Skip to main content
SpringerLink
Log in

The measurement of histochemically-stained NADP-dependent isocitrate dehydrogenase in the sebaceous glands of hairless hamster

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

The effect of substrate, co-factor and Nitroblue Tetrazolium concentration on the production of formazan by the action of NADP-dependent isocitrate dehydrogenase was studied in the sebaceous glands of the hairless hamster. The measurement (in average optical density units per unit area) of formazan in histochemically stained skin sections was carried out by television scanning microdensitometry (Quantimet 720D). Having established optimal conditions for this enzyme, a second study was carried out to determine the effect of different power objectives and wide-band wavelength light instead of white light on the average optical density per unit area, recorded by the instrument, of the formazan produced in a defined number of sebaceous glands in a single skin section.

It was found that there was no difference in the average optical density per unit area recorded by the instrument at different power objectives and a peak value of average optical density per unit area could be obtained using a K4 or K5 Balzer filter (550–650 nm).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altman, F. P. (1976). The quantification of formazan in tissue sections by microdensitometry. III. The effect of objective power and scanning spot size.Histochem. J. 8, 507–11.

    Google Scholar 

  • Bond, C. P. (1974). Television microdensitometry measurements in biological image analysis.Fortschr. Quantitative Bildanalyse-Vorträge des Imanco Symposium-Frankfurt/M. 1973. pp. 33–41.

  • Clark, C. &Picton, W. (1977). Comparison of two techniques for the quantitative measurement of acid phosphatase in normal and regenerating epithelium of the female hairless hamster.Histochem. J. 9, 301–8.

    Google Scholar 

  • Fisher, C. &Bond, C. P. (1972). The Quantimet 720D for densitometry in the life sciences.Microscope 20, 203–16.

    Google Scholar 

  • Picton, W., Devitt, H. &Forgie, M. A. (1976). Practical applications of the Quantimet 720, an image analysing computer in the field of investigative dermatology.Br. J. Derm. 95, 341–8.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Pharmacology, Scientific Development Group, Organon Laboratories Limited, Newhouse, Lanarkshire, Scotland

    W. Picton & C. Clark

Authors
  1. W. Picton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Picton, W., Clark, C. The measurement of histochemically-stained NADP-dependent isocitrate dehydrogenase in the sebaceous glands of hairless hamster. Histochem J 10, 191–199 (1978). https://doi.org/10.1007/BF01003304

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01003304

Keywords

Search

Navigation