Abstract
Freeze-dried sections (14 μm thick) were prepared from mice with normal (C57BL strain) and degenerated (C3H strain) retinas. GABA concentration and GAD activity were determined in the microsamples (1.8–20 ng dry weight) of retinal layers and sublayers, using an enzymatic amplication reaction, NADP cycling. 1) GABA was distributed over all layers of normal retina with a broad concentration peak covering both inner nuclear and plexiform layers. In contrast, GAD activity was mostly localized in the inner plexiform layer. 2) GABA concentration was similar in one-fourth of the sublayers of each inner nuclear or plexiform layer. GAD activity was highest in the innermost sublayer of the inner nuclear layer. An increasing gradient of GAD activity was present in the inward direction in the inner plexiform layer. 3) In the degenerated retina, lacking in photoreceptors, the inner nuclear and plexiform layers remained, and GABA and GAD levels in these layers were similar to those in normal retina.
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Agardh, E., Bruun, A., Ehinger, B., and Strom-Mathisen, J. 1986 Gaba immunoreactivity in the retina. Invest. Ohpthalmol. Vis. Sci. 27:674–678.
Berger, S. J., McDaniel, M. L., Carter, J. G., and Lowry, O. H. 1977 Distribution of four potential transmitter amino acids in monkey retina. J. Neurochem. 28:159–163.
Brandon, C. 1985 Retinal GABA neurons: localization in vertebrate species using an antiserum to rabbit brain glutamate decarboxylase. Brain Res. 344:286–295.
Brandon, C., Lam, D. M. K., and Wu, J.-Y. 1979 The γ-aminobutyric acid system in rabbit retina: localization by immunocytochemistry and autoradiography. Proc. Natl. Acad. Sci. U.S.A. 76:3557–3561.
Chi, M. M.-Y., Lowry, C. V., and Lowry, O. H. 1978 An improved enzymatic cycle for nicotinamide-adenine dinucleotide phosphate. Anal. Biochem. 89:119–129.
Cohen, A. I., McDaniel, M., and Orr, H. 1973 Absolute levels of some free amino acid in normal and biologically fractionated retinas. Invest. Ophthalmol. 12:686–693.
Dick, E., and Lowry, O. H. 1984 Distribution of glycine, γ-aminobutyric acid, glutamate decarboxylase, and γ-aminobutyric acid transaminase in rabbit and mudpuppy retinas. J. Neurochem. 42:1274–1280.
Dubin, M. W. M. 1974 Anatomy of the vertebrate retina. Pages 227–256, in Davison, H. and Graham, L. T. Jr. (eds.), The Eye, vol 6, Academic Press, New York.
Graham, L. T. Jr. 1972 Intraretinal distribution of GABA content and GAD activity. Brain Res. 36:476–479.
Ehinger, B. 1983 Connexions between retinal neurons with identified neurotransmitters. Vision Res. 23:1281–1291.
Hirsch, H. E., and Robins, E. 1962 Distribution of γ-aminobutyric acid in the layers of the cerebral and cerebellar cortex. Implications for its physiological role. J. Neurochem. 9:63–70.
Kuriyama, K., Sisken, B., Haber, B., and Roberts, E. 1968 The γ-aminobutyric acid system in rabbit retina. Brain Res. 9:165–168.
Lam, D. M. K., Su, Y. Y. T., Swain, L., Marc, R. E., Brandon, C. and Wu, J.-Y. 1979 Immunocytochemical localisation ofl-glutamic acid decarboxylase in the goldfish retina. Nature, 278:565–567.
Lowry, O. H., Roberts, N. R., and Lewis, C. 1956 The quantitative histochemistry of the retina. J. Biol. Chem. 220:879–892.
Lowry, O. H. and Passonneau, J. V. 1972 A flexible system of enzymatic analysis. Academic Press, New York and London.
Macaione, S. 1972 Localization of GABA system in rat retina. J. Neurochem. 19:1397–1400.
Marc, R. E. 1986 Neurochemical stratification in the inner plexiform layer of the vertebrate retina. Vision Res. 26:223–238.
Matuk, Y. 1984 Retinitis pigmentosa and retinal degeneration in animals: a review. Can. J. Biochem. Cell Biol. 62:535–546.
Mosinger, J. L., and Yazulla, S. 1985 Colocalization of GAD-like immunoreactivity and3H-GABA uptake in amacrine cells of rabbit retina. J. Comp. Neurol. 240:396–406.
Murashima, Y. L., and Kato, T. 1984 An improved enzymatic microassay method for glutamate decarboxylase. Biomed. Res. 5:527–532.
Murashima, Y. L., and Kato, T. 1985 Glutamate decarboxylase activities in single vertebrate neurons. J. Neurochem. 45:732–738.
Murashima, Y. L., and Kato, T. 1986 Distribution of γ-aminobutyric acid and glutamate decarboxylase in the layers of rat oviduct. J. Neurochem. 46:166–172.
Nakamura, Y., McGuire, B. A., and Sterling, P. 1980 Interplexiform cell in cat retina: identification by uptake of γ-[3H]aminobutyric acid and serial reconstruction. Proc. Natl. Acad. Sci. USA, 77:658–661.
Noell, W. K. 1958 Differentiation, metabolic organization, and viability of the visual cell. A. M. A. Archiv. Ophthal. 60:702–733.
Orr, H. T., Cohen, A. I., and Carter, J. A. 1976 The levels of free taurine, glycine and γ-amino butyric acid during the postnatal developing of the normal and dystrophic retina of the mouse. Exp. Eye Res. 23:377–384.
Pusateri, M. E., Carter, J. G., Berger, S. J., and Lowry, O. H. 1984 Distribution of three enzymes of γ-aminobutyric acid metabolism in monkey retina. J. Neurochem. 42:1269–1273.
Sarthy, P. V. 1982 The uptake of [3H] γ-aminobutyric acid by isolated glial (Muller) cells from the mouse retina. J. Neurosci. Methods 5:77–82.
Schnitzer, J. and Rusoff, A. C. 1984 Horizontal cells of the mouse retina contain glutamic acid decarboxylase-like immunoreactivity during early developmental stages. J. Neurosci. 4:2948–2955.
Vaughn, J. E., Famiglietti, E. V. Jr., Barber, R. P., Saito, K., Roberts, E., and Ribak, C. E. 1981 GABAergic amacrine cells in rat retina: immunocytochemical identification and synaptic connectivity. J. Comp. Neurol. 197:113–127.
Zucker, C., Yazulla, S., and Wu, J.-Y. 1984 Non-correspondence of [3H]GABA uptake and GAD localization in goldfish amacrine cells. Brain Res. 298:154–158.
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Special Issue dedicated to Dr. O. H. Lowry.
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Kato, T., Murashima, Y.L. GABA concentration and GAD activity levels in normal and degenerated retinas from mice. Neurochem Res 12, 529–535 (1987). https://doi.org/10.1007/BF01000237
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DOI: https://doi.org/10.1007/BF01000237