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Oxidative enzymes and myosin-ATPase in the trunk musculature of the river lamprey (Lampetra fluviatilis)

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The distribution and activities of several oxidative enzymes and of myosin-ATPase have been studied in the trunk musculature of the river lamprey. The strongest activity of oxidative enzymes and of myosin-ATPase was shown by the parietal fibre bundles of the myotomal subunits, whilst the central fibres reacted to a lesser extent.

Pre-incubation of sections in media containing formaldehyde and glycine or with buffers of different pH, followed by incubation for myosin-ATPase, also differentiated the muscle fibres into a specific distribution pattern within the myotomes, resembling that of the oxidative enzymes to some extent.

The parietal fibres were found to be of the slow type (type I) and the central fibres to be fast (type II A).

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References

  • Andersen, P. &Henriksson, J. (1977). Training induced changes in the subgroups of human type II skeletal muscle fibres.Acta physiol. scand. 99, 123–5.

    Google Scholar 

  • Bone, Q. (1964). Patterns of muscular innervation in lower chordates.Int. Rev. Neurobiol. 6, 99–147.

    Google Scholar 

  • Bozner, A. &Knieriem, H. J. (1970). Beitrag zur Ultrastruktur der Herz- und Skelettmuskulatur des Flußneunauges (Lampetra fluviatilis).Biologia (Bratisl.) 25, 159–68.

    Google Scholar 

  • Brooke, M. H. &Kaiser, K. K. (1970). Muscle fiber types: How many and what kind.Arch. Neurol. 23, 369–79.

    Google Scholar 

  • Burke, R. E. &Tsairis, P. (1974). The correlation of physiological properties with histochemical characteristics in single muscle units.Ann. N.Y. Acad. Sci. 228, 145–59.

    Google Scholar 

  • Eurke, R. E., Levine, D. N., Zajac, F. E., Tsairis, P., &Engel, W. K. (1971). Mammalian motor units: physiological-histochemical correlation in three types in cat gastrocnemius.Science 174, 709–12.

    Google Scholar 

  • Burstone, M. S. (1960). Histochemical demonstration of cytochrome oxidase with new amine reagents.Histochem. Cytochem. 8, 63–70.

    Google Scholar 

  • Dahl, H. A. &Nicolaysen, K. (1971). Actomyosin ATPase activity in Atlantic hagfish muscles.Histochemie 28, 205–10.

    Google Scholar 

  • Fominykh, M. Y. (1970). Morphological differentiation of nerve muscle units in the lampreyLampetra fluviatilis.Zh. Evol. Biokhim. Fiziol. 6, 432–36. (in Russian).

    Google Scholar 

  • Gerebtzoff, M. A. (1956). Contribution à la morphologie comparée des appareils cholinestérasiques myo-neurale et musculo-tendineu des vertébrés.Ann. Histochim. 1, 145–59.

    Google Scholar 

  • Geyer, G. (1973).Ultrahistochemie. Histochemische Arbeitsvorschriften für die Elektronenmikroskopie. 2nd Edn, Stuttgart: Fischer.

    Google Scholar 

  • Guth, L. &Samaha, F. J. (1970) Procedure for the histochemical demonstration of actomyosin ATPase.Exp. Neurol. 28, 365–7.

    Google Scholar 

  • Johnston, L. A. &Lucking, M. (1978). Temperature induced variation in the distribution of different types of muscle fibre in the goldfish (Carassius auratus).J. comp. Physiol. B 124, 111–7.

    Google Scholar 

  • Johnston, I. A., Ward, P. S. &Goldspink, G. (1975). Studies on the swimming musculature of the rainbow trout. I. Fibre types.J. Fish Biol. 7, 451–8.

    Google Scholar 

  • Korneliussen, H., Dahl, H. A. &Paulsen, J. E. (1978). Histochemical definition of muscle fibre types in the trunk musculature of a teleost fish.Histochemistry 55, 1–16.

    Google Scholar 

  • Lojda, Z., Gossrau, R. &Schiebler, T. H. (1976).Enzymhistochemische Methoden. Berlin, Heidelberg, New York. Springer.

    Google Scholar 

  • Mellgren, S. I. &Storm-Mathisen, J. S. (1966). Oxidative enzymes, glycogen and lipid in striated muscle. A histochemical study in the Atlantic hagfish (Myxine glutinosa L.)Z. Zellforsch. 71, 169–88.

    Google Scholar 

  • Nachlas, M. M., Tsou, K. C., De Souza, E., Cheng, C., &Seligman, A. M. (1957). Cytological demonstration of succinic dehydrogenase by use of a newp-nitrophenol substituted ditetrazole.J. Histochem. Cytochem. 5, 420–36.

    Google Scholar 

  • Patterson, S., Johnston, I. A. &Goldspink, G. (1975). A histochemical study of the lateral muscles of five teleost species.J. Fish Biol. 7, 159–66.

    Google Scholar 

  • Pearse, A. G. E. (1972).Histochemistry. Theoretical and Applied. 3rd Edn., Vol. 2. Edinburgh, London: Churchill-Livingstone.

    Google Scholar 

  • Peters, A. &Mackay, B. (1961) The structure and innervation of the myotomes of the lamprey.J. Anat. 95, 575–85.

    Google Scholar 

  • Pette, D., Müller, W., Leisner, E. &Vrbová, G. (1976). Time dependent effects on contractile properties, fibre population, myosin light chains and enzymes of energy metabolism in intermittently and continuously stimulated fast twitch muscles of the rabbit.Pflügers Arch. 364, 103–12.

    Google Scholar 

  • Scarpelli, D. G., Hess, R. &Pearse, A. G. E. (1958). The cytochemical localization of oxidative enzymes. I. Diphosphopyridine nucleotide diaphorase.J. biophys. biochem. Cytol. 4, 747–51.

    Google Scholar 

  • Szentkuti, L. &Cassens, R. G. (1978). Die Verteilung der Fasertypen I, II A und II B im M. longissimus dorsi und M. semitendinosus von Schweinen verschiedenen Alters.Dt. Tierärztl. Wschr. 85, 23–7.

    Google Scholar 

  • Teräväinen, H. (1971). Anatomical and physiological studies on muscles of lamprey.J. Neurophysiol. 34, 954–73.

    Google Scholar 

  • Teräväinen, H. &Rovainen, C. M. (1971). Fast and slow motoneurons to body muscle of the sea lamprey.J. Neurophysiol. 34, 990–8.

    Google Scholar 

  • Tunell, G. L. &Hart, M. N. (1977). Simultaneous determination of skeletal muscle fiber, types I, II A, and II B by histochemistry.Arch. Neurol. 34, 171–3.

    Google Scholar 

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Meyer, W. Oxidative enzymes and myosin-ATPase in the trunk musculature of the river lamprey (Lampetra fluviatilis). Histochem J 11, 187–195 (1979). https://doi.org/10.1007/BF01002995

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