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Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chainO-acylated sialic acids

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Summary

Statistically significant correlations were obtained between a chemical assay for the proportion of colonic epithelial glycoprotein sialic acids with side chainO-acyl substituents and two histochemical methods, the PBT-KOH-PAS sequence (r s=0.7485 forN=31,P=0.01, one-sided test) and the PAPT-KOH-Bh-PAS procedure (r s=0.7024 forN=34). A positive correlation (r s=0.8654 forN=30,P=0.01) was also obtained between the results of the two histochemical procedures. It is concluded that, on average, histochemical observations are a reliable semiquantitative comparative method for the estimation of side chainO-acetylated sialic acids.

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References

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, University of British Columbia, V6T 1W5, Vancouver, BC, Canada

    P. E. Reid, C. W. Ramey, D. A. Owen, W. L. Dunn & D. A. Lazosky

  2. Department of Surgery, Faculty of Medicine, University of British Columbia, V6T 1W5, Vancouver, BC, Canada

    M. G. Clay

Authors
  1. P. E. Reid
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  2. C. W. Ramey
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  3. D. A. Owen
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  4. W. L. Dunn
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  5. D. A. Lazosky
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  6. M. G. Clay
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Reid, P.E., Ramey, C.W., Owen, D.A. et al. Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chainO-acylated sialic acids. Histochem J 17, 183–187 (1985). https://doi.org/10.1007/BF01003217

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  • DOI: https://doi.org/10.1007/BF01003217

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