The Histochemical Journal

, Volume 11, Issue 4, pp 473–483 | Cite as

Differentiation of the formaldehyde-induced fluorescent products of noradrenaline and dopamine by microspectrofluorometry

  • N. G. M. Wreford
  • G. C. Smith
Papers

Synopsis

The influence of concentration of dopamine and noradrenaline on the spectral characteristics of their formaldehyde-induced fluorophore, together with the influence of duration of reaction with formaldehyde, has been investigated in a model system. No substantial differences between fluorescence spectra of the amine fluorophores were observed. Accordingly, the influence of hydrochloric acid and ammonia vapours on the fluorophores was investigated. A shift to shorter wavelengths in the excitation maximum of each fluorophore was observed after a brief exposure to hydrochloric acid vapour; more prolonged exposure resulted in a pronounced shift back to longer wavelengths in the case of noradrenaline but no substantial change was observed with dopamine. Brief exposure to hydrochloric acid vapour resulted in a substantial increase in the rate of fading of the noradrenaline fluorophore on exposure to exciting radiation. It is suggested that this offers a convenient way of differentiating the amines.

Keywords

Ammonia Formaldehyde Dopamine Noradrenaline Hydrochloric Acid 

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Copyright information

© Chapman and Hall Ltd. 1979

Authors and Affiliations

  • N. G. M. Wreford
    • 1
  • G. C. Smith
    • 1
  1. 1.Department of AnatomyMonash UniversityClaytonAustralia

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