Skip to main content
SpringerLink
Log in

Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-Fast Black salt K: a method for the dual staining of neutral complex carbohydrates

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines a concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB) method with a period acid-m-aminophenol-Fast Black salt K (PA-AP-FBK) sequence. With the combined method it is possible to stain α-D-glycosyl and α-D-mannosyl residues brown and 1,2-glycol groups of neutral complex carbohydrates blackish purple. The validity of the method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Avrameas, S. (1970). Emploi de la concanavaline A pour l'isolement, la detection et la mesure des glycoproteines et glucides extra ou endocellulaires.C. r. Acad. Sci. (Paris),270, 2205–12.

    Google Scholar 

  • Casselman, W. G. B. (1959).Histochemical Technique. 1st Edn. p. 100. London: Methuen.

    Google Scholar 

  • Katsuyama, T., Poon, K. C. & Spicer, S. S. (1976). Atypical reactivity of gastric mucous neck cells with variants of the concanavalin A (Con A) + horseradish peroxidase (HRP) method.Proceedings of the Fifth International Congress of Histochemistry and Cytochemistry, Bucharest, Romania, pp. 179–180.

  • Katsuyama, T. & Spicer, S. S. (1977). Paradoxical concanavalin A staining. Its staining mechanism.Proceedings of the 18th Annual Meeting of the Japanese Society of Histochemistry and Cytochemisty., Osaka, Japan, p. 86.

  • Kiernan, J. A. (1975). Localization of α-D-glucosyl and α-D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase.Histochemistry 44, 39–45.

    PubMed  Google Scholar 

  • Lampe, M. &Kiernan, J. A. (1977). Cytochemical variation of mast cells in the skin of the rat.Arch. Derm. Res. 258, 69–80.

    Google Scholar 

  • Leppi, T. J. (1968). Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.Histochemie 15, 68–78.

    PubMed  Google Scholar 

  • Lillie, R. D. (1962). The histochemical reaction of aryl amines with tissue aldehydes produced by periodic and chromic acids.J. Histochem. Cytochem. 10, 303–14.

    Google Scholar 

  • Lillie, R. D. &Fullmer, H. M. (1976).Histopathologic Technic and Practical Histochemistry. 4th Edn. pp. 226–229. New York: McGraw-Hill.

    Google Scholar 

  • Lillie, R. D., Gilmer, P. R. Jr. &Welsh, A. A. (1961). Black periodic and black Bauer methods for tissue polysaccharides.Stain Technol. 36, 361–3.

    PubMed  Google Scholar 

  • McManus, J. F. A. &Mowry, R. W. (1958). Effects of fixation on carbohydrate histochemistry.J. Histochem. Cytochem. 6, 309–16.

    PubMed  Google Scholar 

  • Pearse, A. G. E. (1968).Histochemistry, Theoretical and Applied, Vol. 1. 3rd Edn. pp. 294–380. London: Churchill.

    Google Scholar 

  • Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967). Histochemistry of connective tissue mucopolysaccharides. In:The Connective Tissue (eds. B. M. Wagner & D. E. Smith) pp. 251–303. Baltimore: Williams & Wilkins.

    Google Scholar 

  • Stoddart, R. W. &Kiernan, J. A. (1973). Histochemical detection of the α-D-arabinopyranoside configuration, using fluorescent-labelled concanavalin A.Histochemistry 33, 87–94.

    PubMed  Google Scholar 

  • Weir E. E., Pretlow, T. G., Pitts, A. &Williams, E. E. (1974). Destruction of endogenous peroxidase activity in order to locate antigens by peroxidase-labelled antibodies.J. Histochem. Cytochem. 22, 51–4.

    PubMed  Google Scholar 

  • Yamada, K. &Shimizu, S. (1976). Concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB)-Alcian blue (AB). A reliable method for dual staining of complex carbohydrates.Histochemistry 47, 158–69.

    Google Scholar 

  • Yamada, K. &Shimizu, S. (1977). The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labelledRicinus communis agglutinin.Histochemistry 53, 143–56.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Histology, Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

    Kazuyori Yamada

Authors
  1. Kazuyori Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamada, K. Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-Fast Black salt K: a method for the dual staining of neutral complex carbohydrates. Histochem J 10, 573–584 (1978). https://doi.org/10.1007/BF01003138

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01003138

Keywords

Search

Navigation