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An enzyme histochemical study of isoproterenol-induced myocardial necroses in rats

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Summary

The effect of isoproterenol on myocardial metabolism in rats was studied using qualitative and quantitative histochemical techniques. The activity and location of 20 enzymes that play a role in the aerobic and anaerobic pathways of energy metabolism were qualitatively examined. The activity and location of some hydrolytic enzymes and the glycogen content were also qualitatively studied. For the quantitative study the activity of 10 enzymes was measured.

The isoproterenol injections induced necrosis with inflammatory infiltrates. The muscle fibres in the necrotic regions were characterized by defective aerobic energy metabolism and increased glycolytic capacity. There was a depletion of the glycogen reserves in the necrotic fibres. These fibres showed a markedly increased activity of enzymes belonging to the oxidative branch of the pentose phosphate pathway. The implication of this increase for the metabolism of the myocardial cells is discussed. The activity of acid phosphatase in the pathological muscle fibres was strongly increased. The inflammatory cells in the necrotic areas were characterized by preponderantly anaerobic metabolism.

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Authors and Affiliations

  1. Laboratory of Experimental Neurology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands

    A. E. F. H. Meijer

  2. Department for Theoretical Medicine of the University, Osnabrück, FRG

    H. Hettwer

  3. Gerhard-Domagk Institute for Pathology, Wilhelms University, Münster, FRG

    A. G. Ciplea

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  1. A. E. F. H. Meijer
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  2. H. Hettwer
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Dedicated to Prof. H. G. Goslar in honour of his 70th birthday.

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Meijer, A.E.F.H., Hettwer, H. & Ciplea, A.G. An enzyme histochemical study of isoproterenol-induced myocardial necroses in rats. Histochem J 20, 697–707 (1988). https://doi.org/10.1007/BF01002750

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