Summary
Rabbit antibody to calf adenosine deaminase (ADA) was used to localize this enzyme in tissues of the young rat and calf by the immunoperoxidase method. The distribution patterns of ADA in most tissues were similar for both species. Within the thymus gland, the enzyme was strongly expressed predominantly in cortical lymphocytes. In the spleen and lymph nodes, most lymphocyles of T-cell areas stained weakly for ADA, whereas only a small number of ADA-positive cells were found in B-cell areas. Clumps of strongly ADA-positive mononuclear blastoid and plasma cells were observed in the medullary regions of lymph nodes, around peri-arteriolar lymphocyte sheaths and in the red pulp of the spleen, and in the lamina propria of the intestine. Double immunofluorescence staining studies in the rat showed that some of these blastoid cells contained both ADA and immunoglobulins and appeared to be plasmablasts. Strong staining for ADA was also found, in both the rat and calf, in as yet unidentified mononuclear blastoid cells in the inter-stitium of non-lymphoid organs (kidney, heart, lung), in endothelial cells of some arterioles and capillaries, and in Kupffer cells of the liver. In addition, ADA was strongly expressed in calf bile canaliculi. These studies define areas in rat and calf tissues which contain ADA-positive cells and provide a model system for investigations of the relationship between ADA and the function and development of these cells.
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References
Adams, A. &Harkness, R. A. (1976) Adenosine deaminase activity in thymus and other human tissues.Clin. exp. Immunol. 26, 647–9.
Ballet, J.-J., Insel, R., Merler, E. &Rosen, F. S. (1976) Inhibition of maturation of human precursor lymphocytes by coformycin, an inhibitor of the enzyme adenosine deaminase.J. exp. Med. 143, 1271–6.
Barton, R., Martiniuk, F., Hirschhorn, R. &Goldschneider, I. (1979) The distribution of adenosine deaminase among lymphocyte populations in the rat.J. Immunol. 122, 216–20.
Ben-Bassat, I., Simoni, F., Holtzman, F. &Ramot, B. (1979) Adenosine deminase activity of normal lymphocytes and leukemia cells.Israel J. Med. Sci. 15, 925–7.
Brady, T. G. &O'donovan, C. I. (1965) A study of the tissue distribution of adenosine deaminase in six mammal species.Comp. Biochem. Physiol. 14, 101–20.
Carson, D. A., Kaye, J. &Seegmiller, J. E. (1977) Lymphospecific toxicity in adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency: possible role of nucleoside kinase(s).Proc. natn. Acad. Sci., U.S.A. 74, 5677–81.
Chechik, B. E., Rao, J., Greaves, M. F. &Hoffbrand, A. V. (1980) Human thymus/ leukemia-associated antigen (a low-molecular weight form of adenosine deaminase) and the phenotype of leukemic cells.Leukemia Res. 4, 343–9.
Chechik, B. E., Schrader, W. P. &Minowada, J. (1981) An immunomorphologic study of adenosine deaminase distribution in human thymus tissue, normal lymphocytes, and hematopoietic cell lines.J. Immun. 126, 1003–7.
Chechik, B. E. &Sengupta, S. (1981) Detection of human, rat, and mouse adenosine deaminase by immunochemical and immunomorphologic methods using antiserum to calf enzyme.J. Immun. Meth. 45, 165–76.
Cohen, A., Hirschhorn, R., Horowitz, S. D., Rubinstein, A., Polmar, S. H., Hong, R. &Martin Jr, D. W. (1978) Deoxadenosine triphosphate as a potentially toxic metabolite in adenosine deaminase deficiency.Proc. natn. Acad. Sci., U.S.A. 75, 472–6.
Coleman, M. S., Greenwood, M. F., Hutton, J. J., Holland, Ph., Lampkin, B., Krill, C. &Kastelic, J. E. (1978) Adenosine deaminase, terminal deoxynucleotidyl transferase (TdT) and cell surface markers in childhood acute leukemia.Blood 52, 1125–31.
Fischer, D., Van der Weyden, M. B., Snyderman, R. &Kelley, W. N. (1976) A role for adenosine deaminase in human monocyte maturation.J. clin. Invest. 58, 399–407.
Fox, I. H. &Kelley, W. N. (1978) The role of adenosine and 2′-deoxyadenosine in mammalian cells.Ann. Rev. Biochem. 47, 655–86.
Gelfand, E. W., Lee, J. J. &Dosch, H. M. (1979) Selective toxicity of purine deoxynucleosides for human lymphocyte growth and function.Proc. natn. Acad. Sci., U.S.A. 76, 1998–2002.
Giblett, E. R., Anderson, J. E., Cohen, F., Pollara, B. &Meuwissen, H. J. (1972) Adenosine deaminase deficiency in two patients with severely impaired cellular immunity.Lancet 2, 1067–9.
Green, H. &Chan, T.-S. (1973) Pyrimidine starvation induced by adenosine in fibroblasts and lymphoid cells: Role of adenosine deaminase.Science 182, 836–7.
Hall, J. G. (1963) Adenosine deaminase activity in lymphoid cells during antibody production.Aust. J. exp. Biol. 41, 93–8.
Hirschhorn, R. &Levitska, V. (1974) Alterations in isozymes of adenosine deaminase during stimulation of human peripheral blood lymphocytes.Cell Immunol. 12, 387–95.
Hirschhorn, R., Martiniuk, F. &Rosen, F. S. (1978) Adenosine deaminase activity in normal tissues and tissues from a child with severe combined immunodeficiency and adenosine deaminase deficiency.Clin. Immun. Immunopath. 9, 287–92.
Huang, A. T., Logue, G. L. &Engelbrecht, H. L. (1976) Two biochemical markers in lymphocyte subpopulations.Br. J. Haemat. 34, 631–8.
Immondi, A. R., Balis, M. E. &Lipkin, M. (1969) Changes in enzyme levels accompanying differentiation of intestinal epithelial cells.Expl Cell Res. 58, 323–30.
Macdermott, R. P., Tritsch, G. L. &Formeister, J. F. (1980) Adenosine deaminase and nucleoside phosphorylase activity in normal human blood mononuclear cell subpopulations.Clin. exp. Immun. 42, 303–7.
Mayrhofer, G., Bazin, H. &Gowans, J. L. (1976) Nature of cells binding anti-IgE in rats immunized withNippostrongylus brasiliensis: IgE synthesis in regional nodes and concentration in mucosal mast cells.Eur. J. Immun. 6, 537–45.
Meuwissen, H. J., Pollara, B. &Pickering, R. J. (1975) Combined immunodeficiency disease associated with adenosine deaminase deficiency.J. Pediat. 86, 169–81.
Okamura, S., Chechik, B. E., Lee, G., Gelfand, E. W. &Mak, T. W. (1981) Heterogeneity of human thymocytes and malignant T-lymphoblasts.Cancer Res. 41, 1664–8.
Polmar, S. H., Stern, R. C., Schwartz, A. L., Wetzler, E. M., Chase, P. A. &Hirschhorn, R. (1976) Enzyme replacement therapy for adenosine deaminase deficiency and severe combined immunodeficiency.New Engl. J. Med. 295, 1337–43.
Smyth, J. F., Poplack, D. G., Holiman, B. J., Leventhal, B. G. &Yarbro, G. (1978) Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia.J. clin. Invest. 62, 710–2.
Sullivan, J. L., Osborne, W. R. A. &Wedgwood, R. J. (1977) Adenosine deaminase activity in lymphocytes.J. Haemat. 37, 157–8.
Trotta, P. P. &Balis, M. E. (1977) Structural and kinetic alterations in adenosine deaminase associated with the differentiation of rat intestinal cells.Cancer Res. 37, 2297–305.
Tung, R., Silber, R., Quagliata, F., Conklyn, M., Gottesman, J. &Hirschhorn, R. (1976) Adenosine deaminase activity in chronic lymphocytic leukemia.J. clin. Invest. 57, 756–61.
Van der Weyden, M. B. &Kelley, W. N. (1976) Human adenosine deaminase. Distribution and properties.J. biol. Chem. 251, 5448–56.
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Chechik, B.E., Baumal, R. & Sengupta, S. Localization and identity of adenosine deaminase-positive cells in tissues of the young rat and calf. Histochem J 15, 373–387 (1983). https://doi.org/10.1007/BF01002970
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DOI: https://doi.org/10.1007/BF01002970