Abstract
When care was taken to minimise the effects of phosphatase activity during extraction ofVinca rosea crown-gall tumour tissue, a large proportion of extractable cytolinin activity was present in the nucleotide fraction. Analysis using ion-exchange chromatography followed by enzymic or chemical degradation and subsequent identification of the biologically active material indicated that this activity was due to zeatin riboside 5′-monophosphate. This was also the major radiolabelled cytokinin formed when this tissue was supplied with [14C]adenine. The incorporation of radioactivity from [14C]adenosine into free cytokinins was also shown, but no incorporation of radioactivity was found when [3H]mevalonic acid lactone was supplied to this tissue under the same conditions. In parallel experiments using normal stem callus tissue ofV. rosea, no incorporation of [14C]adenine into free cytokinins was observed. The significance of these results is discussed in relation to a possible transfer-RNA-independent pathway of cytokinin biosynthesis, operating primarily at the mononucleotide level.
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Abbreviations
- DEAE:
-
diethylaminoethyl cellulose
- HPLC:
-
high-performance liquid chromatography
- TEAB:
-
triethylamonium bicarbonate
- TLC:
-
thin-layer chromatography
- ZR:
-
zeatin riboside
- ZMP:
-
zeatin riboside 5′-monophosphate
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Palni, L.M.S., Horgan, R., Darrall, N.M. et al. Cytokinin biosynthesis in crown-gall tissue ofVinca rosea . Planta 159, 50–59 (1983). https://doi.org/10.1007/BF00998814
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DOI: https://doi.org/10.1007/BF00998814