Synopsis
A new device is described for improving the accuracy of measuring the thickness of cryostat sections by the focusing technique in the light microscope. The necessity of such measurements is demonstrated by the great variation (range 2.55 μm–11.93 μm) in the thickness of serial cross-sections of frozen muscle biopsies from 12 healthy men. The final dehydration of the sections was found to reduce the thickness of fresh sections by 47%. However, dehydration caused the cross-sectional area to be reduced by only 2.8%.
Similar content being viewed by others
References
Bergström, J. (1962). Muscle electrolytes in man.Scand. J. Clin. Lab. Invest 14, Suppl. 68.
Brattgärd, S.-O. (1954). Microscopical determinations of the thickness of histological sections.Jl. R. micros. Soc. 74, 113–22.
Glimstedt, F. &Hakansson, R (1951). Measurement of thickness in various parts of histological sections.Nature 167, 397–8.
Halkjaer-Kristensen, J. &Ingemann-Hansen, T. (1977). Microphotometry of single fibres from the quadriceps muscle in man.Scand. J. Clin. Lab. Invest. 27, 761–8.
Halkjaer-Kristensen, J. & Ingemann-Hansen, T. (1978). Microphotometric determination of glycogen in single fibres of human skeletal muscle.Scand. J. Clin. Lab. Invest. (in press).
Halkjaer-Kristensen, J. & Ingemann-Hansen, T. (1979). Microphotometric analysis of NADH tetrazolium reductase and α-glycerophosphate dehydrogenase in human musculus quadriceps.Histochem. J. (ms. submitted for publication).
Hallén, O. (1956). On the cutting and thickness determination of microtome sections.Acta Anatomica 26, Suppl. 25.
Ingemann-Hansen T. & Halkjaer-Kristensen, J. (1977). Glycogen content in single fibres of resting human skeletal muscle: a novel approach.Proceedings of the VI International Congress of Biomechanics, Copenhagen, 1977.
Lange, P. W. &Engström, A. (1954). Determination of thickness of microscopic objects.Lab. Invest. 3, 116–31.
Novikoff, A. B., Shin, W.-Y. &Drucker, J. (1961). Mitochondrial localization of oxidative enzymes: staining results with two tetrazolium salts.J. Biophys. Biochem. Cytol. 9, 47–61.
Padykula, H. A. &Herman, E. (1955). The specificity of the histochemical method for adenosine triphosphatase.J. Histochem. Cytochem. 3, 170–95.
Pearse, A. D. &Marks, R. (1974). Measurement of section thickness in quantitative microscopy with special reference to enzyme histochemistry.J. Clin. Path. 27, 615–8.
Pearse, A. G. E. (1968).Histochemistry. Theoretical and Applied., Vol. I, 3rd ed. Churchill Livingstone, Edinburgh.
Pearse, A. G. E. (1972).Histochemistry. Theoretical and Applied. Vol. II, 3rd ed. Edinburgh: Churchill Livingstone.
Wattenberg, L. W. &Leong, J. L. (1960). Effects of coenzyme Q10 and menadione on succinic dehydrogenase activity as measured by tetrazolium salt reduction.J. Histochem. Cytochem. 8, 296–303.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Halkjaer-Kristensen, J., Ingemann-Hansen, T. Thickness measurements of skeletal muscle sections using the light microscope. Histochem J 10, 497–504 (1978). https://doi.org/10.1007/BF01003132
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01003132