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The histochemistry of asparagine-linked oligosaccharides of glycoproteins in mammalian and avian tissues as studied by digestion with almond glycopeptidase

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Summary

In a series of mammalian and avian tissues, asparagine-linked oligosaccharides of glycoproteins have been localized histochemically by mearis of combined periodic acid-Schiff (PAS) and almond glycopeptidase digestion procedures. According to the present results, the particular saccharide residues were found to be localized primarily in glycoproteins of connective and muscular tissue elements but were hardly visualizable or absent in glycoproteins of epithelial tissue elements. In view of the substrate specificity of almond glycopeptidase and the known staining selectivity of the PAS reaction, the majority of connective and muscular tissue elements are thought to be the main sites where plasma types of glycoproteins are involved.

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References

  • Casselman, W. G. B. (1959) Carbohydrates. InHistochemical Technique, pp. 91–109. London: Methuen.

    Google Scholar 

  • Clamp, J. R. (1977) Mucus in health and diseases.Adv. exp. Med. Biol. 89, 1–15.

    Google Scholar 

  • Leppi, T. J. (1968) Morphochemical analysis of mucous cells in the skin and slime glands of hag-fishes.Histochemie 15, 68–78.

    Google Scholar 

  • Lillie, R. D. &Fullmer, H. M. (1976) Fixation. InHistopathologic Technic and Practical Histochemistry 4th edn. pp. 25–68. New York: McGraw-Hill.

    Google Scholar 

  • McManus, J. F. A. (1948) Histological and histochemical uses of periodic acid.Stain Technol. 23, 99–108.

    Google Scholar 

  • Pearse, A. G. E. (1968) Carbohydrates and mucosubstances. InHistochemistry, Theoretical and Applied. Vol. 1 3rd edn, pp. 294–380. Edinburgh, London, New York: Churchill Livingstone.

    Google Scholar 

  • Pearse, A. G. E. (1980) Enzymes as histochemical reagents. InHistochemistry, Theoretical and Applied. Vol. 1.Preparative and Optical Technology. 4th edn, pp. 270–302. Edinburgh, London, New York: Churchill Livingstone.

    Google Scholar 

  • Spicer, S. S. &Warren, W. (1960) The histochemistry of sialic acid containing mucoproteins.J. Histochem. Cytochem. 8, 135–7.

    Google Scholar 

  • Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967) Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (edited byWagner, B. M. andSmith, D. E.), pp. 251–303. Baltimore: Wüliams & Wilkins.

    Google Scholar 

  • Takahashi, N. (1977) Demonstration of a new amidase acting on glycopeptides.Biochem. biophys. Res. Commun. 76, 1194–201.

    Google Scholar 

  • Takahashi, N. &Nishibe, H. (1981) Almond glycopeptidase acting on aspartylglucosylamine linkages. Multiplicity and substrate specificity.Biochem. biophys. Acta 657, 456–67.

    Google Scholar 

  • Takahashi, N., Shimizu, S. &Yamada, K. (1982) Asparagine-linked oligosaccharides in human placenta and umbilical cord as demonstrated by almond glycopeptidase digestion.FEBS Letts 146, 139–42.

    Google Scholar 

  • Uchida, Y., Tsukada, Y. &Sugimori, T. (1977) Distribution of neuraminidase inArthrobacter and its purification by affinity chromatography.J. Biochem. 82, 1425–33.

    Google Scholar 

  • Uchida, Y., Tsukada, Y. &Sugimori, T. (1979) Enzymatic properties of neuraminidases fromArthrobacter ureafaciens.J. Biochem. 86, 1573–85.

    Google Scholar 

  • Yamada, K. (1972) Histochemical and cytochemical methods for the studies of mucosaccharides.Prot. Nucl. Acid. Enzyme 17, 775–90.

    Google Scholar 

  • Yamada, K. (1981) Histochemistry of glycoproteins.The Cell 13, 358–65.

    Google Scholar 

  • Yamada, K., Shimizu, S. &Takahashi, N. (1982) Demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord with almond glycopeptidase digestion: a histochemical and biochemical study.J. Histochem. Cytochem. 30, 591.

    Google Scholar 

  • Yamada, K., Shimizu, S. &Takahashi, N. (1983) Histochemical demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues by means of almond glycopeptidase digestion.Histochem. J. 15, 1239–50.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan

    H. Murata

  2. Department of Biochemistry, Nagoya City University Medical School, Nagoya, Japan

    N. Takahashi

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  1. H. Murata
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  2. N. Takahashi
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  3. K. Yamada
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Murata, H., Takahashi, N. & Yamada, K. The histochemistry of asparagine-linked oligosaccharides of glycoproteins in mammalian and avian tissues as studied by digestion with almond glycopeptidase. Histochem J 17, 121–130 (1985). https://doi.org/10.1007/BF01003212

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  • DOI: https://doi.org/10.1007/BF01003212

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