Skip to main content
SpringerLink
Log in

The relevance of the histochemistry of colonic mucins based upon their PAS reactivity

  • Review
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Culling, C. F. A. (1974a)Handbook of Histopathological and Histochemical Techniques 3rd edn. Butterworth, London.

    Google Scholar 

  • Culling, C. F. A. (1974b)Modern Microscopy. London: Butterworth.

    Google Scholar 

  • Culling, C. F. A. (1977) PAPS — The unlabelled antibody technique.Bull. Microsc. Soc. Can. 5, 16.

    Google Scholar 

  • Culling, C. F. A. &Reid, P. E. (1977) The apparent failure of sodium borohydride reduction to block further PAS reactivity in rat epithelial mucins.Histochem. J. 9, 781.

    Google Scholar 

  • Culling, C. F. A. &Reid, P. E. (1980). Specific techniques for the identification of O-acylated sialic acids in colonic mucins.J. Microscopy 119, 415.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E., Burton, J. D. &Dunn, W. L. (1975) A histochemical method of differentiating lower gastrointestinal tract mucin from other mucins in primary or metastatic tumours.J. clin. Path. 28, 656.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E., &Dunn, W. L. (1971) The effect of saponification upon certain histochemical reactions of the epithelial mucins of the GI tract.J. Histochem. Cytochem. 19, 654.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1974a) Problems associated with the removal of sialic acids by acid hydrolysis.Stain Technol. 49, 317.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1976) A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids.J. Histochem Cytochem. 24, 1225.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1977b) A histochemical comparison, of the epithelial mucins in the ileum in Crohn's disease and in normal controls.J. clin. Path. 31, 1063.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1979) A histochemical comparison of theO-acylated sialic acids of the epithelial mucins in ulcerative colitis, Crohn's disease and normal controls,J. clin. Path. 32, 1272.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E., Dunn, W. L. &Clay, M. G. (1974b) The histochemical demonstration ofO-acylated sialic acids in gastrointestinal mucins. Their association with the potassium hydroxide-periodic acid-Schiff effect.,J. Histochem. Cytochem. 22, 826.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E., &Sinnott, N. M. (1980) The effect of various fixatives and trypsin digestion upon the staining of routine paraffin embedded, sections by the Peroxidase-Antiperoxidase and Immunofluorescent technique.J. Histotechnol. 3, 10–19.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E., Trueman, L. S., &Dunn, W. L. (1973) A simple method for the isolation of viable epithelial cells of the gastrointestinal tract.Proc. Soc. exp. Biol. Med. 147, 434.

    Google Scholar 

  • Culling, C. F. A., Reid, P. E. & Worth, A. J. (1981) Carbohydrate markers in the lower gastro-intestinal tract.Meth. Achievements exp. Path. in press.

  • Culling, C. F. A., Reid, P. E., Worth, A. J. &Dunn, W. L. (1977a) A new histochemical technique of use in the interpretation and diagnosis of adenocarcinoma and villous lesions in the large intestine.J. clin. Path. 30, 1056–62.

    Google Scholar 

  • Dawson, P. A. &Filipe, M. I. (1975) An ultrastructural application of silver methanamine to the study of mucin changes in the colonic mucosa adjacent to and remote from carcinoma.Histochem. J. 8, 143–58.

    Google Scholar 

  • Dawson, P. A. &Filipe, M. I. (1976) An ultrastructural and histochemical study of the mucous membrane, adjacent to and remote from carcinoma of the colon.Cancer 37, 2388–98.

    Google Scholar 

  • Dawson, P. A., Patel, J. &Filipe, M. I. (1978) Variations in sialomucins in the mucosa of the large intestine in malignancy: a quantimet and statistical analysis.Histochem. J. 10, 559–72.

    Google Scholar 

  • Drzenick, R. (1973) Substrate specificity of neuraminidase.Histochem. J. 9, 781.

    Google Scholar 

  • Fenger, C. &Filipe, M. I. (1977) Pathology of the anal glands with special reference to their mucin histochemistry.Acta path. microbiol. scand. A 85, 273.

    Google Scholar 

  • Filipe, M. I. (1969) Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum.Gut 10, 577–86.

    Google Scholar 

  • Filipe, M. I. (1971) Sulphur uptake in the mucosa adjacent to carcinoma of the large intestine.Histochem. J. 3, 27–35.

    Google Scholar 

  • Filipe, M. I. (1972) The value of a study of the mucosubstances in rectal biopsies from patients with carcinoma of the rectum and lower sigmoid in the diagnosis of premalignant mucosa.J. clin. Path.,25, 123–8.

    Google Scholar 

  • Filipe, M. I. (1975) Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study.Brit. J. Cancer 32, 60–77.

    Google Scholar 

  • Filipe, M. I. (1975) Mucins in the human gastrointestinal epithelium: A review.Invest. Cell Path. 2, 195–216.

    Google Scholar 

  • Filipe, M. I. &Branfoot, A. C. (1974a) Abnormal patterns of mucus secretion in apparantly normal mucosal of large intestine with carcinoma.Cancer 34, 282–90.

    Google Scholar 

  • Filipe, M. I. &Branfoot, A. C. (1976) Mucin histochemistry of the colon. In Morson, B.C. (ed.),Current Topics in Pathology. Springer-Verlag, Heidelberg, pp. 143–78.

    Google Scholar 

  • Filipe, M. I. &Cooke, K. B. (1974b) Changes in the composition of mucins in the mucosa adjacent to carcinoma of the colon as compared with the normal: a biochemical investigation.J. clin. Path. 27, 315–18.

    Google Scholar 

  • Florey, H. W. (1970)General Pathology, 4th edn. p. 195. London: Lloyd Lake.

    Google Scholar 

  • Freeman, H. J. (1980) Experimental animal studies in colonic carcinogenesis and dietary fiber. InMedical Aspects of Dietary Fiber (edited bySpiller, G. A. andKay, R. M.), pp. 83–117. New York: Plenum.

    Google Scholar 

  • Freeman, H. J., Lotan, R. &Kim, Y. S. (1980) Applications of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat.Lab. Invest. 42, 405.

    Google Scholar 

  • Goldman, H. &Ming, S. C. (1968) Mucins in normal and neoplastic gastrointestinal epithelium: histochemical distribution.Arch. Path. 85, 580–86.

    Google Scholar 

  • Gottschalk, A. (1966) Definition of glycoproteins and their delimitization from other carbohydrate-protein complexes. InGlycoproteins: their Composition, Structure and Function. Elsevier, London.

    Google Scholar 

  • Hale, A. J. (1953) Observations on substances that react weakly to the periodic acid schiff test.Quart. J. Micr. Sci. 94, 303.

    Google Scholar 

  • Haverkamp, J., Schauer, R., Wember, M., Kamerling, J. P. &Vliegenthart, J. F. F. (1975) Synthesis of 9-O-acetyl and 4,9-di-O-acetyl neuraminic acid methyl glycoside.Hoppe Seylers Z. Physiol. Chem. 356, 1575.

    Google Scholar 

  • Isaacson, P. &Attwood, P. R. A. (1979) Failure to demonstrate specificity of the morphological and histochemical changes in mucosa adjacent to colonic carcinoma (transitional mucosa).J. clin. Path. 32, 214–18.

    Google Scholar 

  • Morson, B. C. &Dawson, I. M. P. (1972)Gastrointestinal Pathology. Oxford: Blackwell Scientific Publications.

    Google Scholar 

  • Reid, P. E. &Culling, C. F. A. (1980a) The periodate oxidation of carbohydrates in relation to the PAS reaction.J. Histotechnol. 3, 82.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., &Dunn, W. L. (1973) Saponification induced increase in the periodic acid-Schiff reaction in the gastrintestinal tract. Mechanism and distribution of the reactive substance.J. Histochem. Cytochem. 21, 473.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., &Dunn, W. L. (1974) The histochemical interpretation of the complex results of methylation upon G-1 tract mucins with special reference to PAS reactivity.J. Histochem. Cytochem. 22, 986.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A. &Dunn, W. L. (1978a) A histochemical method for the demonstration of 9-O-acyl sialic acids. An investigation of bovine submaxillary gland and intestinal mucins.J. Histochem. Cytochem. 26, 187–92.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., &Dunn, W. L. (1978b) A histochemical method for the identification of 9-O-acyl sialic acids. An investigation of bovine submaxillary gland and intestinal mucin.J. Histochem. Cytochem. 26, 187.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., Dunn, W. L., &Clay, M. G. (1976) The use of a transesterification technique to distinguish between certain neuraminidase resistant epithelial mucins.Histochem.,46, 203.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., Dunn, W. L., Clay, M. G. &Ramey, C. W. (1978b) A correlative chemical and histochemical study of theO-acetylated sialic acids of a human colonic epithelial glucoproteins in formalin fixed paraffin embedded tissues.J. Histochem. Cytochem. 26, 1033.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., Dunn, W. L., Ramey, C. W., Magil, A. B. &Clay, M. G. (1980) Differences between theO-acylated sialic acids of the epithelial mucins of human colonic tumours and normal controls: A correlative chemical and histochemical study.J. Histochem. Cytochem. 28, 217–22.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., Ramey, C. W., Dunn, W. L. &Clay, M. G. (1977) A simple method for the determination of theO-acetyl substitution pattern of the sialic acids of colonic epithelial glycoprotein.Can. J. Biochem. 55, 493.

    Google Scholar 

  • Reid, P. E., Culling, C. F. A., Tsang, Wai-Chiu, Ramey, C. W., Dunn, W. L. &Clay, M. G. (1975) The demonstration ofO-acetylated sialic acids in colonic epithelial glycoproteins.Can. J. Biochem. 53, 388.

    Google Scholar 

  • Rhatigan, R. M. &Saffos, R. O. (1979) Mucosal hyperplasia in colonic diverticula.Histopathol. 3, 153–60.

    Google Scholar 

  • Rogers, C. M., Cooke, K. B. &Filipe, M. I. (1978) The sialic acids of human large bowel mucosa:O-acylated variants in normal and malignant states.Gut 19, 587–92.

    Google Scholar 

  • Scott, J. E. &Dorling, J. (1965) Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions.Histochemie 5, 221–23.

    Google Scholar 

  • Spicer, S. S. (1965) Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–34.

    Google Scholar 

  • Sternberger, L. A. (1974)Immunocytochemistry. New Jersey: Prentice-Hall.

    Google Scholar 

  • Sternberger, L. A. (1977) Immunocytochemistry of neuropeptides and their receptors. InPeptides in Neurobiology, (edited byGainer, H.), New York: Plenum Press.

    Google Scholar 

  • Subbuswamy, S. G. (1971) Mucosubstances in neoplasms of the human colon and rectum.Gut 12, 200.

    Google Scholar 

  • Weedon, D. D., Shorter, R. G. &Ilstrup, D. M. (1973) Crohn's disease and cancer.New Engl. J. Med.,289, 1099.

    Google Scholar 

  • Wood, W. S. &Culling, C. F. A. (1975) Perianal Paget's disease-its histochemical differentiation utilizing the borohydride-KOH-PAS technique.Archs Path. 100, 442.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, University of British Columbia, V6T 1W5, Vancouver, British Columbia, Canada

    C. F. A. Culling, P. E. Reid & W. L. Dunn

  2. Department of Medicine, Faculty of Medicine, University of British Columbia, V6T 1W5, Vancouver, British Columbia, Canada

    H. J. Freeman

Authors
  1. C. F. A. Culling
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. E. Reid
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. L. Dunn
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. J. Freeman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Culling, C.F.A., Reid, P.E., Dunn, W.L. et al. The relevance of the histochemistry of colonic mucins based upon their PAS reactivity. Histochem J 13, 889–903 (1981). https://doi.org/10.1007/BF01002630

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002630

Keywords

Search

Navigation