Metabolic Brain Disease

, Volume 2, Issue 3, pp 207–212 | Cite as

Disposition of homovanillic acid in the primate

  • Alexander L. Miller
  • Roy W. Keenan
  • James W. Maas
  • Ricardo H. Asch
Original Contributions

Abstract

Prior studies have shown that homovanillic acid is the principal metabolite of dopamine in the primate central nervous system (CNS). In studies of primates given deuterated homovanillic acid systemically, however, only 50% of the administered amounts have been recovered in the urine over the next 4–48 hr. These findings have left it unclear whether there is a slowly turning-over compartment of homovanillic acid, conversion of homovanillic acid to another compound, or excretion of homovanillic acid from the body by a nonrenal route. We synthesized [3H]homovanillic acid and administered it intravenously to four rhesus monkeys. Over the subsequent 4 hr, 94.9 ± 8.9% (SD) of the administered radioactivity was recovered in the urine, almost entirely as homovanillic acid. These results are consistent with the interpretation that, in primates, there is not a major body pool of homovanillic acid with slow turnover, nor is metabolism to other compounds significant, nor is there evidence for nonrenal excretion.

Key words

homovanillic acid dopamine primate metabolism urine 

Abbreviations used

DA

dopamine

DOPAC

dihydroxyphenylacetic acid

HVA

homovanillic acid

CNS

central nervous system

HPLC

high-performance liquid chromatography

NMR

nuclear magnetic resonance

UV

ultraviolet

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anggard, E., Lewander, T., and Sjoquist, B. (1974). Determination of homovanillic acid turnover in man.Life Sci. 15: 111–172.Google Scholar
  2. Bacopoulos, N. G., Maas, J. W., Hattox, S. E., and Roth, R. H. (1978). Regional distribution of dopamine metabolites in human and primate brain.Commun. Psychopharmacol. 2: 281–287.Google Scholar
  3. Elchisak, M. A., Polinsky, R. J., Ebert, M. H., Powers, K. J., and Kopin, L. J. (1979). Contribution of plasma homovanillic acid (HVA) to urine and cerebrospinal fluid HVA in the monkey and its pharmacokinetic disposition.Life Sci. 23: 2339–2348.Google Scholar
  4. Elchisak, M. A., Polinsky, R. J., Ebert, M. H., and Kopin, I. J. (1982). Kinetics of homovanillic acid and determination of its production rate in humans.J. Neurochem. 38: 380–385.Google Scholar
  5. Keenan, R. W. (1987). A simple procedure for the preparation of tritiated homovanillic acid.J. Labelled Compounds Radiopharm. (in press).Google Scholar
  6. Lindstrom, B., Sjoquist, B., and Anggard, E. (1974). Preparation of deuterium labelled catecholamines, catecholamine precursors and metabolites for use as internal standards in mass fragmentographic determinations and for turnover studies.J. Labelled Compounds 10: 187–194.Google Scholar
  7. Maas, J. W., Hattox, S. E., Martin, D. M., and Landis, D. H. (1979). A direct method for determining dopamine synthesis and output of dopamine metabolites from brain in awake animals.J. Neurochem. 32: 839–843.Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Alexander L. Miller
    • 1
  • Roy W. Keenan
    • 2
  • James W. Maas
    • 1
  • Ricardo H. Asch
    • 3
  1. 1.Department of PsychiatryThe University of Texas Health Science CenterSan Antonio
  2. 2.Department of BiochemistryThe University of Texas Health Science CenterSan Antonio
  3. 3.Department of Obstetrics and GynecologyThe University of Texas Health Science CenterSan Antonio

Personalised recommendations