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Capping of saccharides on the plasma membrane of lymhocytes as studied by fluorescein-labelled lectins

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The capping of saccharides on the plasma membrane of rat splenic lymphocytes was studied by means of fluorescein-labelled lectins. Treatment of unfixed splenic lymphocytes with any one of the three lectins, concanavalin A (Con A), Ricinus communis agglutinin (RCA) and wheat germ agglutinin (WGA) led to the formation of caps of each sacchande receptor on the plasma membrane. Treatment of unfixed lymphocytes with Con A was found to result in the formation of caps of saccharide receptors for RCA, whereas cap formations were never noted in such double treatment of the cells with all other combined uses of two lectins. These results are taken to indicate that the saccharide receptors for Con A are associated with those for RCA in the plasma membrane of rat splenic lymphocytes.

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References

  • Bernhard, W. &Avrameas, S. (1971). Ultrastructural visualization of cellular carbohydrate components by means of concanavalin A.Expl Cell Res. 64, 232–6.

    Google Scholar 

  • De Petris, S. (1975). Concanavalin A receptors, immunoglobulins and ϑ antigen of the lymphocyte surface. Interaction with concanavalin A and with cytoplasmic structure.J. Cell Biol. 65, 123–46.

    Google Scholar 

  • Frye, L. D. &Edidin, M. (1970). The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons.J. Cell Sci. 7, 319–35.

    Google Scholar 

  • Karnovsky, M. J. &Unanue, E. R. (1973). Mapping and migration of lymphocyte surface macromolecules.Fedn Proc. Fedn Am. Socs exp. Biol. 32, 55–9.

    Google Scholar 

  • Kiernan, J. A. (1975). Localization of α-D-glucosyl and α-D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase.Histochemistry 44, 39–45.

    Google Scholar 

  • Le Vine, D., Kaplan, M. J. &Greenaway, P. J. (1972). The purification and characterization of wheat germ agglutinin.Biochem. J. 129, 847–56.

    Google Scholar 

  • Loor, F. (1973). Lectin-induced lymphocyte agglutination.Expl Cell Res. 82, 415–25.

    Google Scholar 

  • Loor, F. (1974). Binding and redistribution of lectins on lymphocyte membrane.Eur. J. Immunol. 4, 210–20.

    Google Scholar 

  • Nicolson, G. L. &Blaustein, J. (1972). The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces.Biochim. Biophys. Acta 266, 543–7.

    Google Scholar 

  • Parmley, R. R., Martin, B. J. &Spicer, S. S. (1974). Staining of blood cell surfaces with a lectin-horseradish peroxidase method.J. Histochem. Cytochem. 21, 912–22.

    Google Scholar 

  • Sharon, N. &Lis, H. (1972). Lectins: cell-agglutinating and sugar specific proteins.Science 177, 949–59.

    Google Scholar 

  • Shimizu, S. &Yamada, K. (1976). Distribution and nature of saccharides of plasma membranes of rat ascites hepatoma cells as detected by ferritin-conjugated lectins and dialyzed iron.Expl Cell Res. 97, 322–8.

    Google Scholar 

  • Shinitzky, M., Inbar, M. &Sachs, L. (1973). Rotational diffusion of lectins bound to the surface membrane of normal lymphocytes.FEBS Letters 34, 247–50.

    Google Scholar 

  • Taylor, R. B., Duffus, W. D. H., Raff, M. C. &De Petris, S. (1971). Redistribution and pinocytosis of lymphocyte surface immunoglobulin molecules induced by anti-immunoglobulin antibody.Nature New Biol. 233, 225–9.

    Google Scholar 

  • Unanue, E. R., Perkins, W. D. &Karnovsky, M. J. (1972). Ligand-induced movement of lymphocyte membrane macromolecules. I. Analysis by immunofluorescence and ultrastructural radioautography.J. exp. Med. 136, 885–906.

    Google Scholar 

  • Yahara, I. &Edelman, G. M. (1973). The effects of concanavalin A on the mobility of lymphocyte surface receptors.Expl Cell Res. 81, 143–55.

    Google Scholar 

  • Yahara, I. &Edelman, G. M. (1975). Modulation of lymphocyte receptor mobility by locally bound concanavalin A.Proc. natn. Acad. Sci. U.S.A. 72, 1579–83.

    Google Scholar 

  • Yamada, K. &Shimizu, S. (1976). Concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB)-alcian blue (AB). A reliable method for dual staining of complex carbohydrates.Histochemistry 47, 159–69.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, Nagova, Japan

    N. Morimoto & S. Shimizu

  2. Laboratory of Histology, Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

    K. Yamada

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  1. N. Morimoto
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  2. S. Shimizu
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  3. K. Yamada
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Morimoto, N., Shimizu, S. & Yamada, K. Capping of saccharides on the plasma membrane of lymhocytes as studied by fluorescein-labelled lectins. Histochem J 10, 223–228 (1978). https://doi.org/10.1007/BF01003307

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  • DOI: https://doi.org/10.1007/BF01003307

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