Skip to main content
SpringerLink
Log in

Atrial natriuretic peptide in the ventricles of patients with dilated cardiomyopathy and human foetuses

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

Immunohistochemical examination of atrial natriuretic peptide (ANP) was performed on endomyocardial biopsy specimens from 8 patients with dilated cardiomyopathy (DCM), 10 human foetal hearts obtained from legal abortions, and 8 adult hearts from autopsy control subjects without cardiovascular diseases. The indirect immunoperoxidase method, using specific monoclonal antibody to α-human ANP was employed. Immunoreactivity was observed at the light microscope level in the working ventricular cardiocytes of all patients with DCM as dark-brown, granular deposits. Peripheral plasma levels of ANP in these patients were also increased. In control adult hearts without cardiovascular diseases, immunoreactivity was detected both in the atria and in the ventricular impulse-conducting system, although the working ventricular cardiocytes were not immunoreactive. In foetal hearts, immunoreactivity was detected not only in the atria and ventricular impulse-conducting system, but also in the working ventricular cardiocytes. We conclude that ANP is present in the ventricular impulse-conducting system of the human hearts, and that ANP is also present in the working ventricular cardiocytes in patients with DCM as well as in human foetuses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Back, H., Stumpf, W. E., Ando, E., Nokihara, K. &Forssmann, W. G. (1986) Immunocytochemical evidence for CDD/ANP-like peptide in strands of myoendocrine cells associated with the ventricular conduction system of the rat heart.Anat. Embryol. 175, 223–6.

    Google Scholar 

  • Ballermann, B. J. &Brenner, B. M. (1986) Role of atrial peptides in body fluid homeostasis.Circ. Res. 58, 619–30.

    Google Scholar 

  • Bloch, K. D., Seidman, J. G., Naftilan, J. D., Fallon, J. T. &Seidman, C. E. (1986) Neonatal atria and ventricles secrete atrial natriuretic factor via tissue-specific secretory pathways.Cell 47, 695–702.

    Google Scholar 

  • Burnett, J. C. Jr, Kao, P. C., Hu, D. C., Heser, D. W., Heublein, D., Granger, J. P., Opgenorth, T. J. &Reeder, G. S. (1986) Atrial natriuretic peptide elevation in congestive heart failure in the human.Science 231, 1145–7.

    Google Scholar 

  • Cantin, M., Gutkowska, J., Thibault, G., Milne, R. W., Ledoux, S., Minli, S., Chapeau, C., Garcia, R., Hamet, P. &Genest, J. (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands.Histochemistry 80, 113–27.

    Google Scholar 

  • Cheung, C. Y., Gibbs, D. M. &Brace, R. A. (1987) Atrial natriuretic factor in maternal and fetal sheep.Am. J. Physiol. 252, E279–82.

    Google Scholar 

  • De Bold, A. J. (1985) Atrial natriuretic factor: a hormone produced by the heart.Science 230, 767–70.

    Google Scholar 

  • Edwards, B. S., Ackermann, D. M., Lee, M. E., Reeder, G. S., Wold, L. E. &Burnett, J. C. (1988) Identification of atrial natriuretic factor within ventricular tissue in hamsters and humans with congestive heart failure.J. Clin. Invest. 81, 82–6.

    Google Scholar 

  • Gardner, D. G., Deschepper, C. F., Ganong, W. F., Hane, S., Fiddes, J., Baxter, J. D. &Lewicki, J. (1986) Extra-atrial expression of the gene for atrial natriuretic factor.Proc. Natl. Acad. Sci. USA 83, 6697–701.

    Google Scholar 

  • Izumo, S., Nadal-Ginard, B. &Mahdavi, V. (1988) Protooncogene induction and reprogramming of cardiac gene expression produced by pressure overload.Proc. Natl. Acad. Sci. USA 85, 339–43.

    Google Scholar 

  • Jamieson, J. D. &Palade, G. E. (1964) Specific granules in atrial muscle cells.J. Cell Biol. 23, 151–72.

    Google Scholar 

  • Kangawa, K. &Matsuo, H. (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP).Biochem. Biophys. Res. Commun. 118, 131–9.

    Google Scholar 

  • Kikuchi, K., Nakao, K., Hayashi, K., Morii, N., Sugawara, A., Sakamoto, M., Imura, H. &Mikawa, H. (1987) Ontogeny of atrial natriuretic polypeptide in the human heart.Acta Endocrinol. 115, 211–17.

    Google Scholar 

  • Mukoyama, M., Nakao, K., Sugawa, H., Morii, N., Sugawara, A., Yamada, T., Itoh, H., Shiono, S., Saito, Y., Arai, H., Mori, T., Yamada, H., Sano, Y. &Imura, H. (1988) A monoclonal antibody to α-human atrial natriuretic polypeptide.Hypertension 12, 117–21.

    Google Scholar 

  • Nakao, K., Sugawara, A., Morii, N., Sakamoto, M., Suda, M., Soneda, J., Ban, T., Kihara, M., Yamori, Y., Shimokura, M., Kiso, Y. &Imura, H. (1984) Radioimmunoassay for α-human and rat atrial natriuretic polypeptide.Biochem. Biophys. Res. Commun. 124, 815–21.

    Google Scholar 

  • Nakao, K., Sugawara, A., Shiono, S., Saito, Y., Morii, N., Yamada, T., Itoh, H., Mukoyama, M., Arai, H., Sakamoto, M. &Imura, H. (1987) Secretory form of atrial natriuretic polypeptide as cardiac hormone in humans and rats.Can. J. Physiol. Pharmacol. 65, 1756–61.

    Google Scholar 

  • Nakayama, K., Ohkubo, H., Hirose, T., Inayama, S. &Nakanishi, S. (1984) mRNA sequence for human cardiodilatin-atrial natriuretic factor precursor and regulation of precursor mRNA in rat atria.Nature 310, 699–701.

    Google Scholar 

  • Needleman, P. &Greenwald, J. E. (1986) Atriopeptin: a cardiac hormone intimately involved in fluid, electrolyte, and blood-pressure homeostasis.N. Engl. J. Med. 314, 828–34.

    Google Scholar 

  • Saito, Y., Nakao, K., Nishimura, K., Sugawara, A., Okumura, K., Obata, K., Sonoda, R., Ban, T., Yasue, H. &Imura, H. (1987a) Clinical application of atrial natriuretic polypeptide in patients with congestive heart failure: beneficial effects on left ventricular function.Circulation 76, 115–24.

    Google Scholar 

  • Saito, Y., Nakao, K., Arai, H., Sugawara, A., Morii, N., Yamada, T., Itoh, H., Shiono, S., Mukoyama, M., Obata, K., Yasue, H., Ohkubo, H., Nakanishi, S. &Imura, H. (1987b) Atrial natriuretic polypeptide (ANP) in human ventricle. Increased gene expression of ANP in dilated cardiomyopathy.Biochem. Biophys. Res. Commun. 148, 211–17.

    Google Scholar 

  • Scott, J. N. &Jennes, L. (1987) Distribution of atrial natriuretic factor in fetal rat atria and ventricles.Cell Tissue Res. 248, 479–81.

    Google Scholar 

  • Sugawara, A., Nakao, K., Morri, N., Sakamoto, M., Suda, M., Shimokura, M., Kiso, Y., Kihara, M., Yamori, Y., Nishimura, K., Soneda, J., Ban, T. &Imura, H. (1985) α-Human atrial natriuretic polypeptide is released from the heart and circulates in the body.Biochem Biophys. Res. Commun. 129, 439–46.

    Google Scholar 

  • Thompson, R. P., Simon, J. A. V. &Currie, M. G. (1986) Atriopeptin distribution in the developing rat heart.Anat. Embryol. 175, 227–33.

    Google Scholar 

  • Tikkanen, I., Fyhrquist, F., Metsarinne, K. &Leidenius, R. (1985) Plasma atrial natriuretic peptide in cardiac disease and during infusion in healthy volunteers.Lancet 2, 66–9.

    Google Scholar 

  • Toshimori, H., Toshimori, K., Oura, C. &Matsuo, H. (1987) Immunohistochemistry and immunocytochemistry of atrial natriuretic polypeptide in porcine heart.Histochemistry 86, 595–601.

    Google Scholar 

  • Tsuchimochi, H., Yazaki, Y., Ohno, H., Takanashi, R. &Takaku, F. (1987) Ventricular expression of atrial natriuretic peptide.Lancet 2, 336–7.

    Google Scholar 

  • Wei, Y., Rodi, C. P., Day, M. L., Wiegand, R. C., Needleman, L. D., Cole, B. R. &Needleman, P. (1987) Developmental changes in the rat atriopeptin hormonal system.J. Clin. Invest. 79, 1325–9.

    Google Scholar 

  • Wharton, J., Anderson, R. H., Springall, D., Power, R. F., Rose, M., Smith, A., Espejo, R., Khanghani, A., Wallwork, J., Yacoub, M. H. &Polak, J. M. (1988) Localization of atrial natriuretic peptide immunoreactivity in the ventricular myocardium and conduction system of the human fetal and adult heart.Br. Heart J. 60, 267–74.

    Google Scholar 

  • Yamaji, T., Hirai, N., Ishibashi, M., Takaku, F., Yanaihara, T. &Nakayama, T. (1986) Atrial natriuretic peptide in umbilical cord blood: Evidence for a circulating hormone in human fetus.J. Clin. Endocrinol. Metab. 63, 1414–17.

    Google Scholar 

  • Yasue, H., Obata, K., Okumura, K., Kurose, M., Ogawa, H., Matsuyama, K., Jougasaki, M., Saito, Y., Nakao, K. &Imura, H. (1989) Increased secretion of atrial natriuretic polypeptide from the left ventricle in patients with dilated cardiomyopathy.J. Clin. Invest. 83, 46–51.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Cardiology, Kumamoto University Medical School, 1-1-1 Honjo, 860, Kumamoto, Japan

    Michihisa Jougasaki, Hirofumi Yasue & Ken Okumura

  2. Second Division, Department of Medicine, Kyoto University School of Medicine, 606, Kyoto, Japan

    Masashi Mukoyama, Yoshihiko Saito & Kazuwa Nakao

  3. Second Department of Pathology, Kumamoto University Medical School, 860, Kumamoto, Japan

    Kiyoshi Takahashi

Authors
  1. Michihisa Jougasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hirofumi Yasue
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken Okumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masashi Mukoyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshihiko Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kazuwa Nakao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kiyoshi Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jougasaki, M., Yasue, H., Okumura, K. et al. Atrial natriuretic peptide in the ventricles of patients with dilated cardiomyopathy and human foetuses. Histochem J 21, 715–720 (1989). https://doi.org/10.1007/BF01002837

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002837

Keywords

Search

Navigation