Planta

, Volume 159, Issue 1, pp 66–76 | Cite as

Photosynthetic characteristics of chloroplasts isolated fromMesembryanthemum crystallinum L., a halophilic plant capable of Crassulacean acid metabolism

  • Barbara Demmig
  • Klaus Winter
Article

Abstract

Photosynthetically highly active chloroplasts were routinely obtained by rupture of leaf protoplasts from the halophyteMesembryanthemum crystallinum which exhibited the photosynthetic characteristics of either a C3 plant when grown with 20 mmol l-1 NaCl in the rooting medium, or a Crassulacean-acid-metabolism (CAM) plant when grown with 400 mmol l-1 NaCl. Photosynthesis rates of C3 and CAM chloroplasts were 150–250 and 90–150 μmol mg-1 chlorophyll h-1, respectively. Because of osmotic adjustment, CAM chloroplasts required higher sorbitol concentrations (0.7–0.8 mol l-1) in the assay medium than C3 chloroplasts (0.3–0.4 mol l-1) for optimum activity. Substitution of sorbitol by NaCl as the osmoticum strongly reduced photosynthesis of CAM chloroplasts. Rates of electron transport (ferricyanide reduction, uncoupled) remained unaffected over a range of sorbitol concentrations (0 to 1 mol l-1). Sensitivity of electron transport to increasing levels of NaCl was less pronounced than the NaCl-sensitivity of CO2 fixation by intact chloroplasts. The CAM chloroplasts showed a broad pH optimum of photosynthesis between pH 7.0 and 8.2; photosynthesis of C3 chloroplasts dropped markedly below pH 7.6. The CAM chloroplasts maintained a higher transenvelope proton gradient than C3 chloroplasts both in the light and dark. External pyruvate (5 mmol l-1) inhibited photosynthesis of CAM chloroplasts, but not of C3 chloroplasts. Inhibition was reduced by increased external concentrations of orthophosphate.

Key words

Chloroplasts Crassulacean acid metabolism (inducible) Mesembryanthemum Photosynthesis (isolated chloroplasts) Salinity 

Abbreviations

CAM

Crassulacean acid metabolism

Chl

chlorophyll

EDTA

ethylenediaminetetraacetate

Hepes

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

PGA

3-phosphoglycerate

Tris

2-amino-2-(hydroxymethyl)-1,3-propanediol

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Barbara Demmig
    • 1
  • Klaus Winter
    • 1
  1. 1.Lehrstuhl für Botanik I and Lehrstuhl für Botanik II der UniversitätWürzburgFederal Republic of Germany

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