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The immunoassay of gibberellins

II. Quantitation of GA3, GA4 and GA7 by ultra-sensitive solid-phase enzyme immunoassays

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Abstract

The development of sensitive and specific solid-phase enzyme immunoassays for gibberellic acid and gibberellins A4 and A7 is reported. The use of antisera of high apparent affinity (Ka over 1010 l mol-1) in conjunction with alkaline phosphatase-labeled gibberellins allows, with minimum procedural effort, the quantitative determination of sub-picogram amounts of these gibberellins. The assays reported here are applicable to most gibberellins and can be set up with 1–1.5 mg of starting material. They represent the most sensitive methods for gibberellin determination known.

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Abbreviations

GA:

gibberellin

GA3 :

gibberellic acid

TLC:

thin-layer-chromatography

References

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Authors and Affiliations

  1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, Postfach 102148, D-4630, Bochum, Federal Republic of Germany

    Rainer Atzorn & Elmar W. Weiler

Authors
  1. Rainer Atzorn
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  2. Elmar W. Weiler
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Atzorn, R., Weiler, E.W. The immunoassay of gibberellins. Planta 159, 7–11 (1983). https://doi.org/10.1007/BF00998807

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  • DOI: https://doi.org/10.1007/BF00998807

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