The Histochemical Journal

, Volume 3, Issue 2, pp 151–161 | Cite as

Subcellular distributions and properties of rat testicular acid phosphatases

  • Tapani Vanha-Perttula
Papers

Synopsis

Acid phosphatase activities againstp-nitrophenyl phosphate, α-naphthyl phosphate β-naphthyl phosphate, naphthol AS-BI phosphate, α-glycerophosphate and β-glycerophosphate were studied in whole homogenates and subcellular fractions of rat testes. The mitochondrial-lysosomal fraction (the 9000 g sediment) was the most active against all these substrates, but a high specific activity was also present in the soluble fraction (105,000 g supernatant). The effect of various inhibitors and activators onp-nitrophenyl phosphate hydrolysis was different in these fractions. The soluble enzyme was markedly stimulated by Co2+, Mg2+, Mn2+, Ni2+ and Zn2+. The particulate enzyme was inhibited by sodium fluoride, sodium tartrate and sodium molybdate, whereas the soluble activity was sensitive to Cd2+, Cu2+ and Sn2+ as well as to formaldehyde and glutaraldehyde. The soluble acid phosphatase is mainly localized within the seminiferous tubules. The high sensitivity of the soluble activity to the commonly used fixatives may interfere in the histochemical demonstration of the enzyme.

Keywords

Acid Phosphatase Naphthol Seminiferous Tubule Acid Phosphatase Activity Sodium Fluoride 

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References

  1. Allison, A. C. &Hartree, E. F. (1970). Lysosomal enzymes in the acrosome and their possible role, in fertilization.J. Reprod. Fert. 22, 501–15.Google Scholar
  2. Bublitz, G., Merker, H. J. &Günther, T. (1968). Uber das Verhalten und die Lokalisation der sauren Phosphatase des Rattenhodens nach Einwirkung ionisierender Strahlen.Fortschr. Röntgenstr. 108, 238–45.Google Scholar
  3. Buongiorno-Nardelli, M. &Nicholson, A. (1967). Studio istochimico della spermioistogenesi di ratto.Riv. istoch. norm. Pat. 12, 333–42.Google Scholar
  4. Dipietro, D. L. &Zengerle, F. S. (1967). Separation and properties of three acid phosphatases from human placenta.J. biol. Chem. 242, 3391–6.Google Scholar
  5. Ericsson, J. L. E. &Trump, B. F. (1964). Electron microscopic studies of the epithelium of the proximal tubule of the rat kidney. I. The intracellular localization of acid phosphatase.Lab. Invest. 13, 1427–56.Google Scholar
  6. Fiske, C. H. &Subbarow, Y. (1925). The colorimetric determination of phosphorus.J. biol. Chem. 66, 375–400.Google Scholar
  7. Frank, A. L. &Christensen, A. K. (1968). Localization of acid phosphatase in lipofuscin granules and possible autophagic vacuoles in interstitial cells of the guinea pig testis.J. Cell Biol. 36, 1–13.Google Scholar
  8. Friend, D. S. (1969). Cytochemical staining of multivesicular body and Golgi vesicles.J. Cell Biol. 41, 269–79.Google Scholar
  9. Heinrikson, R. L. (1969). Purification and characterization of a low molecular weight acid phosphatase from bovine liver.J. biol. Chem. 244, 299–307.Google Scholar
  10. Hogeboom, G. H., Schneider, W. C. &Striebich, M. J. (1952). Cytochemical studies. V. On the isolation and biochemical properties of liver cell nuclei.J. biol. Chem. 196, 111–20.Google Scholar
  11. Lowry, O. H., Rosebrough, N. J., Farr, A. L. &Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. biol. Chem. 193, 265–75.Google Scholar
  12. Lundin, L. G. &Allison, A. C. (1966). Acid phosphatases from different organs and animal forms compared by starch-gel electrophoresis.Acta chem. scand. 20, 2579–92.Google Scholar
  13. Maggi, V. (1969). Lysosomal and non-lysosomal localization of acid hydrolases in animal cells.Biochem. J. 111, 25P-6P.Google Scholar
  14. Moore, B. W. &Angeletti, P. U. (1961). Chromatographic heterogeneity of some enzymes in normal tissues and tumors.Ann. N.Y. Acad. Sci. 94, 659–67.Google Scholar
  15. Neil, M. W. &Horner, M. W. (1964). Studies on acid hydrolases in adult and foetal tissues. Acidp-nitrophenyl phosphate phosphohydrolases of adult guinea-pig liver.Biochem. J. 92, 217–24.Google Scholar
  16. Neil, M. W. &Horner, M. W. (1964). Studies on acid hydrolases in adult and foetal tissues. 2. Acid phenyl phosphomonoesterases of adult liver.Biochem. J. 93, 220–4.Google Scholar
  17. Nelson, D. (1966). Rat liver acid phosphatase: differences in lysosomal and cytoplasmic forms.Proc. Soc exp. Biol. Med. (N.Y.),121, 998–1001.Google Scholar
  18. Niemi, M. &Kormano, M. (1965). Cylical changes in and significance of lipids and acid phosphatase in the seminiferous tubules of the rat testis.Anat. Rec. 151, 159–70.Google Scholar
  19. Novikoff, A. B. (1963). Lysosomes in the physiology and pathology of cells: contributions of staining methods. ‘Ciba Foundation Symposium on Lysosomes’ (eds. A. V. S. de Reuck and M. P. Cameron), pp. 36–77. London: Churchill.Google Scholar
  20. Rosenbaum, R. M. &Rolon, C. I. (1962). Species variability and the substrate specificity of intracellular acid phosphatases: a comparison of the lead-salt and azo-dye methods.Histochemie 3, 1–16.Google Scholar
  21. Ruffilli, A. (1960). La Fosfatasi acida nell'acrosoma degli spermatidi e nel reticolo di Golgi delle cellule dell'epididimo di Cavia.Ric. Scient. 30, 145–7.Google Scholar
  22. Seljelid, R. (1966). An electron microscopic study of the formation of cytosomes in a rat kidney adenoma.J. Ultrastruct. Res. 16, 569–83.Google Scholar
  23. Shibko, S. &Tappel, A. L. (1963). Acid phosphatases of the lysosomal and soluble fractions of rat liver.Biochim. Biophys. Acta 73, 76–86.Google Scholar
  24. Smith, J. K. &Whitby, L. G. (1968). The heterogeneity of prostatic acid phosphatase.Biochim. Biophys. Acta 151, 607–18.Google Scholar
  25. Tice, L. W. &Barrnett, R. J. (1963). The fine structural localization of some testicular phosphatases.Anat. Rec. 147, 43–63.Google Scholar

Copyright information

© Chapman and Hall Ltd 1971

Authors and Affiliations

  • Tapani Vanha-Perttula
    • 1
  1. 1.Department of AnatomyUniversity of TurkuFinland

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