Advertisement

The Histochemical Journal

, Volume 3, Issue 2, pp 101–106 | Cite as

Carbohydrate-rich compounds in the colonic mucosa of man. III. A preliminary report of some biochemical characteristics of the carbohydrate-rich components in normal colonic mucosa

  • L. Kalevi Korhonen
  • Veikko Mäkelä
Papers

Synopsis

Preparations of human colonic epithelial cells were obtained virtually free from contamination by connective tissue. A trichloroacetic acid-soluble carbohydrate-rich fraction was prepared after defatting, proteolytic digestion and trichloroacetic acid precipitation of proteins by precipitating the polysaccharides with ethanol and potassium acetate. Four components of acid mucosubstances were revealed on electrophoresis, three of them showing the presence of sulphate radicals. The periodate consumption rate of the preparation was over four times greater after methanolytic desulphation, and confirms previous results concerning the blocking of periodate reactivity by acid radicals. The analysis of the sample showed the presence of 15% protein, 14% hexose, 9% sialic acid, 6% hexosamine, 3% fucose and 3% uronic acid.

Keywords

Polysaccharide Sialic Acid Trichloroacetic Acid Hexose Periodate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bitter, T. &Muir, H. M. (1962). A modified uronic acid carbazole reaction.Analyt. Biochem. 4, 330–42.Google Scholar
  2. Dische, Z. &Shettles, L. B. (1951). A new spectrophotometric test for the detection of methylpentose.J. biol. Chem. 192, 579–82.Google Scholar
  3. Dittlebrandt, M. (1948). Application of the Weichselbaum biuret reagent to the determination of spinal fluid protein.Am. J. clin. Path. 18, 439–41.Google Scholar
  4. Glass, G. B. J. (1962). Biologically active material related to gastric mucus in the normal and in the diseased stomach of man.Gastroenterology 43, 310–25.Google Scholar
  5. Gottschalk, A. (1960).The Chemistry and Biology of Sialic Acids and Related Substances. London & New York: Cambridge University Press.Google Scholar
  6. Hieronymi, G. (1954). Über Vorkommen und Verteilung saurer Mucopolysacchariden in Geschwulsten.Frank. Z. Path. 65, 409–34.Google Scholar
  7. Häkkinen, I., Korhonen, L. K. &Saxen, L. (1968). The time of appearance and distribution of sulphoglycoprotein antigens in the human foetal alimentary canal.Int. J. Cancer 3, 582–92.Google Scholar
  8. Kabat, E. A. &Mayer, M. M. (1967).Experimental Immunochemistry, 2nd Ed. Springfield, Ill., U.S.A.: Thomas.Google Scholar
  9. Kantor, T. G. &Schubert, M. (1957). A method for the desulphation of chondroitin sulphate.J. Amer. chem. Soc. 79, 152–3.Google Scholar
  10. Korhonen, L. K. &Mäkelä, V. (1968). Carbohydrate-rich tissue components in lung cancer and in normal tissues: a histochemical study.Histochem. J. 1, 124–40.Google Scholar
  11. Lillie, R. D. (1965).Histopathologic Technique and Practical Histochemistry, 3rd Ed. New York & London: McGraw Hill.Google Scholar
  12. Long, C. (1961).Biochemists' Handbook. London: Spon.Google Scholar
  13. Meyer, K., Smyth, E. M. &Palmer, J. W. (1937). On glycoproteins. III. The polysaccharides from pig gastric mucosa.J. biol. Chem. 119, 73–83.Google Scholar
  14. Mäkelä, V., Korhonen, L. K. & Lilius, G. (1970). Carbohydrate-rich compounds in the colonic mucosa of man. I. Histochemical characteristics of normal and adenomatous colonic mucosa.Cancer, in press.Google Scholar
  15. Odin, L. (1958). Mucopolysaccharides of epithelial origin. InChemistry and Biology of Mucopolysaccharides (eds. G. E. W. Wolstenholme and M. O'Connor). Ciba Symposium, pp. 234–44. London: Churchill.Google Scholar
  16. Schrager, J. (1963). Chromatographic observations of human gastric mucopolysaccharides.Nature, Lond. 198, 899–900.Google Scholar
  17. Scott, J. E. &Harbinson, R. J. (1968). Periodate-oxidation of acid polysaccharides. Inhibition by the electrostatic field of the substrate.Histochemie 14, 215–20.Google Scholar
  18. Seibert, F. S., Seiber, M. V., Alno, A. J. &Cambell, H. W. (1947). Variation in protein and polysaccharide content of sera in the chronic diseases, tuberculosis, sarcoidosis and carcinoma.J. clin. Invest. 26, 90–102.Google Scholar
  19. Warren, L. (1959). The thiobarbituric acid assay of sialic acid.J. biol. Chem. 234, 1971–5.Google Scholar
  20. Winzler, R. J. (1955). Determination of serum glycoproteins. InMethods of Biochemical Analysis (ed. D. Glick), Vol. 11, pp. 279–312. New York: Interscience.Google Scholar
  21. Winzler, R. J. (1965). The amino sugars.Acad. Press 2, 337.Google Scholar

Copyright information

© Chapman and Hall Ltd 1971

Authors and Affiliations

  • L. Kalevi Korhonen
    • 1
    • 2
  • Veikko Mäkelä
    • 1
    • 2
  1. 1.Department of AnatomyUniversity of TurkuFinland
  2. 2.II Department of PathologyUniversity of HelsinkiFinland

Personalised recommendations