Summary
The effect of microwave-generated energy for the demonstration of immunoglobulins in periodate lysine paraformaldehyde dichromate fixed paraffin sections of human reactive tonsil using the immunogold silver staining technique has been evaluated. Results of the study show that microwave stimulation permits incubation times of both the primary antibody and the immunogold reagent to be greatly reduced. Specific staining of equal intensity to that produced using the longer standard procedure together with minimal background staining is achieved.
Similar content being viewed by others
References
Chiu, K. Y. (1987) Use of microwaves for rapid immunoperoxidase staining of paraffin sections.Med. Lab. Sci. 44, 3–5.
Faulk, W. P. &Taylor, G. M. (1971) An immunogold method for the electron microscope.Immunochemistry 8, 1081–3.
Holgate, C. S., Jackson, P., Cowen, P. N. &Bird, C. C. (1983) Immunogold silver staining: new method of immunostaining with enhanced sensitivity.Histochem. Cytochem. 31, 983–44.
Holgate, C. S., Jackson, P., Pollard, K., Lunny, D. &Bird, C. C. (1986) Effect fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin processed tissue.J. Pathol. 149, 293–300.
Leong, A. S. Y., Daymon, M. E. &Milios, J. (1985) Microwave irradiation as a form of fixation for light and electron microscopy.J. Pathol. 146, 313–21.
Mclean, I. W., &Nakane, P. K. (1974) Periodate lysine paraformaldehyde fixative. A new fixative for immunoelectron microscopy.Histochem. Cytochem. 22, 1077–83.
Roth, J. (1983b) Application of lectin gold complexes for electron microscopic localization of glycoconjugates on thin sections.J. Histochem. Cytochem. 31, 987–99.
Zondervan, P. E., Van Der Kwast, Th, De Jong, A., Visser, W. J. &De Bruijn, W. C. (1986)Urol. Res. 14, 331–5.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jackson, P., Lalani, E.N. & Boutsen, J. Microwave-stimulated immunogold silver staining. Histochem J 20, 353–358 (1988). https://doi.org/10.1007/BF01002729
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01002729