Planta

, Volume 159, Issue 1, pp 60–65 | Cite as

Synthesis of the major storage protein, hordein, in barley

Pulse-labeling study of grain filling in liquid-cultured detached spikes
  • Henriette Giese
  • Bente Andersen
  • Hans Doll
Article

Abstract

A liquid culture system for culturing detached spikes of barley (Hordeum vulgare L.) at different nutritional levels was established. The synthesis of hordein polypeptides was studied by pulse-labeling with [14C]sucrose at different stages of development and nitrogen (N) nutrition. All polypeptides were synthesised at 10 d after anthesis and hercafter an increase was observed for all polypeptides. A fivefold increase in total hordein was observed within the N range tested. Hordein-1 increased considerably more than hordein-2 with increased N nutrition, and hordein-1 synthesis exceeded that of hordein-2 at the highest N level 20 and 25 d after anthesis. Hordein-1 thus appears to act as the main N sink at high N levels. The synthesis of the major groups of hordein-2 polypeptides responded differently to increasing N in that the slower-migrating polypeptides increased more with increasing N than the faster-migrating polypeptides.

Key words

Grain filling Hordein Hordeum (storage protein) Nitrogen nutrition Seed protein synthesis 

Abbreviation

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Henriette Giese
    • 1
  • Bente Andersen
    • 1
  • Hans Doll
    • 1
  1. 1.Agricultural Research DepartmentRisø National LaboratoryRoskildeDenmark

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