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The immunoassay of gibberellins

I. radioimmunoassays for the gibberellins A1, A3, A4, A7, A9 and A20

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Abstract

Immunological assays for the detection of picogram amounts of gibberellins A1, A3, A4, A7, A9 and A20 are described. Antisera of high affinity (Ka over 1010 l mol-1) were raised in rabbits by immunization against carboxyl-coupled gibberellin-bovine-serum-albumin conjugates and used in combination with tritiated gibberellins or gibberellin derivatives of high specific radioactivity (over 1014 Bq mol-1). The assays allow gibberellin quantitation in as little as 1 mg of vegetative plant tissue.

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Abbreviations

GA:

gibberellin

GA3 :

gibberellic acid

TLC:

thin-layer chromatography

References

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Authors and Affiliations

  1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, Postfach 102148, D-4630, Bochum, Federal Republic of Germany

    Rainer Atzorn & Elmar W. Weiler

Authors
  1. Rainer Atzorn
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  2. Elmar W. Weiler
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Atzorn, R., Weiler, E.W. The immunoassay of gibberellins. Planta 159, 1–6 (1983). https://doi.org/10.1007/BF00998806

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  • DOI: https://doi.org/10.1007/BF00998806

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