The Histochemical Journal

, Volume 1, Issue 2, pp 166–175 | Cite as

The relation between nerve fibres and dopamine cells of the ruminant lung

  • Catherine Hebb
  • P. Kasa
  • S. Mann
Papers

Synopsis

A study of the distribution and ultrastructure of the special chromaffin or dopamine cells of the ruminant lung has been made. The results with fluorescence microscopy show that the adult sheep has larger numbers of such cells than has the lamb, calf or adult goat. Contrary to earlier findings, their numbers have been found to be as great in the intralobular tissue as in the pleura, septa and other structures. Electron microscopic examination of the cells shows that they resemble true mast cells; some contain two types of granule, the appearance of which suggests that part of the amine has been discharged. Although in many places the cells are closely associated with adrenergic nerve fibres no evidence was found to suggest that they had any functional relation with one another.

Keywords

Dopamine Mast Cell Fluorescence Microscopy Nerve Fibre Microscopic Examination 

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References

  1. Coupland, R. E. (1963).Ann. N.Y. Acad. Sci. 103, 139–50.Google Scholar
  2. Coupland, R. E. &Heath, I. D. (1961). The chromaffin cells of the liver capsule and gut of ungulates.J. Endocr. 22, 71–6.Google Scholar
  3. Bertler, A., Falck, B., Hillarp, N. A., Rosengren, E. &Torp, A. (1959). Dopamine and chromaffin cells.Acta physiol. scand. 47, 251–8.Google Scholar
  4. Bloom, G. D. &Haegermark, Ö. (1965). A study of the morphological changes and histamine release induced by compound 40/80 in rat peritoneal mast cells.Expl. Cell Res. 40, 637–54.Google Scholar
  5. Falck, B., Hillarp, N. A. &Torp, A. (1959a). A new type of chromaffin cell probably storing dopamine.Nature, Lond. 183, 267–8.Google Scholar
  6. Falck, B., Hillarp, N. A. &Torp, A. (1959b). Some observations on the histology and histochemistry of the chromaffin cells probably storing dopamine.J. Histochem. Cytochem. 7, 323–8.Google Scholar
  7. Falck, B., Nystedt, T., Rosengren, E. &Stenflo, T. (1964). Dopamine and mast cells of the ruminants.Acta pharmac. tox. 21, 51–8.Google Scholar
  8. Hebb, C. &Mann, S. P. (1968).J. Anat. (In press.)Google Scholar
  9. Hebb, C., Mann, S. P. &Perkins, H. D. (1966). Histochemical demonstration of catecholamines and cholinesterases on adjacent sections of tissue.J. Physiol., Lond. 184, 12–13.Google Scholar
  10. Lewis, P. (1961). The effect of varying conditions in the Koelle technique.Biblthca anat. 2, 11–20.Google Scholar
  11. Millonig, G. (1962). Further observations on a phosphate buffer for osmium solutions in fixation. Fifth International Congress for Electron Microscopy, Vol. 2, p. 8 (ed. Sydney S. Breese). New York: Academic Press.Google Scholar
  12. Reynolds, E. S. (1963). The use of lead citrate of high pH as an electron-opaque stain in electron microscopy.J. Cell Biol. 17, 208–12.Google Scholar
  13. West, G. B. (1956). Histamine and mast cells. In:Histamine (Ciba Fdn., eds. G. E. W. Wolstenholme, & Cecilia M. O'Connor) London: Churchill.Google Scholar

Copyright information

© Chapman and Hall 1968

Authors and Affiliations

  • Catherine Hebb
    • 1
  • P. Kasa
    • 1
  • S. Mann
    • 1
  1. 1.A.R.C.Institute of Animal PhysiologyCambridge

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