Skip to main content
Log in

Mg2+-ATPase activity in the rat testis and its correlation with the stages of spermatogenesis: a histochemical study

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The activity of Mg2+-activated ATPase was studied in the rat testis employing histochemical and microphotometrical methods. The enzymatic activity could be correlated with the stages of spermatogenesis. From stage XII onwards, the elongated spermatids showed a weak to moderate activity, which increased from the acrosomal phase to the, maturation phase. The reaction product was located in the mid-piece and tail piece of elongated spermatids with a maximum intensity at stage VIII. Morphometric analysis revealed six distinct patterns, which could be correlated with the stages of spermatogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baust, P., Goslar, H. G. &Tonutti, E. (1967) Das Verhalten einiger Phosphatasen im Hoden von Ratte, Maus und Meerschweinchen nach Oestrogenbehandlung.Acta histochem. 26, 343–50.

    Google Scholar 

  • Bloom, W. &Fawcett, D. W. (1975)A Textbook of Histology Philadelphia, W. B. Saunders.

    Google Scholar 

  • Breitenecker, G., Böck, P. &Lunglmayr, G. (1974) Histochemical localization of phosphohydrolases in testes of man and rat.Z. Anat. Entwickl. Gesch. 143, 301–13.

    Google Scholar 

  • Ciplea, A. G., Metze, K., Grundmann, E., Roessner, A. &Hetiwer, H. (1985) Microphotometric quantitation of enzyme activities in giant cell tumor of bone.Path. Res. Pract. 179, 412–8.

    Google Scholar 

  • Dierichs, R. &Wrobel, K.-H. (1973) Licht-und elektronenmikroskopische Untersuchungen an den peritubulären Zellen des Schweinehodens während der postnatalen Entwicklung.Z. Anat. Entwickl. Gesch. 143, 49–64.

    Google Scholar 

  • Dierkes-Tizek, U., Glaser, U., Oldiges, H. &Hettwer, H. (1984) Wirkung von Organophosphaten auf Herz-ATPasen von Ratten.Arzneim-Forsch./Drug Res 34, 671–8.

    Google Scholar 

  • Haider, S. G., Passia, D., Stuhl, O. &Birkofer, L. (1984) Organosilicone induced malformation of rat sperms a histochemical study.Acta histochem 75, 149–51.

    Google Scholar 

  • Hettwer, H. (1984) The Ca2+-activated ATP-A in the tubuli seminiferi of rats.Cell. mol. Biol. 30, 391–9.

    Google Scholar 

  • Hilscher, B., Passia, D. &Hilscher, W. (1979) Kinetics of the enzymatic pattern in the testis. I. Stage dependence of enzymatic activity and its relation to cellular interactions in the testis of the Wistar rat.Andrologia 11, 169–81.

    Google Scholar 

  • Hofmann, N. &Haider, S. G. (1985) Neue Ergebnisse morphologischer Diagnostik der Spermatogene-sestörungen.Gynäkologe 18, 70–80.

    Google Scholar 

  • Jülich, G., Haider, S. G., Passia, D. &Goslar, H. G. (1982) Jahreszeitliche Veränderungen der Adenosintriphosphatase-Aktivität in den Germinalcysten des Testis vom GrasfroschRana temporaria Eine enzymhistochemische Studie,Acta histochem. 71, 191–200.

    Google Scholar 

  • Kalla, N. R. &Vasudev, M. (1981) Studies on the male antifertility agent gossypol acetic acid. II. Effect of gossypol acetic acid on the motility and ATPase activity of human spermatozoa.Andrologia,13, 95–98.

    Google Scholar 

  • Kalla, N. R., Vasudev, M. &Arora, G (1981) Studies on the male antifertility agent-gossypol acetic acid. III. Effect of gossypol acetic acid on rat testis.Andrologia,13, 242–9.

    Google Scholar 

  • Keyhani, E. &Storey, B. T. (1973) Energy conservation capacity in morphological integrity of mitochondria in hypotonically treated rabbit epididymal spermatozoa.Biochim. biophys. Acta 305, 557–69.

    Google Scholar 

  • Krause, W. &Peters, S. (1980) Zur ATP-Bestimmung im Sperma mit dem BiolumineszenzverfahrenZ. Zuchthygiene 2, 71.

    Google Scholar 

  • Lazarus, S. S. &Barden, H. (1962) Histochemistry and electron microscopy of mitochondrial adenosinetriphosphatase.J. Histochem. Cytochem. 10, 285–93.

    Google Scholar 

  • Lazarus, S. S. &Vethamany, V. G (1966) Fine structural localization of ATPase in mitochondria.J. Histochem. Cytochem. 14, 810.

    Google Scholar 

  • Leblond, C. P. &Clermont, Y. (1952) Definition of the stages of the cycle of seminiferous epithelium in the rat.Ann. N. Y. Acad. Sci,55, 548–73.

    Google Scholar 

  • Lojda, Z., Gossrau, R. &Schiebler, T. H. (1979)Enzyme Histochemistry. A Laboratory. Manual, pp. 93–100. Berlin: Springer-Verlag.

    Google Scholar 

  • Meijer, A. E. F. H. &Vloedman, A. H. T. (1980) The histochemical characterization of the coupling state of skeletal muscle mitochondria.Histochemistry,69, 217–32.

    Google Scholar 

  • Padykula, H. A. &Herman, E. (1955) The specificity of the histochemical method for adenosine triphosphatase.J. Histochem. Cytochem. 3, 170–95.

    Google Scholar 

  • Passia, D. (1985) Enzymhistochemische Untersuchungen zur Orthologie und Pathologie des menschlichen TestisgewebesEurop. Hochschulschr., Vol. 27, III. Frankfurt, Bern, New York: Lang-Verlag.

    Google Scholar 

  • Passia, D., Hilscher, B., Hoffmann, K., Haider, S. G. &Hilscher, W. (1982) Histologische und enzymhistochemische Untersuchungen am Testis des Djungarischen Hamsters, (Phodopus sungorus) unter Langtag-und Kurztag-Bedingungen.Verh. Anat. Ges. 76, 349–50.

    Google Scholar 

  • Wachstein M. &Meisel, E. (1957) Histochemistry of hepatic phosphatases at a physiologic pH with special reference to the demonstration of bile canaliculi.Am. J. clin. Path. 27, 13–23.

    Google Scholar 

  • Weinbauer, G. F., Rovan, E. &Frick, J. (1983) Die antifertilitäre Wirkung der Gossypol-Essigsäure in männlichen Ratten.Beitr., Urol. 3, 239–42.

    Google Scholar 

  • Wrobel, K.-H. &Kühnel, W. (1968) Enzymhistochemie am Hoden der Haussäugetiere. II. Hydrolasen im Hoden von Ziege und Schwein.Berl. Mnch. Tierärztl. Wschrift. 15, 297–301.

    Google Scholar 

  • Wrobel, K.-H. &Eletreby, M. F. (1971) Enzymhistotopochemie an der männlichen Keimdrüse des Rindes während ihrer fetalen und postnatalen Entwicklung.Histochemie 26, 160–79.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hettwer, H., Haider, S.G. & Passia, D. Mg2+-ATPase activity in the rat testis and its correlation with the stages of spermatogenesis: a histochemical study. Histochem J 17, 1299–1308 (1985). https://doi.org/10.1007/BF01002527

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002527

Keywords

Navigation