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Iron exclusion by proliferative foci and neoplastic lesions induced by 7,12-dimethylbenz (a) anthracene in the rat adrenal cortex

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Summary

Proliferative and neoplastic adrenal cortical lesions were produced in female Sprague-Dawley rats by 7,12-dimethylbenz(a)anthracene. These lesions were studied for their ability to store histochemically demonstrable cellular iron compared with normal adrenocortical cells following iron loading by subcutaneous injection of iron-dextran. Stainable iron was excluded, under conditions in which normal cortical cells were heavily loaded with iron, in three histological types of adrenalcortical lesions; eosinophilic and basophilic proliferative foci; adenomas and lesions transitional between foci and tumours. The property of iron exclusion may be useful in studying neoplastic development in the adrenal gland.

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References

  • Chan, P. C. &Cohen, L. A. (1974) Effect of dietary, fat, antiestrogen, and antiprolactin on the development of mammary tumors in rats.J. natn Cancer Inst. 52, 25–30.

    Google Scholar 

  • Dao, T. L. (1969) Mammary cancer induction by 7,12-dimethylbenz(a)anthracene: Relation to age.Science 165, 810–11.

    Google Scholar 

  • Dunn, T. B. (1970) Normal and pathologic anatomy of the adrenal gland of the mouse, including neoplasms.J. natn Cancer Inst. 44, 1323–89.

    Google Scholar 

  • Frantz, M. J. &Kirschbaum, A. (1949) Sex hormone secretion by tumours of the adrenal cortex of mice.Cancer Res. 9, 251–66.

    Google Scholar 

  • Gomori, G. (1936) Microtechnical demonstration of iron.Am. J. Path. 12, 655–63.

    Google Scholar 

  • Hirota, N. &Williams, G. M. (1979) The sensitivity and heterogeneity of histochemical markers for altered foci involved in liver carcinogenesis.Am. J. Path. 95, 317–28.

    Google Scholar 

  • Hollander, C. F. &Snell, K. C. (1976) Tumours of the adrenal gland. InTumours of the Rat (edited byTurusov, V. S.), Vol. 1, Part 2, pp. 273–80. Lyon: IARC Scientific Publications.

    Google Scholar 

  • Houssay, B. A., Houssay, A. B., Cardeza, F. A. &Pinto, R. M. (1955) Tumeurs surrénales éstroǵeniques et tumeurs hypophysaires chez les animaux castrés.Schweiz. med. Wschr. 85, 291–6.

    Google Scholar 

  • Iglesias, R. &Mardones, E. (1958) Spontaneous and transplantable functional tumour of the adrenal cortex in the A X C rat.Br. J. Cancer 12, 20–7.

    Google Scholar 

  • Lipsky, M. M., Hinton, D. E., Goldblatt, P. J., Klaunig, J. E. &Trump, B. H. (1979) Iron negative foci and nodules in safrole-exposed mouse liver made siderotic by iron-dextran injection.Path. Res. Pract. 164, 178–85.

    Google Scholar 

  • Malvaldi, G. (1981) Appearance of iron-free hyperplastic hepatocytes following ferric nitrilotracetate and 2-acetaminofluorene sequential administration.Eur. J. Cancer 17, 481–3.

    Google Scholar 

  • Matsuyama, M. &Suzuki, H. (1970) Adrenal tumours and endrocrine lesions induced in Syrian hamsters by urethane injected during suckling period.Br. J. Cancer 24, 312–8.

    Google Scholar 

  • Mody, J. K. (1967) A new type of transplantable adrenal tumor and its comparative histopathology.Cancer Res. 29, 1254–61.

    Google Scholar 

  • Moon, H. D., Simpson, M. E., Li, C. H. &Evans, H. (1950) Neoplasms in rats treated with pituitary growth hormone. II. Adrenal glands.Cancer Res. 10, 364–70.

    Google Scholar 

  • Mori, H., Tanaka, T., Takahashi, M. &Williams, G. M. (1983) Exclusion of cellular iron and reduced gamma-glutamyl transpeptidase activity in rat pancreas acinar cell hyperplastic nodules and adenomas induced by azaserine.Gann 74, 487–501.

    Google Scholar 

  • Nichals, T. M. (1971) The morphological effects of estrogen removal on an estrogen-dependent adrenocortical carcinoma in rats.Cancer Res. 31 1042–50.

    Google Scholar 

  • Nigam, S. K., Babu, K. A., Bhatt, D. K., Karnik, A. B., Thakore, K. N., Lakkad, B. C., Kashyap, S. K. &Chatterjee, S. K. (1981) Pattern of glycogen, and iron accumulation in early appearing BHC induced liver lesions and liver tumours.Indian J. Med. Res. 74, 289–96.

    Google Scholar 

  • Noble, R. L. (1967) Induced transplantable estrogen-dependent carcinoma of the adrenal cortex in rats.Proc. Am. Ass. Cancer Res. 8, 51.

    Google Scholar 

  • Ohmori, T., Rice, J. M. &Williams, G. M. (1981) Histochemical characteristics of spontaneous and chemically induced hepatocellular neoplasms in mice and the development of neoplasms with γ-glutamyl transpeptidase activity during phenobarbital exposure.Histochem. J. 13, 85–99.

    Google Scholar 

  • Snell, K. C. &Stewart, H. L. (1959) Variations in histologic pattern and functional effects of a transplantable adrenal cortical carcinoma in intact hypophysectomized, and newborn rats.J. natn Cancer Inst. 22 1119–32.

    Google Scholar 

  • Takasugi, N., Takeuchi, H. &Taguchi, O. (1975) Effect of steroid hormone on growth on adrenocortical carcinoma transplants in mice.Gann 66, 57–67.

    Google Scholar 

  • Takizawa, S. (1978) Endocrine organs. InChemical Carcinogenesis and Cancer Genesis (edited byOdashima, S. andHashimoto, Y.), pp. 363–75. Tokyo: Gakkai Publ. Center (in Japanese).

    Google Scholar 

  • Timme, A. H. (1978) Hyperplastic foci in precancerous rat liver: light microscopic and electron microscopic study.J. natn. Cancer. Inst. 61, 407–9.

    Google Scholar 

  • Vijayaraghavan, S. &Rivera, E. M. (1981) Preferential accumulation of iron in hyperplastic tissue of rat mammary gland (41250).Proc. Soc. exp. Biol. Med. 168, 151–4.

    Google Scholar 

  • Williams, G. M. (1976) Functional markers and growth behavior of preneoplastic hepatocytes.Cancer Res. 36, 2540–3.

    Google Scholar 

  • Williams, G. M. (1980) The pathogenesis of rat liver cancer caused by chemical carcinogens.Biochim. biophys. Acta 605, 167–89.

    Google Scholar 

  • Williams, G. M., Hirota, N., &Rice, J. M. (1979) The resistance of spontaneous mouse hepatocellular neoplasms to iron-accumulation during rapid iron-loading by parenteral administration and their transplantability.Am. J. Path. 94, 65–74.

    Google Scholar 

  • Williams, G. M. &Yamamoto, R. S. (1972) Absence of stainable iron from preneoplastic and neoplastic lesions in rat liver with 8-hydroxyquinoline-induced siderosis.J. natn. Cancer Inst. 49, 685–92.

    Google Scholar 

  • Wooley, G. M. (1950) Experimental endocrine tumors with special reference to the adrenal cortex.Rec. Prog. Horm. Res. 5, 383–405.

    Google Scholar 

  • Yamamoto, R. S., Williams, G. M., Frankel, H. H. &Weisburger, J. H. (1971) 8-hydroxyquinoline: Chronic toxicity and inhibitory effect on the carcinogenicity ofN-2-fluorenylacetamide.Toxicol. Appl. Pharmac. 19, 687–98.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Ehime Prefectural Central Hospital, Kasuga-Cho 83, 790, Matsuyama, Ehime, Japan

    Keizo Furuya

  2. Division of Experimental Pathology and Toxicology, American Health Foundation, Naylor Dana Institute for Disease Prevention, Dana Road, 10595, Valhalla, New York, USA

    Gary M. Williams

Authors
  1. Keizo Furuya
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  2. Gary M. Williams
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Furuya, K., Williams, G.M. Iron exclusion by proliferative foci and neoplastic lesions induced by 7,12-dimethylbenz (a) anthracene in the rat adrenal cortex. Histochem J 17, 213–221 (1985). https://doi.org/10.1007/BF01003220

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  • DOI: https://doi.org/10.1007/BF01003220

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