The Histochemical Journal

, Volume 13, Issue 6, pp 961–973 | Cite as

The immunohistochemical detection of prostatic acid phosphatase: its possibilities and limitations in tumour histochemistry

  • A. C. Jöbsis
  • G. P. De Vries
  • A. E. F. H. Meijer
  • J. S. Ploem
Papers

Summary

The immunological specificity of the Amsterdam rabbit antiserum against human prostatic and phosphatase was studied on paraffin sections of 200 prostatic carcinomas and 330 control tissues using an indirect peroxidase technique. Peripheral blood leucocyte smears were also investigated with a fluorescent technique. In a limited number of cases, the mixed aggregation immunocytochemical method was also applied as post-primary incubation procedure. The diaminobenzidine (DAB) final reaction product of the peroxidase technique, carried out under standard conditions, was quantified in some cases using the Leyden Television Analysis System (LEYTAS) with a built-in standard.

A positive reaction was obtained in 96.5% of the prostatic carcinomas. Only 2.1% of the non-prostatic tumour cases (23 types) showed a positive reaction, namely six out of 10 insulomas and one out of 10 carcinoid tumours. The β-cells of the normal islet of Langerhans and the leucocytes in the smears showed a positive reaction. The sensitivity of the peroxidase method, judged subjectively, is not only dependent on the circumstances of fixation, embedding and incubation but also on the degree of tumour differentiation. None of the three prostatic carcinomas studied reached the level of DAB staining intensity shown by the hyperplastic prostatic epithelium.

Keywords

Acid Phosphatase Positive Reaction Carcinoid Tumour Prostatic Carcinoma Prostatic Acid Phosphatase 

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Copyright information

© Chapman and Hall Ltd 1981

Authors and Affiliations

  • A. C. Jöbsis
    • 1
  • G. P. De Vries
    • 1
  • A. E. F. H. Meijer
    • 1
  • J. S. Ploem
    • 2
  1. 1.Department of PathologyAmsterdam University Hospital ‘Wilhelmina Gasthuis’AmsterdamThe Netherlands
  2. 2.Department of Histochemistry and Cytochemistry, Silvius LaboratoryUniversity of LeidenThe Netherlands

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