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Glucose metabolism and acidosis in the metabolic penumbra of rat brain

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Abstract

The characterization of tissue acid-base status related to the penumbral zone of increased glucose consumption surrounding a focal cerebral ischemic lesion may suggest therapeutic techniques to maximize tissue survivability from stoke. We measured local cerebral metabolic rate for glucose (l CMRglc) and an index of brain tissue pH (pHt) concurrently and characterized their interaction in a model of focal cerebral ischemia in rats in a double-label autoradiographic study, using [14C]2-deoxyglucose and [14C]dimethyloxazolidinedione. Computer-assisted digitization and analysis permitted the simultaneous quantification of the two variables on a pixel-by-pixel basis in the same brain slices. Hemispheres ipsilateral to intravascular tamponade-induced middle cerebral artery occlusion showed areas of normal, depressed, and elevated glucose metabolic rate (as defined by an interhemispheric asymmetry index) after 2 hr of ischemia. Regions of increased l CMRglc showed moderate acidosis (6.87±0.05), while regions of normal glucose metabolic rate showed normal pHt (pH±SD=6.98 ± 0.05) and regions of decreased l CMRglc showed severe acidosis (6.69±0.11). A repeated-measures analysis of variance found these values to differ from each other at theP < 0.0005 significance level. The finding of moderate acidosis coupled with increased l CRMglc in the metabolic penumbra suggests that the excess protons may result from the anaerobic dissociation of ATP synthesis and hydrolysis.

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Authors and Affiliations

  1. Department of Neurology and Positron Diagnostic and Research Center, University of Texas Health Science Center at Houston, 77030, Houston, Texas

    Kathryn E. Peek, Alan H. Lockwood, Masahiro Izumiyama, Eddy W. H. Yap & Jocelyn Labove

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  1. Kathryn E. Peek
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  2. Alan H. Lockwood
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  3. Masahiro Izumiyama
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  4. Eddy W. H. Yap
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  5. Jocelyn Labove
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Peek, K.E., Lockwood, A.H., Izumiyama, M. et al. Glucose metabolism and acidosis in the metabolic penumbra of rat brain. Metab Brain Dis 4, 261–272 (1989). https://doi.org/10.1007/BF00999772

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