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The Histochemical Journal

, Volume 20, Issue 8, pp 427–432 | Cite as

LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney

  • R. Herken
  • M. Fussek
  • S. Barth
  • W. Götz
Papers

Summary

This work describes a method for the immunolocalization of laminin on 1μm-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50°C whereas LR-Gold was polymerized at −25°C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.

Keywords

Tissue Section Acrylic Resin Laminin Ethanol Concentration Immunofluorescence Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1988

Authors and Affiliations

  • R. Herken
    • 1
  • M. Fussek
    • 1
  • S. Barth
    • 1
  • W. Götz
    • 1
  1. 1.Zentrum Anatomie, Abteilung HistologieGeorg-August Universität GöttingenGöttingenGermany

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