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Comparison between biochemical and histochemical assessments of peroxidase activity in rat mammary tumours

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Summary

The endogenous peroxidase content of 26 ‘hormone-dependent’ and 16 ‘hormone-independent’ rat mammary tumours was assessed by means of a biochemical (guaiacol) assay on tumour extracts and by means of a histochemical (diaminobenzidine) technique on frozen sections of the same tumours. By guaiacol assay the hormone-dependent tumours had significantly higher peroxidase levels than the hormone-independent tumours. In contrast, by diaminobenzidine staining of the same tumours, peroxidase was detectable in 94% of hormone-independent tumours but in only 54% of hormone-dependent tumours. Moreover, there was no direct correlation between the results of biochemical and histochemical methods. At least in these rat mammary tumours, therefore, histochemical estimates of peroxidase activity based on the diaminobenzidine reaction do not seem to reflect the same tissue properties as biochemical estimates based on the guaiacol reaction after tissue disruption.

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References

  • Desombre, E. R., Anderson, W. A. &Kang, Y. H. (1975) Identification, subcellular localisation and oestrogen regulation of peroxidase in 7,12 DMBA-induced rat mammary tumours.Cancer Res. 35, 172.

    Google Scholar 

  • Desombre, E. R. &Lyttle, C. R. (1978) Isolation and purification of rat mammary tumour peroxidase.Cancer Res. 38, 86.

    Google Scholar 

  • Duffy, M. J. &Duffy, G. (1977) Peroxidase activity as a possible marker for a functional oestradiol receptor in human breast tumours.Biochem. Soc. Trans. 5, 738.

    Google Scholar 

  • Graham, R. C. &Karnovsky, M. J. (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291.

    Google Scholar 

  • Himmelhoch, S. R., Evans, W. H., Mage, M. G. &Peterson, E. A. (1969) Purification of myeloperoxidases from the bone marrow of the guinea pig.Biochemistry 8, 914.

    Google Scholar 

  • Lyttle, C. R. &Desombre, E. R. (1977) Generality, of oestrogen stimulation of peroxidase activity in growth-responsive tissues.Nature, Lond. 268, 337.

    Google Scholar 

  • Lyttle, C. R., Thorpe, S. M., Desombre, E. R. &Daehnfieldt, J. L. (1979) Peroxidase activity and iodide uptake in hormone-responsive and hormone-independent GR mouse mammary tumour.J. natn. Cancer Inst. 62, 31.

    Google Scholar 

  • Penney, G. C., Scott, K. M. &Hawkins, R. A. (1980) Endogenous peroxidase: an alternative to oestrogen receptor in the management of breast cancer?Br. J. Cancer 41, 648.

    Google Scholar 

  • Scott, A. M., Murphy, S. &Hawkins, R. A. (1979) Synthesis of DNA and Lecithin in tissue culture and oestrogen receptor activity in rat mammary tumours dependent on and independent of the ovary.J. Endocrinol. 81, 183.

    Google Scholar 

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Author notes

  1. G. C. Penney

    Present address: Edinburgh and S.E. Scotland Regional Blood Transfusion Service, Royal Infirmary of Edinburgh, EH3 9YW, Edinburgh, Scotland

Authors and Affiliations

  1. University Department of Clinical Surgery, Royal Infirmary of Edinburgh, Edinburgh, Scotland

    G. C. Penney & R. A. Hawkins

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  1. G. C. Penney
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  2. R. A. Hawkins
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Penney, G.C., Hawkins, R.A. Comparison between biochemical and histochemical assessments of peroxidase activity in rat mammary tumours. Histochem J 13, 983–988 (1981). https://doi.org/10.1007/BF01002638

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  • DOI: https://doi.org/10.1007/BF01002638

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