Summary
Tissues embedded in resin are convenient for routine use when the presence or absence of fibrin in them is to be confirmed using the electron microscope. To visualize fibrin using the light microscope, sections (1.0–2.0 μm) from such specimens should be stained with Methylene Blue-Azure II-Basic Fuchsin (MBBF). Staining with MBBF is more controllable than with other methods and it requires only two short staining steps. Compared with Giemsa, MBBF provides a polychromatic, as opposed to a monochromatic end-result, sharply contrasting fibrin (blue) against collagen (pink-violet).
Similar content being viewed by others
References
Bach, C. S. & Jasani, M. K. (1976) Role of lymphocytes in accumulation of fibrin in rabbit skin homografts.Br. J. Pharmac. 58, 292P.
Bullock, G. R., Jasani, M. K. &Lewis, G. P. (1977) Morphology of immune lymphocytes in homograft lymph.Cell Biol. Int. Rep. 1, 123–35.
Colvin, R. B., Johnson, R. A., Mihm, M. C. Jr &Dvorak, H. F. (1973) Role of the clotting system in cell-mediated hypersensitivity. I. Fibrin deposition in delayed skin reactions in man.J. exp. Med. 138, 686–98.
Dvorak, H. F., Dvorak, A. M., Simpson, B. A., Richerson, H. B., Leskovitz, S. &Karnovsky, M. J. (1970) Cutaneous basophil hypersensitivity. II. A light and electron microscopic description.J. exp. Med. 132, 558–82.
Dvorak, H. F. &Mihm, M. C. Jr (1972) Basophilic leukocytes in allergic contact dermatitis.J. exp. Med. 135, 235–54.
Humphrey, C. D. &Pittman, F. E. (1974) A simple methylene blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections.Stain Technol. 49, 9–14.
Jasani, M. K. & Bach, C. S. (1979) Prostaglandins, rheumatoid arthritis, fibrin and lymphoedema. InArachidonic Acid Metabolism in Inflammation and Thrombosis (edited byBrune, K. andBaggiolini, M.),Agents and Actions Symposia 4, 222–31.
Jasani, M. K. &Lewis, G. P. (1971) Lymph flow and changes in intracellular enzymes during rejection of rabbit skin homografts.J. Physiol. 219, 525–54.
Jasani, M. K., Roberts, J. M., Talbot, M. D. &Tweed, M. F. (1978) Recognition of anti-rheumatic drugs using the homograft reaction. InRecognition of Anti-Rheumatic Drugs (edited byDumonde, D. C. andJasani, M. K.), pp. 193–228. Lancaster, MTP Press Ltd.
Lendrum, A. C., Fraser, D. S., Slidders, W. &Henderson, R. (1962) Studies on the character and staining of fibrin.J. clin. Path. 15, 401–13.
Mcfarlane, D. (1944) An easily controlled regressive trichromic staining method.Stain Technol. 19, 29–37.
Mollenhauer, H. H. (1964) Plastic embedding mixtures for use in electron microscopy.Stain Technol. 39, 111–14.
Porter, K. R. &Hawn, C. Van X. (1949) Sequence in the formation of clots from purified bovine fibrinogen and thrombin: a study with the electron microscope.J. exp. Med. 90, 225–38.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bullock, G.R., Jasani, M.K., Roberts, J.M. et al. An improved method for the demonstration of fibrin in biopsies of rabbit skin homografts. Histochem J 17, 143–151 (1985). https://doi.org/10.1007/BF01003214
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01003214