The Histochemical Journal

, Volume 15, Issue 10, pp 999–1009 | Cite as

Cytochemical glucose-6-phosphatase activity in the cells of mouse pancreas and submandibular gland

  • Jun Watanabe
  • Shinsuke Kanamura
  • Kazuo Kanai
  • Motoko Yoshikawa
  • Mari Asada-Kubota


Ultrastructural localization of glucose-6-phosphatase activity was studied in the cells of the pancreas and submandibular gland of the mouse using an incubation medium modified from that of Wachstein & Meisel (1956). In pancreatic acinar cells, the reaction product for the enzyme activity was not found even after 90 min of incubation with three changes of the medium. However, the reaction product was localized in the endoplasmic reticulum and nuclear envelope of all other cell types composing the pancreas and submandibular gland. The reaction product appeared in moderate to abundant amounts in acinar cells and striated duct cells of the submandibular gland, and in the B cells, A cells and D cells of the pancreatic islet, but it was scarce in other cell types.


Enzyme Activity Endoplasmic Reticulum Pancreatic Islet Acinar Cell Incubation Medium 
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Copyright information

© Chapman and Hall Ltd 1983

Authors and Affiliations

  • Jun Watanabe
    • 1
  • Shinsuke Kanamura
    • 1
  • Kazuo Kanai
    • 1
  • Motoko Yoshikawa
    • 1
  • Mari Asada-Kubota
    • 1
  1. 1.Department of AnatomyKansai Medical UniversityMoriguchi, OsakaJapan

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