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The Histochemical Journal

, Volume 11, Issue 4, pp 457–471 | Cite as

The use of peroxidase-labelledLimulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy

  • K. Yamada
  • S. Shimizu
Papers

Synopsis

The usefulness of a lectin,Limulus polyphemus agglutinin (LPA) has been tested in a series of mammalian tissues with sialic acid-containing glycoproteins. In nearly all the tissues employed, the positive peroxidase-labelled LPA diaminobenzidine (LPA-PO-DAB) reaction of various histological structures was markedly diminished in intensity or abolished, following digestion with neuraminidase. In the same tissues, sialic acid added with LPA-PO abolished the LPA-PO-DAB reaction or notably suppressed its intensity. In the majority of the tissues tested, the LPA-PO-DAB-Alcian Blue (AB) (pH 1.0 or 2.5) procedures appear to be useful dual staining methods which enable one to colour selectively sialic acid-containing and other acidic carbohydrates. In view of the endogenous peroxidase activity in particular histological structures, however, appropriate control staining procedures should be performed when the LPA-PO-DAB procedure is employed, either alone or in combination with AB procedures, to determine the histochemical properties of sialic acid-containing glycoproteins.

Keywords

Carbohydrate Light Microscopy Peroxidase Activity Sialic Acid Diaminobenzidine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1979

Authors and Affiliations

  • K. Yamada
    • 1
  • S. Shimizu
    • 2
  1. 1.Laboratory of Histology, Department of AnatomyNagoya University School of MedicineNagoyaJapan
  2. 2.Laboratory of Ultrastructure ResearchAichi Cancer Center Research InstituteNagoyaJapan

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