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The Histochemical Journal

, Volume 20, Issue 8, pp 455–463 | Cite as

Quantitative enzyme histochemistry of rat foetal brain and trigeminal ganglion

  • P. Chieco
  • P. Hrelia
  • G. Lisignoli
  • G. Cantelli-forti
Papers

Summary

The increasing concern and the efforts in determining neurological effects in offsprings resulting from maternal exposure to xenobiotics are faced with several difficulties in monitoring damage to the central nervous system. In this paper, the efficiency of several enzyme histochemical reactions for analysing the forebrain and the trigeminal ganglia of rat foetuses are reported. Brains of 20-day-old Sprague-Dawley rat foetuses were frozen and analysed for 18 enzymes that had previously been used to monitor initial injury caused by toxic compounds in liver and other organs. Eight enzymes appeared suitable as histochemical markers for the functional integrity of different areas in brain and ganglia of rats exposed to xenobiotics. They were lactate, malate, glycerophosphate (NAD-linked), succinate, aldehyde and glucose 6-phosphate dehydrogenases, α-glycerophosphate-menadione oxidoreductase and cytochromec oxidase. The activities of the enzymes were determined by microphotometry and the arrangement of absorbances of the enzyme final reaction products into appropriate analytical tables is proposed as an efficient procedure for data analysis.

Keywords

Succinate Cytochromec Oxidase Trigeminal Ganglion Foetal Brain Neurological Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AcChE

acetylcholinesterase

AldDH

aldehyde dehydrogenase

ALKPase

alkaline phosphatase

5′AMPase

adenosine monophosphatase

ATPase

Mg2+ dependent adenosine triphosphatase

CytOx

cytochromec oxidase

GAPDH

glyceraldehyde phosphate dehydrogenase

GIDH

glutamate dehydrogenase

GLPDH

glycerophosphate: NAD oxidoreductase

CPODH

glycerophosphate:menadione oxidoreductase

G6Pase

glucose-6-phosphatase

G6PDH

glucose-6-phosphate dehydrogenase

IDH

lactate dehydrogenase

MaDH

malate dehydrogenase

MAO

monoamine oxidase

NADPH, DH, NADPH

tetrazolium oxidoreductase

SuDH

succinate dehydrogenase

6PGDH

6-phosphogluconate dehydrogenase

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Copyright information

© Chapman and Hall Ltd. 1988

Authors and Affiliations

  • P. Chieco
    • 1
  • P. Hrelia
    • 2
  • G. Lisignoli
    • 1
  • G. Cantelli-forti
    • 2
  1. 1.Institute of Oncology'F. Addarii'BologneItaly
  2. 2.Institute of PharmacologyUniversity of BolognaItaly

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