The Histochemical Journal

, Volume 21, Issue 11, pp 638–644 | Cite as

Production of monoclonal antibodies to lactate dehydrogenase (LDH) isoenzymes for immunohistochemical study on fixed tissue section

  • Langxing Pan
  • Peter C. L. Beverley
  • Lynda G. Bobrow
  • Dallas M. Swallow
  • Peter G. Isaacson
Papers

Summary

Purified lactate dehydrogenase (LDH) isoenzyme 1 (H or B subunits) and isoenzyme 5 (M or A subunits) were used to prepare monoclonal antibodies (MAb) suitable for immunohistochemical detection on formalin fixed paraffin-embedded tissue sections. In the initial fusions, screening of the antibodies was based on enzyme linked immunosorbent assay (ELISA) against the immunogens. None of the antibodies obtained was satisfactory. There were various problems related to specificity, crossreactivity, affinity and also the properties of the monoclonal antibody itself. Using a combined system involving more than one method for screening, two suitable monoclonal antibodies, MAb65 (to H-type LDH) and MAb25 (to M-type LDH) were selected. Both antibodies reacted specifically with corresponding LDH isoenzymes as shown in a series of tests. Their reactivity in sections of formalin fixed paraffin-embedded tissue indicated that both antibodies are suitable reagents for immunohistochemical studies.

Keywords

Lactate Monoclonal Antibody Formalin Tissue Section Immunosorbent Assay 

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Copyright information

© Chapman and Hall Ltd. 1989

Authors and Affiliations

  • Langxing Pan
    • 1
  • Peter C. L. Beverley
    • 2
  • Lynda G. Bobrow
    • 1
    • 2
  • Dallas M. Swallow
    • 3
  • Peter G. Isaacson
    • 1
  1. 1.Department of HistopathologyUniversity College and Middlesex Medical SchoolLondon
  2. 2.I. C. R. F. Human Tumour Immunology GroupThe Courtauld Institute of BiochemistryLondon
  3. 3.MRC Human Biochemical Genetics Unit, The Galton LaboratoryUniversity College LondonLondon

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