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The Histochemical Journal

, Volume 17, Issue 4, pp 389–442 | Cite as

Cell and tissue fixation, 1972–1982

  • D. Hopwood
Review

Conclusions

There have been changes in the practice of fixation over the past 10 years. There seem to be at least two different pressures working. On the one hand, there is increasing diversity in cell biological techniques which in turn demands more variable fixation procedures. Some of these have been outlined. Some of this change in practice has percolated through to pathology where it has been found to be diagnostically useful. In surgical pathology on the other hand, there is the continuing financial pressure for more rapid through-put of specimens which includes more rapid fixation, often with the loss of potential for subsequent chemical investigations. These are the horns of the dilemma; both are wanted at the same time.

It seems that there is no magical fixative in sight which will permit all investigations on all tissues. Rather, it seems that the future will hold increasing diversity in fixation procedures which will demand that practitioners be well informed as to possibilities which, hopefully, may lend to better understanding of the problems and mechanisms of fixation.

Keywords

Percolate Fixation Procedure Biological Technique Chemical Investigation Tissue Fixation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abramczuk, J. (1972) Effect of formalin fixation on the dry mass of isolated rat liver nuclei.Histochemie 29, 207–12.Google Scholar
  2. Anderson, W. A., Trantalis, J. &Kang, Y. H. (1975) Ultrastructural localization of endogenous mammary gland peroxidase during lactogenesis in the rat.J. Histochem. Cytochem. 23, 295–302.Google Scholar
  3. Angermüller, S. &Fahimi, H. D. (1982). Imidazole buffered osmium tetroxide; an excellent stain for the visualization of lipids in transmission electron microscopy.Histochem. J. 14, 825–36.Google Scholar
  4. Anon (1980) News in Brief. Academy recommends cut in formaldehyde exposure.Nature 284, 587.Google Scholar
  5. Arancia, G., Valente, F. R. &Crateri, P. T. (1979) Effects of glutaralehyde and glycerol on freeze fracturedE. coli.J. Microsc. 118, 161–76.Google Scholar
  6. Artvinli, S. (1975) Biochemical aspects of aldehyde fixation and a new formaldehyde fixative.Histochem. J. 7, 435–50.Google Scholar
  7. Baker, J. R. (1960)Principles of Biological Microtechnique. London: Methuen.Google Scholar
  8. Baker, J. R. J. &Pay, G. F. (1980) The effects of glutaraldehyde and formaldehyde fixation on the retention and subcellular location of [3H]-5HT in rabbit platelets.J. Cell Sci. 41, 263–72.Google Scholar
  9. Bauer, P. S. &Stacey, T. R. (1977) The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy.J. Microsc. 109, 315–27.Google Scholar
  10. Beertsen, W. &Tonino, G. J. M. (1975) Effects of fixation and demineralization on the intensity of ARG labelling over the periodontal ligament of the mouse incisor after administration of [3H]-proline.Arch. Oral Biol. 20, 189–93.Google Scholar
  11. Behnke, O. &Zelander, T. (1970) Presevation of intercellular substances by the cationic dye Alcian blue in preparative procedures for electron microscopy.J. Ultrastruct. Res. 31, 424–38.Google Scholar
  12. Bender, J., Reinhart, C. F. &Mullin, L. S. (1982) Formaldehyde toxicity.J. Am. med. Ass. 248, 308–9.Google Scholar
  13. Bernard, G. R. (1974) Microwave irradiation as a generator of heat for histological fixation.Stain Technol. 49, 215–24.Google Scholar
  14. Berns, K. I. &Thomas, C. A. (1961) A study of single polynucleotide chains derived from T2 and T4 bacteriophage.J. molec. Biol. 3, 289–300.Google Scholar
  15. Berod, A., Hartman, B. K. &Pujol, J. F. (1981) Importance of fixation in immunohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase.J. Histochem. Cytochem. 29, 844–50.Google Scholar
  16. Berteloot, A. &Hugon, J. S. (1975) Effect of glutaraldehyde and lead on the activity of hepatic glucose-6-phosphatase.Histochemistry 43, 197–214.Google Scholar
  17. Blanquet, P. R. (1976) Ultrahistochemical study on the Ruthenium red surface staining. II. Nature and affinity of the electron dense marker.Histochemistry 47, 175–89.Google Scholar
  18. Blest, A. D. &Davie, P. S. (1977) A new fixative solution to precede the reduced silver impregnation of arthropod central nervous system.Stain Technol. 52, 273–5.Google Scholar
  19. Bohman, S. O. (1974) The ultrastructure of the rat renal medulla as observed after improved fixation methods.J. Ultrastruct. Res. 47, 329–60.Google Scholar
  20. Bohn, W. (1978) A fixation method for improved antibody penetration in electron microscopic immunoperoxidase studies.J. Histochem. Cytochem. 26, 293–7.Google Scholar
  21. Bonilla, E. (1977) Staining of transverse tubular system of skeletal muscle by tannic acid-glutaraldehyde fixation.J. Ultrastruct. Res. 58, 162–5.Google Scholar
  22. Bowes, J. A. &Cater, C. W. (1965) Crosslinking of collagen.J. appl. Chem. 15, 296–304.Google Scholar
  23. Boyde, A. &Maconnachie, E. (1980) Treatment with lithium salts reduces ethanol dehydration shrinkage of glutaraldehyde fixed tissue.Histochemistry 66, 181–7.Google Scholar
  24. Brandtzaeg, P. (1982) Tissue preparation methods for immunohistochemistry. InTechniques in Immunocytochemistry (edited byBullock, G. R. &Petrusz, P.), pp. 2–75. London: Academic Press.Google Scholar
  25. Branner-Jorgensen, S. (1978) On the mechanism of protein corss-linking with glutaraldehyde.Enzyme Eng. 4, 393–4.Google Scholar
  26. Bretherick, L. (1980)Hazards in the chemical laboratory. 3rd edition. London: Chemical Society.Google Scholar
  27. Bretscher, M. S. &Whytock, S. (1977) Membrane associated vesicles in fibroblasts.J. Ultrastruct. Res. 61, 215–7.Google Scholar
  28. Brown, L. M. &Hill, L. (1981) Mercuric chloride in alcohol and chloroform used as a rapidly acting fixative for contracting muscle fibres.J. Microsc. 125, 319–36.Google Scholar
  29. Bulletin of Royal College of Pathologists (1981)34, 3–7.Google Scholar
  30. Burton, P. R., Hickley, R. E. &Pierson, G. B. (1975) Tannic acid-stained microtubules with 12, 13 and 15 photofilaments.J. Cell Biol. 65, 227–33.Google Scholar
  31. Cameron, H. M. &McGoogan, E. (1981) A prospective study of 1152 hospital autopsies. II. Analysis of inaccuracies in clinical diagnosis and their significance.J. Path. 133, 285–300.Google Scholar
  32. Chang, C. H., Beer, M. &Marzilli, L. G. (1977) Osmium labeled polynucleotides. The reaction of osmium tetroxide with DNA and synthetic polynucleotides in the presence of tertiary nitrogen donor ligands.Biochemistry 16, 33–8.Google Scholar
  33. Chaw, Y. F. M., Crane, L. C., Lange, P. &Shapiro, R. (1980) Isolation and identification of crosslinks from formaldehyde treated nucleic acids.Biochemistry 19, 5525–31.Google Scholar
  34. Christie, K. N. &Stoward, P. J. (1974) A quantitative study of the fixation of acid phosphatases by formaldehyde and its relevance to histochemistry.Proc. R. Soc. Ser., B 186, 137–64.Google Scholar
  35. Clark, A. W. (1976) Changes in the structure of neuromuscular junctions caused by variations in osmotic pressure.J. Cell Biol. 69, 521–38.Google Scholar
  36. Collins, J. S. &Goldsmith, T. H. (1981) Special properties of fluorescence induced by glutaraldehyde fixation.J. Histochem. Cytochem. 29, 411–4.Google Scholar
  37. Collins, V. P., Arborgh, D. &Brunk, U. (1977) A comparison of the effects of three widely used glutaraldehyde fixatives on cellular volume and structure.Acta path. microbiol. scand. Ser. A 85, 157–68.Google Scholar
  38. Conger, K. A., Garcia, J. U., Lossinsky, A. S. &Kaufman, F. C. (1978) The effect of aldehyde fixation on selected substrates for energy metabolism and aminoacids in mouse brain.J. Histochem. Cytochem. 26, 423–33.Google Scholar
  39. Cope, G. H. &Williams, M. A. (1968) Quantitative studies on neutral lipid preservation in electron microscopy.J. R. microsc Soc. 85, 259–77.Google Scholar
  40. Coulter, H. D. &Elde, R. P. (1979) Ultrastructural localization of neurophysin-like immunoreactivity in the rat posterior pituitary.J. Histochem. Cytochem. 27, 1293–5.Google Scholar
  41. Coupland, R. E. (1965)The Natural History of the Chromaffin Cell. pp. 212–64. London: Longmans.Google Scholar
  42. Coupland, R. E. &Hopwood, D. (1966) The mechanism of the differential staining reaction for adrenalin and noradrenalin storing granules in tissues fixed in glutaraldehyde.J. Anat. 100, 227–43.Google Scholar
  43. Coupland, R. E., Kobayashi, S. &Crowe, J. (1976) On the fixation of catecholamines including adrenalin in tissue sections.J. Anat. 122, 403–13.Google Scholar
  44. Dae, M. W., Heymann, M. A. &Jones, A. L. (1981) A new technique for perfusion fixation and contrast enhancement of foetal lamb myocardium for electron microscopy.J. Microsc. 127, 301–5.Google Scholar
  45. Dankert, J., S-Gravenmade, E. J. &Wemes, J. C. (1976) Diffusion of formocresol and glutaraldehyde through dentin and cementum.J. Endodontics 2, 42–6.Google Scholar
  46. Davey, D. F. (1973) The effect of fixative tonicity on the myosin filament lattice volume of frog muscle fixed following exposure to normal or hypertonic Ringer.Histochem. J. 5, 87–104.Google Scholar
  47. De Bruijn, W. C. (1973) Glycogen, its chemistry and appearances in the electron microscope.J. Ultrastruct. Res. 42, 29–50.Google Scholar
  48. Descarries, L. &Dupin, J. C. (1974) Retention of noradrenaline-3H in brain and preferential extraction of labeled metabolites by glutaraldehyde fixation.Experientia 30, 1164–65.Google Scholar
  49. Dutt, M. K. (1974) Fixation of mammalian liver in concentrated neutral formaldehyde at different temperatures.Acta histochem. 51, 140–42.Google Scholar
  50. Ehrlich, P. &Lazarus, A. (1898)Die Angemie I. Wien: Äbt Holder.Google Scholar
  51. Eisenberg, B. R. &Mobley, B. A. (1975) Size changes in single muscle fibres during fixation and embedding.Tissue and Cell 7, 383–7.Google Scholar
  52. Eisenman, E. A. &Alfert, M. (1981) A new fixation procedure for preserving the ultrastructure of marine invertebrate tissues.J. Microsc. 125, 117–20.Google Scholar
  53. Elias, P. M. &Friend, D. S. (1975) The permeability barrier in mammalian epidermis.j. Cell Biol. 65, 180–91.Google Scholar
  54. Elling, F., Hasselager, E. &Friis, C. (1977) Perfusion fixation of kidneys in adult pigs for electron microscopy.Acta anat. 98, 340–2.Google Scholar
  55. Ellis, E. A. &Antony, D. W. (1979) A method for removing precipitate from ultrathin sections resulting from glutaraldehyde-osmium tetroxide fixation.Stain Technol. 54, 282–5.Google Scholar
  56. Emerman M. &Behrman, E. J. (1982) Cleavage and crosslinking of proteins with osmium (VIII) reagents.J. Histochem. Cytochem. 30, 395–7.Google Scholar
  57. Enerbäck, L. (1966) Mast cells in rat gastrointestinal mucosa. I. Effects of fixation.Acta path. microbiol. scand. 66, 303–12.Google Scholar
  58. Fahimi, H. D. &Cotran, R. S. (1971) Permeability studies in heat induced injury of skeletal muscle using lanthanum as a fine structural tracer.Am. J. Path. 62, 143–52.Google Scholar
  59. Ferguson, C. C. &Richardson, J. B. (1978) A simple technique for the utilization of postmortem tracheal and bronchial tissues for ultrastructural studies.Hum. Path. 9, 463–70.Google Scholar
  60. Figueroa, C. D. &Caorsi, I. (1980) Ultrastructural and morphometric study of Langerhan's cells in the normal human ectocervix.J. Anat. 131, 669–82.Google Scholar
  61. Fitzharris, T. P., Bloodgood, R. A. &McIntosh, J. R. (1972) The effect of fixation on the wave propagation of the protozoan axosytle.Tissue and Cell 4, 219–25.Google Scholar
  62. Forssmann, W. C., Ito, S., Weihe, E., Aoki, A., Dym, M. &Fawcett, D. W. (1979) An improved perfusion fixation method for the testis.Anat. Rec. 198, 307–14.Google Scholar
  63. Fowler, M. R. &McKeel, D. W. (1979) Human adenohypophysial quantitative histochemical cell classification. II. Evaluation of the effects of two fixatives and postmortem intervals.Arch. Path. lab. Med. 103, 621–3.Google Scholar
  64. Fraschini, A., Pellicciari, M., Biggiogera, M. &Romanini, M. G. M. (1981) The effect of different fixatives on chromatin: cytochemical and ultrastructural approaches.Histochem. J. 15, 763–79.Google Scholar
  65. Friend, D. S. &Murray, M. J. (1965) Osmium impregnation of the Golgi apparatus.Am. J. Anat. 117, 135–50.Google Scholar
  66. Frühling, J., Penasse, W., Sand, G. &Claude, A. (1971) Reactions de la digitonine avec le cholésterol et autres lipids de la cortico-surrénale du rat: étude par microscopie electronique.J. Micros. (Paris) 12, 83–106.Google Scholar
  67. Fujita, H., Komatsu, M. &Nakajima, T. (1978) Use of nitrogen mustard N-oxide for the fixation of electron microscopic tissues.Histochemistry 58, 49–56.Google Scholar
  68. Gahmberg, C. G., Virtanen, I. &Wartiovaara, J. (1978) Crosslinking of erythrocyte membrane proteins by periodate and intramembrane particle distribution.Biochem. J. 1, 683–6.Google Scholar
  69. Gershon, M. D. &Sherman, D. (1982) Select demonstration of serotonergic neurons and terminals in electron micrographs: loading with dihydroxytryptamine and fixation of NaMnO4.J. Histochem. Cytochem. 30, 769–73.Google Scholar
  70. Ghosh, B. K. &Nanninga, N. (1976) Polymorphism of the mesosome inBacillus licheniformis.J. Ultrastruct. Res. 56, 107–20.Google Scholar
  71. Gillette, W. B. (1975) Effect of postmortem tissue fixation on the tooth mobility and pocket depth in human beings.Oral Surg. 39, 130–4.Google Scholar
  72. Glauert, A. M. (1974) Fixation dehydration and embedding of biological specimens. InPractical Methods in Electron Microscopy. Vol. 3. (edited byGlauert, A. M.) Oxford: North Holland.Google Scholar
  73. Goldfischer, S., Kress, Y., Coltoff-Schiller, B. &Berman, J. (1981) Primary fixation in osmium-potassium ferrocyanide.J. Histochem. Cytochem. 29, 1105–11.Google Scholar
  74. Graem, N. &Helweg-Larsen, K. (1979) Mitotic activity and delay in fixation of human tissue.Acta path. microbiol. scand. Ser. A 87, 375–8.Google Scholar
  75. Gregory, G. E., Greenway, A. R. &Lord, K. A. (1980) Alcoholic Bouin fixation of insect nervous systems for Bodian silver staining. I. Composition of aged fixative.Stain Technol. 55, 143–9.Google Scholar
  76. Grzanna, R. (1982) Light microscopic immunocytochemistry with fixed unembedded tissues. InTechniques in Immunocytochemistry. Vol. 1. (edited byBullock, G. R. &Petrusz, P.). London: Academic Press.Google Scholar
  77. Heast, C. W. M., Kamp, D., Plasa, G. &Deuticke, B. (1977) Intra- and intermolecular crosslinking of membrane proteins in intact erythrocytes and ghosts by SH-oxidising agents.Biochim. biophys. Acta 469, 226–30.Google Scholar
  78. Hander, J. S., Romanovicz, D. K. &Padykula, H. A. (1976) Tissue fixation and osmium black formation with non-volatile octavalent osmium compounds.Histochemistry 49, 263–91.Google Scholar
  79. Hardonk, M. S., Haarsma, T. J., Dijkhuis, F. W. J., Poel, M. &Koudstaal, J. (1977) Influence of fixation and buffer treatment on the release of enzymes from the plasma membrane.Histochemistry 54, 57–66.Google Scholar
  80. Hardy, P. M., Hughes, G. J. & Rydon, H. N. (1976a) Formation of quaternary pyridinicum compounds by the action of glutaraldehyde on proteins.J. Chem. Soc. Chem. Commn. 157–60.Google Scholar
  81. Hardy, P. M., Nicholls, A. C. & Rydon, H. N. (1972) The hydration and polymerization of succinaldehyde, glutaraldehyde and adipaldehyde.J. Chem. Soc. (Perkin Transactions II) 2270–7.Google Scholar
  82. Hardy, P. M., Nicholls, A. C. &Rydon, H. N. (1976b) The nature of the cross-linking of proteins with glutaraldehyde. I. Interaction of glutaraldehyde with the amino groups of 6-amino hexonic acid and of α-N-acetyl-lysine,J. Chem. Soc. 1, 958–62.Google Scholar
  83. Hauser, M. (1978) Demonstration of membrane associated and orientated microfilaments inAmoeba proteus by means of a Schiff base/glutaraldehyde fixative.Cytobiol. 18, 95–106.Google Scholar
  84. Hassell, J. &Hand, A. R. (1974) Tissue fixation with diimidoesters as an alternative to aldehydes.J. Histochem. Cytochem. 22, 223–9.Google Scholar
  85. Hauser, M. (1978) Demonstration of membrane associated and orientated microfilaments in Amoeba proteus by means of a Schiff base/glutaraldehyde fixative.Cytobiol. 18, 95–106.Google Scholar
  86. Hayat, M. A. (1981)Fixation for electron microscopy. New York: Academic Press.Google Scholar
  87. Helander, H. F., Rehm, W. S. &Sanders, S. S. (1973) Influence of fixation on physiological properties of frog gastric mucosa.Acta physiol. scand. 88, 109–22.Google Scholar
  88. Herzog, V. & Fahimi, H. D. The effect of glutaraldehyde on catalase.J. Cell. Biol. 60, 303–11.Google Scholar
  89. Hillman, H. (1980) Artefacts in electron microscopy and the consequences for biological and medical research.Medical Hypotheses 6, 233–44.Google Scholar
  90. Hirsch, E. Z., Chisholm, G. M. &Gibbons, A. (1981) Quantitative assessment of changes in aortic dimensions in response toin situ perfusion fixation at physiological pressures.Atherosclerosis 38, 63–74.Google Scholar
  91. Hirsch, J. G. &Fedorko, M. E. (1968) Ultrastructure of human leukocytes after simultaneous fixation with gluraraldehyde and osmium tetroxide and postfixation in uranyl acetate.J. Cell. Biol. 38, 615–27.Google Scholar
  92. Hixson, D. C., Yep, J. M., Glenney, J. R., Hayes, T. &Walborg, E. F. (1981) Evaluation of periodate/lysine/paraformaldehyde fixation as a method for crosslinking plasma membrane glycoproteins.J. Histochem. Cytochem. 29, 561–6.Google Scholar
  93. Hoffstein, S., Gennaro, D. E., Fix, A. C., Hirsch, J., Streuli, F. &Weissmann, G. (1975) Colloidal lanthanum as a marker for impaired plasma membrane permeability in ischaemic dog myocardium.Am. J. Path. 79, 207–18.Google Scholar
  94. Hopwood, D. (1968) The effect of pH and various fixatives on isolated chromaffin granules with respect to the chromaffin reaction.J. Anat. 102, 415–24.Google Scholar
  95. Hopwood, D. (1970) The reactions between formaldehyde, glutaraldehyde and osmium tetroxide and their fixation effects on bovine serum albumin and tissue blocks.Histochemie 24, 56–64.Google Scholar
  96. Hopwood, D. (1972) Theoretical and practical aspects of glutaraldehyde fixation.Histochem. J. 4, 267–303.Google Scholar
  97. Hopwood, D. (1975) The reactions of glutaraldehyde with nucleic acids.Histochem. J. 7, 267–75.Google Scholar
  98. Hopwood, D. (1983) General principles of fixation. InHistochemistry in Pathology (edited byFilipe, M. I. &Lake, B. D.), pp. 1–11, Edinburgh: Churchill Livingstone.Google Scholar
  99. Hopwood, D., Allen, C. R. &McCabe, M. (1970) The reactions between glutaraldehyde and various proteins.Histochem. J. 2, 137–50.Google Scholar
  100. Hopwood, D., Coghill, G., Ramsay, J., Milne, G. &Kerr, M. (1984) Microwave fixation: its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy.Histochem. J. 16, 1171–91.Google Scholar
  101. Hopwood, D., Logan, K. R., Milne, G. &Bouchier, I. A. D. (1978) Concanavalin A receptors in normal and inflamed human oesophageal epithelium.Histochemistry 57, 255–63.Google Scholar
  102. Hopwood, D. &Mellors, A. E. (1972) An investigation of the chlorotriazine derivatives as fixatives for tissues.Histochem. J. 5, 391–403.Google Scholar
  103. Hopwood, D., Milne, G., Curtis, M. &Nicholson, G. (1979) The ionic components of normal human oesophageal epithelium.Histochemistry 64, 195–203.Google Scholar
  104. Hopwood, D., Milne, G., Khan, M. A., Crisp, M. &Walker, M. (1983) Anionic groups in basement membranes.Histochem. J. 15, 491–8.Google Scholar
  105. Howie, J. (1978)Code of practice for the prevention of infection in clinical laboratories and post mortem rooms. London: HMSO.Google Scholar
  106. Hunziker, E. B., Herrmann, W. &Schenk, R. K. (1982) Improved cartilage fixation by ruthenium hexamine trichloride (RHT).J. Ultrastruct. Res. 81, 1–12.Google Scholar
  107. Ionescu, M. I. (1979)Tissue heart valves. London: Butterworths.Google Scholar
  108. Iqbal, S. J. &Weakley, B. S. (1974) The effects of different preparative procedures on the ultrastructure of the hamster ovary.Histochemistry 38, 95–122.Google Scholar
  109. Ito, S. &Karnovsky, M. J. (1968) Formaldehyde—glutaraldehyde fixative containing trinitro compounds.J. Cell Biol. 39, 168–9a.Google Scholar
  110. Jansen, O. M. (1980) Cancer risk from formaldehyde.Lancet ii, 480–1.Google Scholar
  111. Johnson, W. D., Lang, M. &Johnson, M. T. (1978) Fixatives and methods of fixation in slected tissues of the laboratory rat.Clin. Toxicol. 12, 583–600.Google Scholar
  112. Jones, R. T. &Trump, B. F. (1975) Cellular and subcellular effects of ischaemia on the pancreatic acinar cell.Virchows Arch. B,19, 325–36.Google Scholar
  113. Joseph, S. A., Piekut, D. T. &Knigge, K. M. (1981) Immunocytochemical localisation of luteinising hormone releasing hormone (HRH) in vibratome sectioned brain.J. Histochem. Cytochem. 29, 247–54.Google Scholar
  114. Josephs, R., Eisenberg, H. &Reisler, E. (1973) Some properties of cross-linked polymers of glutaric dehydrogenase.Biochemistry 12, 4060–7.Google Scholar
  115. Jost, P., Brooks, V. J. &Griffith, D. H. (1973) Fluidity of phospholipid bilayers and membranes after exposure to osmium tetroxide and glutaraldehyde.J. molec. Biol. 76, 313–8.Google Scholar
  116. Kalimo, H. (1976) The role of the blood brain barrier in perfusion fixation of the brain for electron microscopy.Histochem. J. 8, 1–12.Google Scholar
  117. Kalimo, H., Garcia, J. H., Kamijyo, Y., Tanaka, J., Viloria, J. E., Valigorsky, J. M., Jones, R. T., Kim, K. M., Mergner, W. J., Pendergrass, R. E. &Trump, B. F. (1974) Cellular and subcellular alterations of human CNS.Arch. Path. 97, 352–9.Google Scholar
  118. Kalina, M. &Pease, D. C. (1977) The preservation of ultrastructure in saturated phosphatidyl cholines by tannic acid in model systems and type II pneumocytes.J. Cell Biol. 74, 726–41.Google Scholar
  119. Kanerva, L., Hervonen, A. &Rechardt, L. (1977) Permanganate fixation demonstrates the monoamine containing granular vesicles in the SIF cells but not in the adrenal medulla or mast cells.Histochemistry 52, 61–72.Google Scholar
  120. Kanwar, Y. S. &Farquhar, M. G. (1979), Anionic sites in the glomerular basement membrane.In vivo andin vitro localisation to the laminae rarae by cationic probes.J. Cell Biol. 81, 137–53.Google Scholar
  121. Karnovsky, M. J. (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. 11th Annual Meeting.Am. Soc. Cell Biol. p. 146.Google Scholar
  122. Kasten, F. H. &Lala, R. (1975) The Feulgen reaction after glutaraldehyde fixation.Stain Technol. 50, 199–201.Google Scholar
  123. Katsuyama, T. &Spicer, S. S. (1977) Ionic components of secretory cell surfaces in relation to secretory function.Histochem. J. 9, 467–94.Google Scholar
  124. Kiernan, J. A. (1975) Localisation of α-D-glycosyl and α-D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase.Histochemistry 44, 39–45.Google Scholar
  125. Kirschner, D. A. &Hollingshead, C. J. (1980) Processing for electron microscopy alters membrane structure and packing in myelin.J. Ultrastruct. Res. 73, 211–32.Google Scholar
  126. Knoll, J. S. &Fluck, R. A. (1978) Improved fixation for primitive erythroid cells from chick embryo.Stain Technol. 53, 118–9.Google Scholar
  127. Koga, A., Todd, S. &Nishimura, M. (1975) Electron microscopic observations on the cholesterol distributed in the epithelial cells of the gallbladder.Histochemistry 44, 303–6.Google Scholar
  128. Kolb-Bachofen, V. (1977) Electron microscopic localisation of acid phosphatase inTetrahymena pyriformis.Cytobiology 15, 135–44.Google Scholar
  129. Komnick, H. (1962) Electronmikroskopische lokalisation von Na+ und Q in Zellen und Geweben.Protoplasma 55, 414.Google Scholar
  130. Komorowska, M., Koter, M., Bartosz, G. &Glomulkiewicz, J. (1982). The effects of glutaraldehyde and osmium tetroxide on the erythrocyte membrane.Biochim. biophys. Acta 686, 94–8.Google Scholar
  131. Korn, A. H., Feairheller, S. H. &Filachione, E. M. (1972) Glutaraldehyde — Nature of the reagent.J. molec. Biol. 65, 525–60.Google Scholar
  132. Krawczyk, W. S. (1976) Ultrastructural studies on the external surface coat of epidermal cells.Arch. Dermatol. Res. 255, 157–62.Google Scholar
  133. Kruth, H. S., Blanchette-Mackie, J., Avigan, W. &Vaughan, M. (1982) Subcellular localization and quantitation of cholesterol in cultured fibroblasts exposed to human low density lipoproteins.J. Lipid Res. 23, 1128–35.Google Scholar
  134. Kuypers, G. A. J. &Roomans, G. M. (1980) Post mortem elemental redistribution in rat studied by X-ray microanalysis and electron microscopy.Histochemistry 69, 145–56.Google Scholar
  135. La Fontaine, J. R., Zobel, C. R., Thomas, H. R. &Galbreath, C. (1977) Fixation and staining of F-actin and microfilaments using tannic acid.J. Ultrastruct. Res. 58, 78–86.Google Scholar
  136. Lake, B. D. &Ellis, R. B. (1976) What do you think you are quantifying? An appraisal of histochemical methods in the measurement of the activities of lysosomal enzymes.Histochem. J. 8, 357–66.Google Scholar
  137. Lancet (1983) Formaldehyde and cancer.2, 26.Google Scholar
  138. Landis, W. J., Paine, M. C. &Glimcher, M. J. (1980) Use of acrolein vapors for the anhydrous preparation of bone tissue for electron microscopy.J. Ultrastruct. Res. 70, 171–80.Google Scholar
  139. Landmann, L. (1980) Lamellar granules in mamalian, avian and reptilian epidermis.J. Ultrastruct. Res. 72, 245–63.Google Scholar
  140. Langenberg, W. G. (1978) Chromic acid formaldehyde fixation of nucleic acids of bacteriophage ø6 and infectious rhinotracheitis virus.J. Gen. Virol. 39, 377–80.Google Scholar
  141. Langenberg, W. G. (1979) Chilling of tissue before glutaraldehyde fixation preserves fragile inclusions of several plant viruses.J. Ultrastruct. Res. 66, 120–31.Google Scholar
  142. Langenberg, W. G. (1980) Glutaraldehyde nonfixation of isolated viral and yeast RNAs.J. Histochem. Cytochem. 28, 311–5.Google Scholar
  143. Langford, L. A. &Coggeshall, R. E. (1980) The use of potassium ferricyanide in neural fixation.Anat. Rec. 197, 297–303.Google Scholar
  144. Lansdown, A. B. G. &Ellaby, S. J. (1974) Formaldehyde chloralhydrate as a fixative for mouse tissues for localisation of acid and alkaline phosphatases.Histochemistry 42, 111–3.Google Scholar
  145. Lapis, K. &Timar, J. (1980) Cytochemical studies on the glycosaminoglycan components of the cell coat.Biol. Cellulaire 39, 202–12.Google Scholar
  146. Larsen, W. J. (1975) Opaque deposits on gap junction membranes after glutaraldehyde calcium fixation.J. Cell Biol. 67, 801–13.Google Scholar
  147. Larsson, L. (1975) Effects of different fixatives on the ultrastructure of the developing proximal tubule of rat kidney.J. Ultrastruct. Res. 51, 140–51.Google Scholar
  148. Laurila, P., Virtanen, I., Wartiovaara, J. &Stenman, S. (1978) Fluorescent antibodies and lectins stain intracellular structures in fixed cells treated with non-ionic detergent.J. Histochem. Cytochem. 26, 251–7.Google Scholar
  149. Laver, J. L., Van Drunen, N. A., Washburn, J. W. &Balfour, H. H. (1979) Transmission of hepatitis B virus in clinical laboratory areas.J. Infect. Dis. 140, 513–76.Google Scholar
  150. Lee, R. M. K. V., McKenzie, R., Kobayashi, K., Garfield, R. E., Forrest, J. B. &Daniel, E. E. (1981) Effects of glutaraldehyde fixative osmolarities on smooth muscle cell volume and osmotic reactivity of the cells after fixation.J. Microsc. 125, 77–88.Google Scholar
  151. Lehir, M., Herzog, V. &Fahimi, H. D. (1979) cytochemical detection of catalase with 3,3′-diaminobenzidine.Histochemistry 64, 51–66.Google Scholar
  152. Leong, S. P. L., Cooperband, S. R., Deckers, P. J., Sutherland, C. M., Cesare, J. F. &Krementzet, E. T. (1979) Effect of different fixatives on the localization of human melanoma antigens by immunofluorescence.Oncology 36, 202–7.Google Scholar
  153. Levison, D. A., Pennington, C. R. &Hopwood, D. (1977) An ultrastructural study of osmiophilia in the human rectum.Histochem. J. 9, 77–89.Google Scholar
  154. Lillie, R. D. &Fullmer, H. M. (1976)Histopathologic Technique and Practical Histochemistry. London: McGraw Hill.Google Scholar
  155. Lindberg, L. G. &Ohlin, B. (1978) Specimen fixation in urinary cytology.Acta cytol. 22, 142–5.Google Scholar
  156. Logan, K. R., Milne, G. &Hopwood, D. (1977) An ultrastructural study of osmiophilia in normal human oesophageal e[ithelium.Histochemistry 53, 183–7.Google Scholar
  157. Login, G. R. (1978) Microwave fixation versus formalin fixation of surgical and autopsy tissue.Am. J. med. Technol. 44, 435–7.Google Scholar
  158. Loomis, T. A. (1979) Formaldehyde toxicity.Arch. Path. Lab. Med. 102, 312–4.Google Scholar
  159. Luft, J. F. (1976) The structure and properties of the cell surface coat.Int. Rev. Cytol. 45, 291–382.Google Scholar
  160. Luft, J. H. (1971) Ruthenium Red and Violet. II. Fine structural localisation in animal tissues.Anat. Rec. 171, 369–416.Google Scholar
  161. Luftig, R. B. &Macmillan, P. N. (1981) The importance of adequate fixation in preservation of membrane ultrastructure.Int. Rev. Cytol. Suppl.12, 309–25.Google Scholar
  162. Luftig, R. B., McMillan, P. N., Weatherbee, J. A. &Weihing, R. R. (1977) Increased visualization of microtubules by an improved fixation procedure.J. Histochem. Cytochem. 25, 175–87.Google Scholar
  163. Lust, W. D., Veech, R. L. &Passoneau, J. V. (1973) Cyclic adenosine monophosphate metabolites and phosphocyclase in neural tissue: a comparison of methods of fixation.Science 181, 280–2.Google Scholar
  164. McClung, R. E. &Wood, J. (1982) Analytical electron microscopic evaluation of the effects of paraformaldehyde pretreatment on the reaction of glutaraldehyde with biogenic amines.J. Histochem. Cytochem. 30, 481–6.Google Scholar
  165. McDowell, E. M. &Trump, B. J. (1976) Histologic fixatives suitable for diagnostic light and electron microscopy.Arch. Path. Lab. Med. 100, 405–44.Google Scholar
  166. McGhee, J. D. &Von Hippel, P. H. (1976a) Formaldehyde as a probe of DNA structure. 3. Equilibrium denaturation of DNA and synthetic polynucleotides.Biochemistry 16, 3267–76.Google Scholar
  167. McGhee, J. D. &Von Hippel, P. H. (1976b) Formaldehyde as a probe of DNA structure. 4. Mechanisms of the initial reaction of formaldehyde with DNA.Biochemistry 16, 3276–93.Google Scholar
  168. McLean, I. W. &Nakane, P. K. (1974) Periodate-lysine-paraformaldehyde fixative.J. Histochem. Cytochem. 22, 1077–85.Google Scholar
  169. Mann, D. M. A., Barton, C. M. &Davies, J. S. (1978) Postmortem changes in human central nervous tissue and the effects on quantitation of nucleic acids and enzymes.Histochem. J. 10, 127–36.Google Scholar
  170. Markarian, B. (1975) A simple method of inflation fixation and air drying of lungs.Am. J. clin. Path. 63, 20–4.Google Scholar
  171. Mascorro, J. A., Yates, R. D. &Chen, I. L. (1976) A glutaraldehyde/potassium dichromate tracing method for the localization and preservation of abdominal extra adrenal chromaffin tissues.Stain Technol. 50, 391–6.Google Scholar
  172. Mathieu, O., Claassen, H. &Weibel, E. R. (1978) Differential effect of buffer and glutaraldehyde osmolarity on cell dimensions. A study on lung tissue.J. Ultrastruct. Res. 63, 20–34.Google Scholar
  173. Maupin-Szamier, P. &Pollard, T. D. (1978) Actin filament destruction by osmium tetroxide.J. Cell Biol. 77, 837–52.Google Scholar
  174. Mayers, C. P. (1970) Histological fixation by microwave heating.J. clin. Path. 23, 273–5.Google Scholar
  175. Meek, K. M. (1981) The use of glutaraldehyde and tannic acid to preserve reconstituted collagen for electron microscopy.Histochemistry 73, 115–20.Google Scholar
  176. Meek, K. M. &Weiss, I. B. (1979) Differential fixation of poly(l-arginine) and poly(l-lysine) by tannic acid and its application to the fixation of collagen in electron microscopy.Biochim. biophys. Acta 587, 112–20.Google Scholar
  177. Meijer, A. E. F. H. &Israel, D. E. (1978) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques.Histochemistry 57, 9–22.Google Scholar
  178. Mersey, B. &McCulley, M. E. (1978) Monitoring of the course of fixation of plant cells.J. Microsc. 114, 49–76.Google Scholar
  179. Mizuhira, V. &Futaesaku, Y. (1972) New fixation for biological membranes using tannic acid.Acta histochem. cytochem. 5, 233–5.Google Scholar
  180. Mizuhira, V., Shiihashi, M. &Futaesaku, Y. (1981) High speed electron microscope autoradiographic studies of diffusible compounds.J. Histochem. Cytochem. 29, 143–60.Google Scholar
  181. Molin, S. O., Nygren, H. &Dolonius, L. (1978a) A new method for the study of glutaraldehyde induced crosslinking properties in proteins with special reference to the reaction with amino groups.J. Histochem. Cytochem. 26, 412–4.Google Scholar
  182. Molin, S. O., Nygren, H., Dolonius, L. &Hansson, H. A. (1978b) A kinetic study of the reaction between glutaraldehyde and horseradish peroxidase.J. Histochem. Cytochem. 26, 1053–6.Google Scholar
  183. Molin, S. G., Nygren, H. &Hansson, H. A. (1978c) Binding of glutaraldehyde reacted peroxidase to cell surfaces.J. Histochem. Cytochem. 26, 325–6.Google Scholar
  184. Mollenhauer, H. H., Morre, D. J. &Hass, B. S. (1979) Plasma membrane transformations in spermatogenesis revealed by aldehyde fixatives containing tannic acid.J. Ultrastruct. Res. 61, 166–71.Google Scholar
  185. Møller, J. C., Skriver, E., Olsen, S. &Maunsbach, A. B. (1982) Perfusion fixation of human kidneys for ultrastructural analysis.Ultrastruct. Path. 3, 375–85.Google Scholar
  186. Monsan, P., Puzo, G. &Mazarguil, H. (1975) Etude du mécanisme d'établissement des liasons glutaraldehyde-protéins.Biochemie 57, 1281–92.Google Scholar
  187. Moran, D. &Rice, R. W. (1975) An ultrastructural examination of the role of cell membrane surface coat material during neurulation.J. Cell. Biol. 64, 172–81.Google Scholar
  188. Mortensen, N. J. M. &Morris, J. F. (1977) The effect of fixation conditions on the ultrastructural appearance of gastric cell granules in the rat gastric pyloric antrum.Cell Tiss. Res. 176, 251–63.Google Scholar
  189. Mueller, W. C. &Greenwood, A. D. (1978) The ultrastructure of phenolic storing cells fixed with caffein.J. exp. Botany 29, 757–64.Google Scholar
  190. Müller, P., Raabe, G. &Schumann, D. (1978) Leukoplakia induced by repeated deposition of formalin in rabbit oral mucosa.Exp. Path. (Jena) 16, 36–42.Google Scholar
  191. Munz, K. &Meves, C. (1974) Digestibility of elastic fibres by elastase in semifine and fine sections as a function of fixation mode and embedding media.Histochemistry 40, 181–90.Google Scholar
  192. Nathanson, J. A. (1977) Cyclic nucleotides and nervous system function.Physiol. Rev. 57, 157–256.Google Scholar
  193. Nevalainen, T. J. &Anttinen, J. (1977) Ultrastructural and functional changes in pancreatic acinar cells during autolysis.Virchows Arch. B,24, 197–207.Google Scholar
  194. Ni, C., Chang, T. C., Searl, S. S., Coughlin-Wilkinson, E. &Albert, D. M. (1981) Rapid paraffin fixation for use in histologic examinations.Ophthalmol. (Rochester) 88, 1372–6.Google Scholar
  195. Nielson, A. J. &Griffith, W. P. (1978) Tissue fixation and staining with osmium tetroxide: the role of phenolic compounds.J. Histochem. Cytochem. 26, 138–40.Google Scholar
  196. Nielson, A. J. &Griffith, W. P. (1979) Tissue fixation by osmium tetroxide.J. Histochem. Cytochem. 37, 997–9.Google Scholar
  197. Nir, I. &Hall, M. O. (1974) The ultrastructure of lipid depleted rod photoreceptor membranes.J. Cell Biol. 63, 587–98.Google Scholar
  198. Nunez-Duran, H. (1980) Tannic acid as an electron microscope tracer for permeable cell membranes.Stain Technol. 55, 361–5.Google Scholar
  199. O'Brien, T. P., Kuo, J., McKully, M. E. &Zee, S. Y. (1973) Coagulant and non-coagulant fixation of plant cells.Aust. J. biol. Sci. 26, 1231–50.Google Scholar
  200. O'Hare, K. O. &Braunschweig, R. J. (1975) The effects of various fixative buffer combinations on lung fine structure.Anat. Rec. 181, 545–60.Google Scholar
  201. Ohnishi, A., Offord, K. &Dyck, P. J. (1974a) Studies to improve fixation of human nerves. I. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry.J. Neurol. Sci. 23, 223–6.Google Scholar
  202. Ohnishi, A., O'Brien, P. &Dyck, P. J. (1974b) Studies to improve fixation of human nerves. 2. The effect of time elapsed between death and glutaraldehyde fixation on relationship of axonal area to number of myelin lamellae.J. Neurol. Sci. 23, 387–90.Google Scholar
  203. Oi, T. &Utsumi, N. (1980) Ultrastructure of hypertrophic chondrocytes of rat mandibular condyles using lanthanum containing fixatives.Arch. Oral. Biol. 25, 77–81.Google Scholar
  204. Olins, D. E. &Wright, E. B. (1973) Glutaraldehyde fixation of isolated eucaryotic nuclei.J. Cell Biol. 59, 304–17.Google Scholar
  205. Ortner, M. J., Galvin, M. J., Chignell, C. F. &McRee, D. I. (1981) A circular dichroism study of human erythrocyte ghost proteins during exposure to 2450 mHz microwave radiation.Cell Biophys. 3, 335–47.Google Scholar
  206. Oschman, J. L., Hall, T. A., Peters, P. D. &Wall B. J. (1974) Association of calcium with membranes of squid giant axon.J. Cell Biol. 61, 156–65.Google Scholar
  207. Paljärvi, L., Garcia, J. H. &Kalimo, H. (1979) The efficiency of aldehyde fixation for electron microscopy: stabilization of rat brain tissue to withstand osmotic stress.Histochem. J. 11, 267–76.Google Scholar
  208. Patterson, M. K. &Bulard, R. (1980) Microwave fixation of cells in tissue culture.Stain Technol. 55, 71–5.Google Scholar
  209. Paula-Barbosa, M. &Gray, E. C. (1974) The effects of various fixatives at different pH on synaptic coated vesicles, reticulosomes and cytonet.J. Neurocytol. 3, 471–86.Google Scholar
  210. Pearse, A. G. E. (1980)Histochemistry Theoretical and Applied. 4th edn. Vol. 1. Edinburgh: Churchill Livingstone.Google Scholar
  211. Pearse, A. G. E. &Polak, J. M. (1975) Bifunctional reagents as vapour and liquid phase fixatives for immunohistochemistry.Histochem. J. 7, 179–86.Google Scholar
  212. Pelttari, A. &Helminen, H. J. (1979) The effects of various fixatives on the relative thickness of the cellular membranes of the central lobe of the rat prostate.Histochem. J. 11, 599–611.Google Scholar
  213. Penttila, A., McDowell, E. M. &Trump, B. F. (1975) Effects of fixation and postfixation treatments on volume of injured cells.J. Histochem. Cytochem. 23, 251–70.Google Scholar
  214. Peracchia C. &Mittler, B. S. (1972) New glutaraldehyde fixation procedures.J. Ultrastruct. Res. 39, 57–64.Google Scholar
  215. Persson, L. (1976) Cellular reaction to small cerebral stab wounds.Virchows Arch B22, 21–37.Google Scholar
  216. Peters, K. &Richards, F. M. (1977) Chemical crosslinking: reagents and problems in studies of membrane structure.A. Rev. Biochem. 46, 523–51.Google Scholar
  217. Petersen, P. (1977) Glutaraldehyde fixation for electron microscopy of needle biopsies of human livers.Acta path. microbiol. scand., Ser. A 85, 373–83.Google Scholar
  218. Petrere, J. A. &Schardein, J. L. (1977) Microwave fixation of fetal specimens.Stain Technol. 52, 113–4.Google Scholar
  219. Phillips, E. R., Kletzien, R. F. &Pedue, J. F. (1977) A supravital polyaldehyde fixative for external cell surfaces.Exp. Cell Res. 105, 51–62.Google Scholar
  220. Pousty, I., Bari-Khan, M. A. &Butler, W. F. (1975) Leaching of glycosaminoglycans from tissues b the fixatives formalin-saline and formalin-cetrimide.Histochem. J. 7, 361–5.Google Scholar
  221. Prosperi, E. &Raap, A. K. (1982) Substrate protection during the fixation of β-glucuronidase.Histochem. J. 14, 689–95.Google Scholar
  222. Radmehr, B. &Butler, W. F. (1978) Leaching of glycosaminoglycan from cow uterus and vagina into fixative solution.Histochem. J. 10, 465–8.Google Scholar
  223. Rambourg, A., Clermont, Y. &Marnaud, A. (1974) Three dimensional structure of osmium impregnated Golgi apparatus as seen in high voltage electron microscopy.Am. J. Anat. 140, 27–46.Google Scholar
  224. Rasmussen, K. E. &Albrechtsen, J. (1974) Glutaraldehyde. The influence of pH temperature and buffering on the polymerisation rate.Histochemistry 38, 19–26.Google Scholar
  225. Rechardt, L., Kanerva, L. &Hervonen, H. (1977) Ultrastructural demonstration of amine granules in the adrenal medullary cells of the rat using acid permanganate.Histochemistry 54, 339–43.Google Scholar
  226. Renau-Piqueras, J., Knecht, E. &Hernandez-Yago, J. (1981) Effects of different fixative solutions on labeling of concanavalin A receptor sites in human T-lymphocytes.Histochemistry 71, 559–65.Google Scholar
  227. Revel, J. P. &Karnovsky, M. J. (1967) Hexagonal array of subunits in intracellular junctions of the mouse heart and liver.J. Cell. Biol. 33, 7–12.Google Scholar
  228. Richardson, K. (1966) Electron microscopic identification of autonomic nerve endings.Nature, Lond. 210, 756.Google Scholar
  229. Robinson, J. M. &Karnovsky, M. J. (1980) Evaluation of polyene antibiotic filipin as a cytochemical probe for membrane cholesterol.J. Histochem. Cytochem. 28, 161–8.Google Scholar
  230. Roomans, G. M. (1975) Tannic acid fixation of human spermatozoa.J. Reprod. Text. 43, 197–200.Google Scholar
  231. Rosene, D. L. &Mesulam, M. M. (1978) Fixation variables in horseradish peroxidase neurochemistry.J. Histochem. Cytochem. 26, 28–39.Google Scholar
  232. Rostgaard, J. &Tranum-Jensen, J. (1980) A procedure for minimizing cellular shrinkage in electron microscope preparation: a quantitative study on frog gallbladder.J. Microsc. 119, 213–32.Google Scholar
  233. Russell, A. D. &Hopwood, D. (1976) The biological uses and improtance of glutaraldehyde.Prog. Med. Chem. 13, 271–302.Google Scholar
  234. Saito, T. &Keino, H. (1976) Acrolein as a fixative for enzyme histochemistry.J. Histochem. Cytochem 24, 1258–69.Google Scholar
  235. Sannes, L. P., Katsuyama, T. &Spicer, S. S. (1978) Tannic acid-metal salt sequences for light and electron microscopic localization of complex carbohydrates.J. Histochem. Cytochem. 26, 55–61.Google Scholar
  236. Santos-Sacchi, J. (1978) Differential effects of primary fixation with glutaraldehyde and osmium upon the membranous systems of the strial and external sulcus cells.Acta otolaryngol. 86, 56–63.Google Scholar
  237. Schmidt, D. E., Speth, R. C., Welsch, F. &Schmidt, M. J. (1972) The use of microwave radiation in the determination of acetyl-choline in the rat brain.Brain Res. 38, 377–89.Google Scholar
  238. Schneider, G. B. (1976) The effects of preparative procedures for scanning electron microscopy on the size of isolated lymphocytes.Am. J. Anat. 146, 93–100.Google Scholar
  239. Schneider, E. M. &Sievers, A. (1981) Concanavalin A binds to the endoplasmic reticulum and the starch grain surface of root statocytes.Planta 152, 177–80.Google Scholar
  240. Schulte, B. A. &Spicer, S. S. (1983) Light microscopic histochemical detection of terminal galactose andN-acetylgalactosamine residues in rodent complex carbohydrates using a galactose and oxidase-Schiff peanut lectin horseradish peroxidase conjugate.J. Histochem. Cytochem. 31, 19–24.Google Scholar
  241. Schwetje, N. &Thiessen, G. (1979) The development of a new technology for controlled cell fixation. A methodological pilot study.J. Histochem. Cytochem. 27, 3–6.Google Scholar
  242. Scott, J. E. (1980) Collagen proteoglycan interactions. Localization of proteoglycans by electron microscopy.Biochem. J. 187, 887–91.Google Scholar
  243. Senior, M. B. &Olins, D. E. (1975) Effect of formaldehyde on the circular dichroism of chicken erythrocyte chromatin.Biochemistry 14, 3332–7.Google Scholar
  244. Shea, S. M. (1971) Lanthanum staining of the surface coat of cells.J. Cell Biol. 51, 611–20.Google Scholar
  245. Shephard, N. &Mitchell, N. (1976a) The localization of proteoglycan by light and electron microscopy using Saffranin O.J. Ultrastruct. Res. 54, 457–60.Google Scholar
  246. Shephard, N. &Mitchell, N. (1976b) Simultaneous localization of proteoglycan by light and electron microscopy using Toluidine blue O.J. Histochem. Cytochem. 24, 621–9.Google Scholar
  247. Shiga, T., Suda, T. &Maeda, N. (1977) Spin label studies on the human erythrocyte membrane.Biochim. biophys. Acta 466, 231–44.Google Scholar
  248. Simionescu, N. &Simionescu, M. (1976a) Galloyl glucoses of low molecular weight as mordant in electron microscopy. 1. Procedure and evidence for mordanting effect.J. Cell Biol. 70, 608–21.Google Scholar
  249. Simionescu, N. &Simionescu, M. (1976b) Galloyl glucoses of low molecular weight as mordant in electron microscopy. II. The moiety and functional groups possibly involved in the mordanting effect.J. Cell Biol. 70, 622–33.Google Scholar
  250. Simson, J. A. V. &Spicer, S. S. (1975) Selective subcellular localization of cations with variants of the potassium (pyro) antimonate technique.J. Histochem. Cytochem. 23, 575–98.Google Scholar
  251. Simson, J. A. V., Spicer, S. S. &Hall, B. J. (1974) Morphology and cytochemistry of rat salivary gland acinar secretory granules and their alteration by isoproterenol.J. Ultrastruct. Res. 48, 465–82.Google Scholar
  252. Skaer, R. J. &Whytock, S. (1977) Chromatin-like artefacts from nuclear sap.J. Cell Sci. 26, 301–10.Google Scholar
  253. Sommer, J. R., Dolber, P. C. &Taylor, I. (1980) Filipin-cholesterol complexes in the sarcoplasmic reticulum of frog skeletal muscle.J. Ultrastruct. Res. 72, 272–85.Google Scholar
  254. Sorvari, T. E. &Lauren, P. A. (1973) The effect of various fixation procedures on the digestability of sialomucins with neuraminidase.Histochem. J. 5, 405–12.Google Scholar
  255. Squier, C. A., Waterhouse, J. P. &Kraucunas, E. (1975) The application of stereological methods for studying the effects on differing osmolalities on the intercellular space of oral epithelium II. Inflamed Epithelium.J. Oral Path. 2, 136–41.Google Scholar
  256. Stefanini, M., De Martino, C. &Zamboni L. (1967) Fixation of ejaculated spermatozoa for electron microscopy.Nature, Lond. 216, 173–4.Google Scholar
  257. Stickland, N. C. (1975) A detailed analysis of the effects of various fixatives on animal tissue with particular reference to muscle tissue.Stain Technol. 50, 255–64.Google Scholar
  258. Stoward, P. J. (1973)Fixation in Histochemistry (edited byStoward, P. J.) London: Chapman and Hall.Google Scholar
  259. Strobel, S., Miller, H. R. P. &Ferguson, A. (1981) Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.J. clin. Path. 34, 851–8.Google Scholar
  260. Tachibana, T. (1975) Comparative ultrastructural histochemistry of the adepidermal granules ofSalmo irideus, Lebistes reticulatus andHynobius tokyocusis.J. Histochem. Cytochem. 23, 289–94.Google Scholar
  261. Takahashi, M., Suzuki, H. &Yamamoto, I. (1978) A formaldehyde-osmium tetroxide mixture and a formaldehyde-glutaraldehyde-osmium tetroxide mixture as fixative for electron microscopy.J. Electron Microsc. 27, 149–51.Google Scholar
  262. Takeuchi, K. I. (1980) Lipid droplets in the visceral yolk sac endodermal cells of the post implantation rat embryo: application of Malachite green-glutaraldehyde fixative.Cell Tiss. Res. 209, 29–41.Google Scholar
  263. Taylor, I. W. &Milthorpe, B. K. (1980) An evaluation of DNA fluorochromes staining techniques and analysis for flow cytometry.J. Histochem. Cytochem. 28, 1224–32.Google Scholar
  264. Teichman, R. J., Cummins, J. M. &Takei, G. H. (1974) The characterisation of a Malachite green stainable, glutaraldehyde extractable phospholipid in rabbit spermatozoa.Biol. Reprod. 10, 565–77.Google Scholar
  265. Thureson-Klein, A., Klein, R. L. &Yen, S. C. (1975) Morphological effects of osmolarity on purified noradrenergic vesicles.J. Neurocytol. 4, 609–27.Google Scholar
  266. Thurston, R. J., Hess, R. A., Kilburn, K. H. &McKenzie, W. N. (1976) Ultrastructure of lungs fixed in inflation using a new osmium-fluorocarbon technique.J. Ultrastruct. Res. 56, 39–47.Google Scholar
  267. Timar, J., Gyapay, G. &Lapis, K. (1979) Acridine orange staining of the mammalian fibroblast cell coat.Histochemistry 64, 189–93.Google Scholar
  268. Tisdale, A. D. &Nakajima, Y. (1976) Fine structure of synaptic vesicles in two types of nerve terminals in crayfish receptor organs: influence of fixation methods.J. comp. Neurol. 165, 369–86.Google Scholar
  269. Tixier-Vidal, A. &Picart, R. (1971) Electron microscopic localisation of glycoproteins in pituitary cells of duck and quail.J. Histochem. Cytochem. 19, 775–97.Google Scholar
  270. Traganos, F., Darzynkiewicz, Z., Sharpless, T. &Melamed, M. R. (1975) Denaturation of deoxyribonucleic acidin situ. Effect of formaldehyde.J. Histochem. Cytochem. 23, 431–8.Google Scholar
  271. Tzeng, S., Fukuyama, K. &Epstein, W. L. (1981) Effects of glutaraldehyde and osmium tetroxide on newborn rat epidermal keratin.J. Histochem. Cytochem. 29, 351–6.Google Scholar
  272. Vaccaro, A. &Brody, J. S. (1979) Ultrastructural localisation and characterisation of proteoglycans in the pulmonary alveolus.Am. Rev. Resp. Dis. 120, 901–10.Google Scholar
  273. Van Deurs, B. (1975) The use of a tannic acid-glutaraldehyde fixative to visualise gap and tight junctions.J. Ultrastruct. Res. 50, 185–92.Google Scholar
  274. Van Deurs, B. &Luft, J. H. (1979) Effect of glutaraldehyde fixation on the structure of tight junctions.J. Ultrastruct. Res. 68, 160–72.Google Scholar
  275. Van Prooijen-Knegt, A. C., Redi, C. A. &Van Der Ploeg, M. (1980) Quantitative aspects of the cytochemical Feulgen-DNA procedure studied on model systems and cell nuclei.Histochemistry 69, 1–17.Google Scholar
  276. Varricchio, F. &Jamieson, G. (1977) Reactivity of heterogeneous F1 histones with glutaraldehyde and formaldehyde.Exp. Cell Res. 106, 380–6.Google Scholar
  277. Vassar, P. S., Hards, J. M., Brooks, D. E., Hagenberger, B. &Seaman, G. V. F. (1972) Physicochemical effects of aldehydes on the human erythrocyte.J. Cell Biol. 53, 809–18.Google Scholar
  278. Vermeer, B. J., Van Gent, C. M., De Bruijn, W. C. &Boonders, T. (1978) The effect of digitonin containing fixatives on the retention of free cholesterol and cholesterol esters.Histochem. J. 10, 287–98.Google Scholar
  279. Vrensen, G. &De Groot, D. (1974) Osmium-zinc iodide staining and the quantitative study of central synapses.Brain Res. 74, 131–42.Google Scholar
  280. Wagner, R. C. (1976) The effect of tannic acid on electron images of capillary endothelial cell membranes.J. Ultrastruct. Res. 57, 132–9.Google Scholar
  281. Wakabayashi, T., Asano, M., Kuruno, C. &Kimura, H. (1975) Effects of extremely low concentrations of glutaraldehyde together with shortening of fixation times on the maintenance of various enzyme activities.J. Histochem. Cytochem. 23, 632–4.Google Scholar
  282. Ward, B. J. &Gloster, J. A. (1976) Lipid losses during processing of cardiac muscle for electron microscopy.J. Microsc. 108, 41–50.Google Scholar
  283. Weakley, B. S. (1974) A comparison of three different electron microscopical grade glutaraldehydes used to fix ovarian tissue.J. Microsc. 101, 127–41.Google Scholar
  284. Weakley, B. S. (1981)A Beginner's Handbook in Biological Transmission Electron Microscopy. 2nd edition. Edinburgh: Churchill Livingstone.Google Scholar
  285. White, D. L., Andrews, S. B., Faller, J. W. &Barrnett, R. J. (1976) The chemical nature of osmium tetroxide fixation and staining of membranes by X-ray photoelectron microscopy.Biochim. biophys. Acta 436, 577–92.Google Scholar
  286. Wick, S. M. &Hepler, P. K. (1982) Selective localisation of intracellular Ca2+ with potassium antimonate.J. Histochem. Cytochem. 30, 1190–204.Google Scholar
  287. Willingham, M. C. &Yamada, S. S. (1979) Development of a new primary fixation for electron microscope immunocytochemical localisation of intracellular antigens in cultured cells.J. Histochem. Cytochem. 27, 947–60.Google Scholar
  288. Wilson, N. H. F. &Gardner, D. L. (1980) Influence of aqueous fixation on articular surface morphology. I Little known effects of fixatives on morphology of articular surfaces.J. Path. 131, 333–8.Google Scholar
  289. Winborn, W. B. &Seelig, L. L. (1974) Pattern of osmium deposition in the parietal cells of the stomach.J. Cell Biol. 63, 99–108.Google Scholar
  290. Wood, J. G. (1973) The effects of glutaraldehyde and osmium on the proteins and lipids of myelin and mitochondria.Biochim. biophys. Acta 329, 118–27.Google Scholar
  291. Woodruff, E. A. (1979) The chemistry and biology of aldehyde treated tissue heart valve xenografts. InTissue Heart Valves (edited byIonescu, M. I.), pp. 347–63. London: Butterworths.Google Scholar
  292. Wright, B. M., Slavin, G., Kreel, L., Callan, K. &Sandin, B. (1975) Postmortem inflation and fixation of human lungs.Thorax 29, 189–94.Google Scholar
  293. Yang, J. &Scholten, T. (1977) A fixative for intestinal parasites permitting the use of concentration and permanent staining procedures.Am. J. clin. Path. 67, 300–4.Google Scholar
  294. Yarom, R., Peters, P. D. &Hall, T. A. (1974) Effect of glutaraldehyde and urea embedding on intracellular ionic elements.J. Ultrastruct. Res. 49, 405–18.Google Scholar
  295. Yokota, S. &Fahimi, H. D. (1978) The peroxisome (microbody) membrane: effects of detergents and lipid solvents on its ultrastructure and permeability to catalase.Histochem. J. 10, 469–87.Google Scholar
  296. Zalokar, M. &Erk, I. (1977) Phase partition fixation and staining of Drosophila eggs.Stain Technol. 52, 89–95.Google Scholar
  297. Zeikus, J. A. &Aldrich, H. C. (1975) Use of hot formaldehyde fixatives in processing plant parasitic nematodes for electron microscopy.Stain Technol. 50, 219–25.Google Scholar

Copyright information

© Chapman and Hall Ltd. 1985

Authors and Affiliations

  • D. Hopwood
    • 1
  1. 1.Department of PathologyNinewells Hospital and Medical SchoolDundeeUK

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