Skip to main content
Log in

Simultaneous localization of acetylcholinesterase activity and met-enkephalin, vasoactive intestinal peptide and substance P immunoreactivity in the rat myenteric plexu

  • Short Communication
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Costa, M., Cuello, A. C., Furness, J. B. &Franco, R. (1980a) Distribution of neurons showing immunoreactivity for substance P in the guinea-pig ileum.Neuroscience 5, 323–31.

    Google Scholar 

  • Costa, M., Furness, J. B., Buffa, R. &Said, S. I. (1980b) Distribution of enteric nerve cell bodies and axons showing immunoreactivity for vasoactive intestinal polypeptide in the guinea-pig intestine.Neuroscience 5, 787–96.

    Google Scholar 

  • Furness, J. B., Costa, M. &Miller, R. J. (1983a) Distribution and projections of nerves with enkephalin-like immunoreactivity in the guinea-pig small intestine.Neuroscience 8, 653–64.

    Google Scholar 

  • Furness, J. B., Costa, M. &Eckenstein, F. (1983b) Neurones localized with antibodies against choline acetyltransferase in the enteric nervous system.Neurosci. Lett. 40, 105–9.

    Google Scholar 

  • Graham, R. C. &Karnovsky, M. J. (1966) The early stages of absorption of injected horseradish peroxydase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291–302.

    Google Scholar 

  • Ito, Y., Sohma, S. &Hirano, H. (1984) Light and electron microscopic studies on acetylcholinesterase activity in Auerbach's plexus of the developing rat colon.Histochemistry 81, 209–12.

    Google Scholar 

  • Karnovsky, M. J. &Roots, L. (1964) A “direct-coloring” thiocholine method for cholinesterases.J. Histochem. Cytochem. 12, 219–21.

    Google Scholar 

  • Kobayashi, S., Suzuki, M., Uchida, T. &Yanaihara, N. (1984) Enkephalin neurons in the guinea-pig duodenum: a light and electron microscopic immunocytochemical study using an antiserum to methionine-enkephalin-Arg6-Gly7-Leu8.Biomed. Res. 5, 489–506.

    Google Scholar 

  • Koelie, G. B. (1955) The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons.J. Pharmacol. Exptl. Therap. 114, 167–84.

    Google Scholar 

  • Koelle, G. B. &Friedenwald, J. S. (1949) A histochemical method for localizing cholinesterase activity.Proc. Soc. exp. Biol. (N.Y.) 70, 617–22.

    Google Scholar 

  • Larsson, L.-I. (1977) Ultrastructural localization of a new neuronal peptide (VIP).Histochemistry,54, 173–6.

    Google Scholar 

  • Lehrer, G. M. &Ornstein, L. (1959) A diazo coupling method for the electron microscopic localization of cholinesterase.J. Bioph. Biochem. Cytol. 6, 399–406.

    Google Scholar 

  • Levey, A. I., Wainer, B. H., Mufson, E. J. &Mesulam, M. M. (1983) Co-localization of acetylcholinesterase and choline acetyltransferase in the rat cerebrum.Neuroscience 9, 9–22.

    Google Scholar 

  • Nilsson, G., Larsson, L.-I., Hakanson, R., Brodin, E., Fernow, B. &Sundler, F. (1975) Localization of substance P-like immunoreactivity in the mouse gut.Histochemistry 43, 97–9.

    Google Scholar 

  • Pearse, A. G. E. &Polak, J. M. (1975) Immunocytochemical localization of substance P in mammalian intestine.Histochemistry 41, 373–5.

    Google Scholar 

  • Polak, J. M., Sullivan, S. N., Bloom, S. R., Facer, P. &Pearse, A. G. E. (1977) Enkephalin-like immunoreactivity in the human gastrointestinal tract.Lancet 1, 972–4.

    Google Scholar 

  • Sternberger, L. A., Hardy, P. H., Cuculis, J. J. &Meyer, M. G. (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem. 18, 315–33.

    Google Scholar 

  • Taxi, J. (1965) Contribution à l'étude des connexions des neurones moteurs du système nerveux autonome.Ann. Sci. Nat. Zool. 7, 413–674.

    Google Scholar 

  • Tsuji, S. (1974) On the chemical basis of thiocholine methods for demonstration of acetylcholinesterase activity.Histochemistry 42, 99–110.

    Google Scholar 

  • Tsuji, S. (1986) Histochemical demonstration of membrane-bound acetylcholinesterase: A methodological consideration on Koelle's copper thiocholine reaction and on Karnovsky's copper ferrocyanide reaction.Acta histochem., Suppl.-Band XXXIII, 147–56.

    Google Scholar 

  • Tsuji, S. &Larabi, Y. (1983) A modification of thiocholine-ferrocyanide method of Karnovsky and Roots for localization of acetylcholinesterase activity without interference by Koelle's copper thiocholine iodide precipitate.Histochemistry 78, 317–23.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Anglade, P., Michel, C., Ozaki, T. et al. Simultaneous localization of acetylcholinesterase activity and met-enkephalin, vasoactive intestinal peptide and substance P immunoreactivity in the rat myenteric plexu. Histochem J 20, 464–467 (1988). https://doi.org/10.1007/BF01002433

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002433

Keywords

Navigation