersulphation in complex carbohydrates is widespread throughout the connective and epithelial tissues of the mutant mice.

Keywords

Mutant Strain Mutant Mouse Adult Mouse Positive Reaction Short Stature 

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Copyright information

© Chapman and Hall Ltd 1984

Authors and Affiliations

  • K. Yamada
    • 1
  • S. Shimizu
    • 2
  • K. S. Brown
    • 3
  • K. Kimata
    • 4
  1. 1.Department of AnatomyNagoya City University Medical SchoolNagoyaJapan
  2. 2.Laboratory of Ultrastructure ResearchAichi Cancer Center Research InstituteNagoyaJapan
  3. 3.Laboratory of Developmental Biology and Abnormalities, National Institute of Dental ResearchNational Institute of HealthBethesdaU.S.A.
  4. 4.Department of Chemistry, Faculty of ScienceNagoya UniversityNagoyaJapan

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